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Title: Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment

Abstract

Hydrogen sulfide production by sulfate reducing bacteria (SRB) is the primary cause of oil reservoir souring. Amending environments with chlorate or perchlorate [collectively denoted (per)chlorate] represents an emerging technology to prevent the onset of souring. Recent studies with perchlorate reducing bacteria (PRB) monocultures demonstrated that they have the innate capability to enzymatically oxidize sulfide, thus PRB may offer an effective means of reversing souring. (Per)chlorate may be effective by (i) direct toxicity to SRB; (ii) competitive exclusion of SRB by PRB; or (iii) reversal of souring through re-oxidation of sulfide by PRB. To determine if (per)chlorate could sweeten a soured column system and assign a quantitative value to each of the mechanisms we treated columns flooded with San Francisco bay water with temporally decreasing amounts (50, 25, and 12.5 mM) of (per)chlorate. Geochemistry and the microbial community structure were monitored and a reactive transport model was developed, Results were compared to columns treated with nitrate or untreated. Souring was reversed by all treatments at 50 mM but nitrate-treated columns began to re-sour when treatment concentrations decreased (25 mM). Re-souring was only observed in (per)chlorate-treated columns when concentrations were decreased to 12.5 mM and the extent of re-souring was less thanmore » the control columns. Microbial community analyses indicated treatment-specific community shifts. Nitrate treatment resulted in a distinct community enriched in genera known to perform sulfur cycling metabolisms and genera capable of nitrate reduction. (Per)chlorate treatment enriched for (per)chlorate reducing bacteria. (Per)chlorate treatments only enriched for sulfate reducing organisms when treatment levels were decreased. A reactive transport model of perchlorate treatment was developed and a baseline case simulation demonstrated that the model provided a good fit to the effluent geochemical data. Subsequent simulations teased out the relative role that each of the three perchlorate inhibition mechanisms played during different phases of the experiment. These results indicate that perchlorate addition is an effective strategy for both souring prevention and souring reversal. It provides insight into which organisms are involved, and illuminates the interactive effects of the inhibition mechanisms, further highlighting the versatility of perchlorate as a sweetening agent.« less

Authors:
 [1];  [2];  [2];  [1];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [3]
  1. Univ. of California, Berkeley, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1477345
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal Issue: JUL; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Engelbrektson, Anna L., Cheng, Yiwei, Hubbard, Christopher G., Jin, Yong T., Arora, Bhavna, Tom, Lauren M., Hu, Ping, Grauel, Anna-Lena, Conrad, Mark E., Andersen, Gary L., Ajo-Franklin, Jonathan B., and Coates, John D. Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.01575.
Engelbrektson, Anna L., Cheng, Yiwei, Hubbard, Christopher G., Jin, Yong T., Arora, Bhavna, Tom, Lauren M., Hu, Ping, Grauel, Anna-Lena, Conrad, Mark E., Andersen, Gary L., Ajo-Franklin, Jonathan B., & Coates, John D. Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment. United States. doi:10.3389/fmicb.2018.01575.
Engelbrektson, Anna L., Cheng, Yiwei, Hubbard, Christopher G., Jin, Yong T., Arora, Bhavna, Tom, Lauren M., Hu, Ping, Grauel, Anna-Lena, Conrad, Mark E., Andersen, Gary L., Ajo-Franklin, Jonathan B., and Coates, John D. Thu . "Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment". United States. doi:10.3389/fmicb.2018.01575. https://www.osti.gov/servlets/purl/1477345.
@article{osti_1477345,
title = {Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment},
author = {Engelbrektson, Anna L. and Cheng, Yiwei and Hubbard, Christopher G. and Jin, Yong T. and Arora, Bhavna and Tom, Lauren M. and Hu, Ping and Grauel, Anna-Lena and Conrad, Mark E. and Andersen, Gary L. and Ajo-Franklin, Jonathan B. and Coates, John D.},
abstractNote = {Hydrogen sulfide production by sulfate reducing bacteria (SRB) is the primary cause of oil reservoir souring. Amending environments with chlorate or perchlorate [collectively denoted (per)chlorate] represents an emerging technology to prevent the onset of souring. Recent studies with perchlorate reducing bacteria (PRB) monocultures demonstrated that they have the innate capability to enzymatically oxidize sulfide, thus PRB may offer an effective means of reversing souring. (Per)chlorate may be effective by (i) direct toxicity to SRB; (ii) competitive exclusion of SRB by PRB; or (iii) reversal of souring through re-oxidation of sulfide by PRB. To determine if (per)chlorate could sweeten a soured column system and assign a quantitative value to each of the mechanisms we treated columns flooded with San Francisco bay water with temporally decreasing amounts (50, 25, and 12.5 mM) of (per)chlorate. Geochemistry and the microbial community structure were monitored and a reactive transport model was developed, Results were compared to columns treated with nitrate or untreated. Souring was reversed by all treatments at 50 mM but nitrate-treated columns began to re-sour when treatment concentrations decreased (25 mM). Re-souring was only observed in (per)chlorate-treated columns when concentrations were decreased to 12.5 mM and the extent of re-souring was less than the control columns. Microbial community analyses indicated treatment-specific community shifts. Nitrate treatment resulted in a distinct community enriched in genera known to perform sulfur cycling metabolisms and genera capable of nitrate reduction. (Per)chlorate treatment enriched for (per)chlorate reducing bacteria. (Per)chlorate treatments only enriched for sulfate reducing organisms when treatment levels were decreased. A reactive transport model of perchlorate treatment was developed and a baseline case simulation demonstrated that the model provided a good fit to the effluent geochemical data. Subsequent simulations teased out the relative role that each of the three perchlorate inhibition mechanisms played during different phases of the experiment. These results indicate that perchlorate addition is an effective strategy for both souring prevention and souring reversal. It provides insight into which organisms are involved, and illuminates the interactive effects of the inhibition mechanisms, further highlighting the versatility of perchlorate as a sweetening agent.},
doi = {10.3389/fmicb.2018.01575},
journal = {Frontiers in Microbiology},
number = JUL,
volume = 9,
place = {United States},
year = {Thu Jul 26 00:00:00 EDT 2018},
month = {Thu Jul 26 00:00:00 EDT 2018}
}

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Works referenced in this record:

Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969