Potential for Methanosarcina to Contribute to Uranium Reduction during Acetate-Promoted Groundwater Bioremediation

Holmes, Dawn E.; Orelana, Roberto; Giloteaux, Ludovic; Wang, Li-Ying; Shrestha, Pravin; Williams, Kenneth; Lovley, Derek R.; Rotaru, Amelia-Elena

doi:10.1007/s00248-018-1165-5

Title: Potential for Methanosarcina to Contribute to Uranium Reduction during Acetate-Promoted Groundwater Bioremediation

Journal Article · Fri Mar 02 00:00:00 EST 2018 · Microbial Ecology

DOI:https://doi.org/10.1007/s00248-018-1165-5· OSTI ID:1477395

Holmes, Dawn E. ^[1]; Orelana, Roberto ^[2]; Giloteaux, Ludovic ^[3]; Wang, Li-Ying ^[2]; Shrestha, Pravin ^[4]; Williams, Kenneth ^[5]; Lovley, Derek R. ^[2]; Rotaru, Amelia-Elena ^[6]

Western New Engalnd Univ., Springfield, MA (United States); Univ. of Massachusetts, Amherst, MA (United States)
Univ. of Massachusetts, Amherst, MA (United States)
Cornell Univ., Ithaca, NY (United States)
Univ. of California, Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Southern Denmark, Odense (Denmark)

Previous studies of acetate-promoted bioremediation of uranium-contaminated aquifers focused on Geobacter because no other microorganisms that can couple the oxidation of acetate with U(VI) reduction had been detected in situ. Monitoring the levels of methyl CoM reductase subunit A (mcrA) transcripts during an acetate-injection field experiment demonstrated that acetoclastic methanogens from the genus Methanosarcina were enriched after 40 days of acetate amendment. The increased abundance of Methanosarcina corresponded with an accumulation of methane in the groundwater. In order to determine whether Methanosarcina species could be participating in U(VI) reduction in the subsurface, cell suspensions of Methanosarcina barkeri were incubated in the presence of U(VI) with acetate provided as the electron donor. U(VI) was reduced by metabolically active M. barkeri cells; however, no U(VI) reduction was observed in inactive controls. These results demonstrate that Methanosarcina species could play an important role in the long-term bioremediation of uranium-contaminated aquifers after depletion of Fe(III) oxides limits the growth of Geobacter species. The results also suggest that Methanosarcina have the potential to influence uranium geochemistry in a diversity of anaerobic sedimentary environments.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1477395

Journal Information:: Microbial Ecology, Vol. 76, Issue 3; ISSN 0095-3628

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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