Structural mechanism of Myb–MuvB assembly

Guiley, Keelan Z.; Iness, Audra N.; Saini, Siddharth; Tripathi, Sarvind; Lipsick, Joseph S.; Litovchick, Larisa; Rubin, Seth M.

doi:10.1073/pnas.1808136115

Title: Structural mechanism of Myb–MuvB assembly

Journal Article · Mon Sep 17 00:00:00 EDT 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1808136115· OSTI ID:1478077

Guiley, Keelan Z. ^[1]; Iness, Audra N. ^[2]; Saini, Siddharth ^[2];

^[1]; Lipsick, Joseph S. ^[3]; Litovchick, Larisa ^[2]; Rubin, Seth M. ^[1]

Univ. of California, Santa Cruz, CA (United States)
Virginia Commonwealth Univ., Richmond, VA (United States)
Stanford Univ., CA (United States)

The MuvB transcriptional regulatory complex, which controls cell-cycle-dependent gene expression, cooperates with B-Myb to activate genes required for the G2 and M phases of the cell cycle. We have identified the domain in B-Myb that is essential for the assembly of the Myb–MuvB (MMB) complex. We determined a crystal structure that reveals how this B-Myb domain binds MuvB through the adaptor protein LIN52 and the scaffold protein LIN9. Here, the structure and biochemical analysis provide an understanding of how oncogenic B-Myb is recruited to regulate genes required for cell-cycle progression, and the MMB interface presents a potential therapeutic target to inhibit cancer cell proliferation.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH)

Grant/Contract Number:: F31CA206244; R01CA128836; R01CA188571; R01CA132685; R01GM124148

OSTI ID:: 1478077

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 40; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 22 works

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A long lost key opens an ancient lock: Drosophila Myb causes a synthetic multivulval phenotype in nematodes Vorster, Paul J.; Goetsch, Paul; Wijeratne, Tilini U. Biology Open https://doi.org/10.1242/bio.051508	journal	January 2020
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59 BASIC BIOLOGICAL SCIENCES
cell cycle
transcription factor
Myb
MuvB
DREAM

Title: Structural mechanism of Myb–MuvB assembly

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