Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Swasey, Steven M.; Nicholson, Hunter C.; Copp, Stacy M.; Bogdanov, Petko; Gorovits, Alexander; Gwinn, Elisabeth G.

doi:10.1063/1.5023258

Title: Adaptation of a visible wavelength fluorescence microplate reader for discovery of near-infrared fluorescent probes

Journal Article · Tue Sep 25 00:00:00 EDT 2018 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.5023258· OSTI ID:1477657

Swasey, Steven M. ^[1]; Nicholson, Hunter C. ^[1];

^[2];

^[3]; Gorovits, Alexander ^[3];

^[1]

Univ. of California, Santa Barbara, CA (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
University at Albany, SUNY, Albany, NY (United States)

In this paper, we present an inexpensive, generalizable approach for modifying visible wavelength fluorescence microplate readers to detect emission in the near-infrared (NIR) I (650–950 nm) and NIR II (1000-1350 nm) tissue imaging windows. These wavelength ranges are promising for high sensitivity fluorescence-based cell assays and biological imaging, but the inaccessibility of NIR microplate readers is limiting development of the requisite, biocompatible fluorescent probes. Our modifications enable rapid screening of NIR candidate probes, using short pulses of UV light to provide excitation of diverse systems including dye molecules, semiconductor quantum dots, and metal clusters. To confirm the utility of our approach for rapid discovery of new NIR probes, we examine the silver cluster synthesis products formed on 375 candidate DNA strands that were originally designed to produce green-emitting, DNA-stabilized silver clusters. The fast, sensitive system developed here discovered DNA strands that unexpectedly stabilize NIR-emitting silver clusters.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1477657

Alternate ID(s):: OSTI ID: 1473743

Report Number(s):: LA-UR-18-21199

Journal Information:: Review of Scientific Instruments, Vol. 89, Issue 9; ISSN 0034-6748

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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High throughput near infrared screening discovers DNA-templated silver clusters with peak fluorescence beyond 950 nm Swasey, Steven M.; Copp, Stacy M.; Nicholson, Hunter C. Nanoscale, Vol. 10, Issue 42 https://doi.org/10.1039/c8nr05781h	journal	January 2018