Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

Hovde, Blake T.; Hanschen, Erik R.; Steadman Tyler, Christina R.; Lo, Chien-Chi; Kunde, Yuliya; Davenport, Karen; Daligault, Hajnalka; Msanne, Joseph; Canny, Stephanie; Eyun, Seong-il; Riethoven, Jean-Jack M.; Polle, Juergen; Starkenburg, Shawn R.

doi:10.1016/j.algal.2018.09.012

Title: Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

Journal Article · Mon Oct 01 00:00:00 EDT 2018 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2018.09.012· OSTI ID:1479995

Hovde, Blake T. ^[1]; Hanschen, Erik R. ^[1]; Steadman Tyler, Christina R. ^[1]; Lo, Chien-Chi ^[1]; Kunde, Yuliya ^[1]; Davenport, Karen ^[1]; Daligault, Hajnalka ^[1];

^[2]; Canny, Stephanie ^[3];

^[4]; Riethoven, Jean-Jack M. ^[5]; Polle, Juergen ^[6]; Starkenburg, Shawn R. ^[1]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Univ. of Nebraska, Lincoln, NE (United States). Center for Plant Science Innovation. School of Natural Resources
Univ. of Nebraska, Lincoln, NE (United States). Center for Biotechnology
Chung-Ang Univ., Seoul (Korea, Republic of). Dept. of Life Science
Univ. of Nebraska, Lincoln, NE (United States). Center for Biotechnology. School of Biological Sciences
CUNY Brooklyn College, New York, NY (United States)

Selection of highly productive algal strains is crucial for establishing economically viable biomass and bioproduct cultivation systems. Characterization of algal genomes, including understanding strain-specific differences in genome content and architecture is a critical step in this process. Using genomic analyses, we demonstrate significant differences between three strains of Chlorella sorokiniana (strain 1228, UTEX 1230, and DOE1412). We found that unique, strain-specific genes comprise a substantial proportion of each genome, and genomic regions with >80% local nucleotide identity constitute <15% of each genome among the strains, indicating substantial strain specific evolution. Furthermore, cataloging of meiosis and other sex-related genes in C. sorokiniana strains suggests strategic breeding could be utilized to improve biomass and bioproduct yields if a sexual cycle can be characterized. Finally, preliminary investigation of epigenetic machinery suggests the presence of potentially unique transcriptional regulation in each strain. Our data demonstrate that these three C. sorokiniana strains represent significantly different genomic content. Based on these findings, we propose individualized assessment of each strain for potential performance in cultivation systems.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1479995

Report Number(s):: LA-UR-18-24143

Journal Information:: Algal Research, Vol. 35; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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