Biogas Biocatalysis: Methanotrophic Bacterial Cultivation, Metabolite Profiling, and Bioconversion to Lactic Acid

Henard, Calvin A.; Franklin, Tyler G.; Youhenna, Batool; But, Sergey; Alexander, Danny; Kalyuzhnaya, Marina G.; Guarnieri, Michael T.

doi:10.3389/fmicb.2018.02610

Title: Biogas Biocatalysis: Methanotrophic Bacterial Cultivation, Metabolite Profiling, and Bioconversion to Lactic Acid

Journal Article · Wed Oct 31 00:00:00 EDT 2018 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2018.02610· OSTI ID:1480140

Henard, Calvin A.; Franklin, Tyler G.; Youhenna, Batool; But, Sergey; Alexander, Danny; Kalyuzhnaya, Marina G.; Guarnieri, Michael T.

Anaerobic digestion (AD) of waste substrates, and renewable biomass and crop residues offers a means to generate energy-rich biogas. However, at present, AD-derived biogas is primarily flared or used for combined heat and power (CHP), in part due to inefficient gas-to-liquid conversion technologies. Methanotrophic bacteria are capable of utilizing methane as a sole carbon and energy source, offering promising potential for biological gas-to-liquid conversion of AD-derived biogas. Here, we report cultivation of three phylogenetically diverse methanotrophic bacteria on biogas streams derived from AD of a series of energy crop residues. Strains maintained comparable central metabolic activity and displayed minimal growth inhibition when cultivated under batch configuration on AD biogas streams relative to pure methane, although metabolite analysis suggested biogas streams increase cellular oxidative stress. In contrast to batch cultivation, growth arrest was observed under continuous cultivation configuration, concurrent with increased biosynthesis and excretion of lactate. As a result, we examined the potential for enhanced lactate production via the employ of a pyruvate dehydrogenase mutant strain, ultimately achieving 0.027 g lactate/g DCW/h, the highest reported lactate specific productivity from biogas to date.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: FOA-0001085; AC36-08GO28308

OSTI ID:: 1480140

Alternate ID(s):: OSTI ID: 1484340

Report Number(s):: NREL/JA-5100-72861; 2610

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 9; ISSN 1664-302X

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

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Cited by: 32 works

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Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts Henard, Calvin A.; Akberdin, Ilya R.; Kalyuzhnaya, Marina G. Green Chemistry, Vol. 21, Issue 24 https://doi.org/10.1039/c9gc03722e	journal	January 2019
Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution Lee, Jong Kwan; Kim, Sujin; Kim, Wonsik Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1574-9	journal	September 2019

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Title: Biogas Biocatalysis: Methanotrophic Bacterial Cultivation, Metabolite Profiling, and Bioconversion to Lactic Acid

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