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Synthetic methylotrophy to liquid fuels and chemicals

Patent ·
OSTI ID:1477456
; ; ; ; ; ; ; ;
A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.
Research Organization:
University of Delaware, Newark, DE (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AR0000432;
Assignee:
University of Delaware (Newark, DE)
Patent Number(s):
10,059,920
Application Number:
15/112,364
OSTI ID:
1477456
Country of Publication:
United States
Language:
English

References (11)

Growth dynamics of a methylotroph (Methylomonas L3) in continuous cultures. I. Fast transients induced by methanol pulses and methanol accumulation journal January 1987
Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase journal May 2007
Production of bio-oil and biochar from soapstock via microwave-assisted co-catalytic fast pyrolysis journal February 2017
Engineering Escherichia coli for methanol conversion journal March 2015
Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol journal May 2017
The colorimetric estimation of formaldehyde by means of the Hantzsch reaction journal October 1953
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products journal May 2000
Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism journal January 2015
Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid journal October 2003
Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus journal February 2004
Upregulated Transcription of Plasmid and Chromosomal Ribulose Monophosphate Pathway Genes Is Critical for Methanol Assimilation Rate and Methanol Tolerance in the Methylotrophic Bacterium Bacillus methanolicus journal April 2006

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