Rapid Response of Eastern Mediterranean Deep Sea Microbial Communities to Oil

Liu, Jiang; Techtmann, Stephen M.; Woo, Hannah L.; Ning, Daliang; Fortney, Julian L.; Hazen, Terry C.

doi:10.1038/s41598-017-05958-x

Title: Rapid Response of Eastern Mediterranean Deep Sea Microbial Communities to Oil

Journal Article · Tue Jul 18 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-05958-x· OSTI ID:1394187

Liu, Jiang ^[1]; Techtmann, Stephen M. ^[2]; Woo, Hannah L. ^[1]; Ning, Daliang ^[3]; Fortney, Julian L. ^[1];

^[4]

Univ. of Tennessee, Knoxville, TN (United States)
Michigan Technological Univ., Houghton, MI (United States)
The Univ. of Oklahoma, Norman, OK (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Deep marine oil spills like the Deepwater Horizon (DWH) in the Gulf of Mexico have the potential to drastically impact marine systems. Crude oil contamination in marine systems remains a concern, especially for countries around the Mediterranean Sea with off shore oil production. The goal of this study was to investigate the response of indigenous microbial communities to crude oil in the deep Eastern Mediterranean Sea (E. Med.) water column and to minimize potential bias associated with storage and shifts in microbial community structure from sample storage. 16S rRNA amplicon sequencing was combined with GeoChip metagenomic analysis to monitor the microbial community changes to the crude oil and dispersant in on-ship microcosms set up immediately after water collection. After 3 days of incubation at 14 °C, the microbial communities from two different water depths: 824 m and 1210 m became dominated by well-known oil degrading bacteria. The archaeal population and the overall microbial community diversity drastically decreased. Similarly, GeoChip metagenomic analysis revealed a tremendous enrichment of genes related to oil biodegradation, which was consistent with the results from the DWH oil spill. These results highlight a rapid microbial adaption to oil contamination in the deep E. Med., and indicate strong oil biodegradation potential.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1394187

Alternate ID(s):: OSTI ID: 1479356

Journal Information:: Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 19 works

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Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities Miller, John I.; Techtmann, Stephen; Fortney, Julian Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00995	journal	May 2019
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