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Title: Both NaCl and H 2O 2 Long-Term Stresses Affect Basal Cytosolic Ca 2+ Levels but Only NaCl Alters Cytosolic Ca 2+ Signatures in Arabidopsis

Abstract

Salinity is one of the formidable environmental factors that affect plant growth and development and constrain agricultural productivity. Experimentally imposed short-term NaCl treatment triggers a transient increase in cytosolic free Ca 2+ concentration ([Ca 2+] i) via Ca 2+ influx across the plasma membrane. Salinity stress, as well as other stresses, induces the production of reactive oxygen species (ROS), such as H 2O 2. It is well established that short-term H 2O 2 treatment also triggers a transient increase in [Ca 2+] i. However, whether and how long-term NaCl and H 2O 2 treatments affect the basal levels of [Ca 2+] i as well as plant responses to additional NaCl and H 2O 2 stresses remain poorly understood. Using an aequorin-based Ca 2+ imaging assay, we found that the long-term treatment of Arabidopsis seedlings with both moderate NaCl and H 2O 2 in the growth media reduced the basal [Ca 2+] i levels. Interestingly, we found that the long-term treatment with NaCl, but not H 2O 2, affected the responses of plants to additional NaCl stress, and remarkably the roots displayed enhanced responses while the leaves showed reduced responses. Furthermore, these findings suggest that plants adapt to the long-term NaClmore » stress, while H 2O 2 might be an integrator of many stresses.« less

Authors:
 [1];  [2];  [1];  [3];  [4];  [2];  [5]
  1. Zhejiang Univ., Hangzhou (China); Hangzhou Normal Univ., Hangzhou (China)
  2. Duke Univ., Durham, NC (United States)
  3. Hangzhou Normal Univ., Hangzhou (China)
  4. Hangzhou Normal Univ., Hangzhou (China); Zhejiang Sci-Tech Univ., Hangzhou (China)
  5. Zhejiang Univ., Hangzhou (China); Hangzhou Normal Univ., Hangzhou (China); Duke Univ., Durham, NC (United States)
Publication Date:
Research Org.:
Duke Univ., Durham, NC (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1478540
Alternate Identifier(s):
OSTI ID: 1483942
Grant/Contract Number:  
SC0014077
Resource Type:
Journal Article: Published Article
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Arabidopsis thaliana; calcium imaging; aequorin; calcium signaling; basal cytosolic Ca2+ level; salt stress; oxidative stress

Citation Formats

Liu, Lulu, Jiang, Zhonghao, Zhang, Shu, Zhao, Hongyan, Yang, Weiguang, Siedow, James N., and Pei, Zhen -Ming. Both NaCl and H2O2 Long-Term Stresses Affect Basal Cytosolic Ca2+ Levels but Only NaCl Alters Cytosolic Ca2+ Signatures in Arabidopsis. United States: N. p., 2018. Web. doi:10.3389/fpls.2018.01390.
Liu, Lulu, Jiang, Zhonghao, Zhang, Shu, Zhao, Hongyan, Yang, Weiguang, Siedow, James N., & Pei, Zhen -Ming. Both NaCl and H2O2 Long-Term Stresses Affect Basal Cytosolic Ca2+ Levels but Only NaCl Alters Cytosolic Ca2+ Signatures in Arabidopsis. United States. doi:10.3389/fpls.2018.01390.
Liu, Lulu, Jiang, Zhonghao, Zhang, Shu, Zhao, Hongyan, Yang, Weiguang, Siedow, James N., and Pei, Zhen -Ming. Tue . "Both NaCl and H2O2 Long-Term Stresses Affect Basal Cytosolic Ca2+ Levels but Only NaCl Alters Cytosolic Ca2+ Signatures in Arabidopsis". United States. doi:10.3389/fpls.2018.01390.
@article{osti_1478540,
title = {Both NaCl and H2O2 Long-Term Stresses Affect Basal Cytosolic Ca2+ Levels but Only NaCl Alters Cytosolic Ca2+ Signatures in Arabidopsis},
author = {Liu, Lulu and Jiang, Zhonghao and Zhang, Shu and Zhao, Hongyan and Yang, Weiguang and Siedow, James N. and Pei, Zhen -Ming},
abstractNote = {Salinity is one of the formidable environmental factors that affect plant growth and development and constrain agricultural productivity. Experimentally imposed short-term NaCl treatment triggers a transient increase in cytosolic free Ca2+ concentration ([Ca2+]i) via Ca2+ influx across the plasma membrane. Salinity stress, as well as other stresses, induces the production of reactive oxygen species (ROS), such as H2O2. It is well established that short-term H2O2 treatment also triggers a transient increase in [Ca2+]i. However, whether and how long-term NaCl and H2O2 treatments affect the basal levels of [Ca2+]i as well as plant responses to additional NaCl and H2O2 stresses remain poorly understood. Using an aequorin-based Ca2+ imaging assay, we found that the long-term treatment of Arabidopsis seedlings with both moderate NaCl and H2O2 in the growth media reduced the basal [Ca2+]i levels. Interestingly, we found that the long-term treatment with NaCl, but not H2O2, affected the responses of plants to additional NaCl stress, and remarkably the roots displayed enhanced responses while the leaves showed reduced responses. Furthermore, these findings suggest that plants adapt to the long-term NaCl stress, while H2O2 might be an integrator of many stresses.},
doi = {10.3389/fpls.2018.01390},
journal = {Frontiers in Plant Science},
issn = {1664-462X},
number = ,
volume = 9,
place = {United States},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.3389/fpls.2018.01390

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