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Title: Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

Abstract

White–rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed–wood forests containing both soft– and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi–natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D–NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G–lignin rich spruce. Altogether, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [2];  [2];  [2];  [2];  [3];  [3];  [3];  [3];  [3];  [2];  [2];  [2];  [1];  [4];  [3]; ORCiD logo [4] more »; ORCiD logo [5] « less
  1. Utrecht Univ., Utrecht (The Netherlands)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  4. Univ. of Helsinki, Helsinki (Finland)
  5. Utrecht Univ., Utrecht (The Netherlands); Univ. of Helsinki, Helsinki (Finland)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1478261
Alternate Identifier(s):
OSTI ID: 1478264; OSTI ID: 1482214
Report Number(s):
PNNL-SA-135900
Journal ID: ISSN 1462-2912
Grant/Contract Number:  
824.15.023; 308284; AC02-05CH11231; AC05-76RL01830
Resource Type:
Journal Article: Published Article
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Name: Environmental Microbiology; Journal ID: ISSN 1462-2912
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Daly, Paul, López, Sara Casado, Peng, Mao, Lancefield, Christopher S., Purvine, Samuel O., Kim, Young -Mo, Zink, Erika M., Dohnalkova, Alice, Singan, Vasanth R., Lipzen, Anna, Dilworth, David, Wang, Mei, Ng, Vivian, Robinson, Errol, Orr, Galya, Baker, Scott E., Bruijnincx, Pieter C. A., Hildén, Kristiina S., Grigoriev, Igor V., Mäkelä, Miia R., and de Vries, Ronald P. Dichomitus squalens partially tailors its molecular responses to the composition of solid wood. United States: N. p., 2018. Web. doi:10.1111/1462-2920.14416.
Daly, Paul, López, Sara Casado, Peng, Mao, Lancefield, Christopher S., Purvine, Samuel O., Kim, Young -Mo, Zink, Erika M., Dohnalkova, Alice, Singan, Vasanth R., Lipzen, Anna, Dilworth, David, Wang, Mei, Ng, Vivian, Robinson, Errol, Orr, Galya, Baker, Scott E., Bruijnincx, Pieter C. A., Hildén, Kristiina S., Grigoriev, Igor V., Mäkelä, Miia R., & de Vries, Ronald P. Dichomitus squalens partially tailors its molecular responses to the composition of solid wood. United States. doi:10.1111/1462-2920.14416.
Daly, Paul, López, Sara Casado, Peng, Mao, Lancefield, Christopher S., Purvine, Samuel O., Kim, Young -Mo, Zink, Erika M., Dohnalkova, Alice, Singan, Vasanth R., Lipzen, Anna, Dilworth, David, Wang, Mei, Ng, Vivian, Robinson, Errol, Orr, Galya, Baker, Scott E., Bruijnincx, Pieter C. A., Hildén, Kristiina S., Grigoriev, Igor V., Mäkelä, Miia R., and de Vries, Ronald P. Mon . "Dichomitus squalens partially tailors its molecular responses to the composition of solid wood". United States. doi:10.1111/1462-2920.14416.
@article{osti_1478261,
title = {Dichomitus squalens partially tailors its molecular responses to the composition of solid wood},
author = {Daly, Paul and López, Sara Casado and Peng, Mao and Lancefield, Christopher S. and Purvine, Samuel O. and Kim, Young -Mo and Zink, Erika M. and Dohnalkova, Alice and Singan, Vasanth R. and Lipzen, Anna and Dilworth, David and Wang, Mei and Ng, Vivian and Robinson, Errol and Orr, Galya and Baker, Scott E. and Bruijnincx, Pieter C. A. and Hildén, Kristiina S. and Grigoriev, Igor V. and Mäkelä, Miia R. and de Vries, Ronald P.},
abstractNote = {White–rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed–wood forests containing both soft– and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi–natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D–NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G–lignin rich spruce. Altogether, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.},
doi = {10.1111/1462-2920.14416},
journal = {Environmental Microbiology},
number = ,
volume = ,
place = {United States},
year = {Mon Sep 24 00:00:00 EDT 2018},
month = {Mon Sep 24 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1111/1462-2920.14416

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Works referenced in this record:

Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids
journal, January 2004