A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids

Ebert, Berit; Rautengarten, Carsten; McFarlane, Heather E.; Rupasinghe, Thusitha; Zeng, Wei; Ford, Kristina; Scheller, Henrik V.; Bacic, Antony; Roessner, Ute; Persson, Staffan; Heazlewood, Joshua L.

doi:10.1038/s41477-018-0235-5

A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids

Journal Article · 17 September 2018 · Nature Plants

DOI:https://doi.org/10.1038/s41477-018-0235-5· OSTI ID:1478344

Ebert, Berit; Rautengarten, Carsten; McFarlane, Heather E.; Rupasinghe, Thusitha; Zeng, Wei; Ford, Kristina; Scheller, Henrik V.; Bacic, Antony; Roessner, Ute; Persson, Staffan;

Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis. Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters. We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-d-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1). Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex and hybrid N-glycans. Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi. Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter. By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles. Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1478344

Journal Information:: Nature Plants, Vol. 4, Issue 10; ISSN 2055-0278

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

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