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Title: Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment

Abstract

Hydrogen sulfide production by sulfate reducing bacteria (SRB) is the primary cause of oil reservoir souring. Amending environments with chlorate or perchlorate [collectively denoted (per)chlorate] represents an emerging technology to prevent the onset of souring. Recent studies with perchlorate reducing bacteria (PRB) monocultures demonstrated that they have the innate capability to enzymatically oxidize sulfide, thus PRB may offer an effective means of reversing souring. (Per)chlorate may be effective by (i) direct toxicity to SRB; (ii) competitive exclusion of SRB by PRB; or (iii) reversal of souring through re-oxidation of sulfide by PRB. To determine if (per)chlorate could sweeten a soured column system and assign a quantitative value to each of the mechanisms we treated columns flooded with San Francisco bay water with temporally decreasing amounts (50, 25, and 12.5 mM) of (per)chlorate. Geochemistry and the microbial community structure were monitored and a reactive transport model was developed, Results were compared to columns treated with nitrate or untreated. Souring was reversed by all treatments at 50 mM but nitrate-treated columns began to re-sour when treatment concentrations decreased (25 mM). Re-souring was only observed in (per)chlorate-treated columns when concentrations were decreased to 12.5 mM and the extent of re-souring was less thanmore » the control columns. Microbial community analyses indicated treatment-specific community shifts. Nitrate treatment resulted in a distinct community enriched in genera known to perform sulfur cycling metabolisms and genera capable of nitrate reduction. (Per)chlorate treatment enriched for (per)chlorate reducing bacteria. (Per)chlorate treatments only enriched for sulfate reducing organisms when treatment levels were decreased. A reactive transport model of perchlorate treatment was developed and a baseline case simulation demonstrated that the model provided a good fit to the effluent geochemical data. Subsequent simulations teased out the relative role that each of the three perchlorate inhibition mechanisms played during different phases of the experiment. These results indicate that perchlorate addition is an effective strategy for both souring prevention and souring reversal. It provides insight into which organisms are involved, and illuminates the interactive effects of the inhibition mechanisms, further highlighting the versatility of perchlorate as a sweetening agent.« less

Authors:
 [1];  [2];  [2];  [1];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [3]
  1. Univ. of California, Berkeley, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1477345
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal Issue: JUL; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Engelbrektson, Anna L., Cheng, Yiwei, Hubbard, Christopher G., Jin, Yong T., Arora, Bhavna, Tom, Lauren M., Hu, Ping, Grauel, Anna-Lena, Conrad, Mark E., Andersen, Gary L., Ajo-Franklin, Jonathan B., and Coates, John D. Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.01575.
Engelbrektson, Anna L., Cheng, Yiwei, Hubbard, Christopher G., Jin, Yong T., Arora, Bhavna, Tom, Lauren M., Hu, Ping, Grauel, Anna-Lena, Conrad, Mark E., Andersen, Gary L., Ajo-Franklin, Jonathan B., & Coates, John D. Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment. United States. doi:10.3389/fmicb.2018.01575.
Engelbrektson, Anna L., Cheng, Yiwei, Hubbard, Christopher G., Jin, Yong T., Arora, Bhavna, Tom, Lauren M., Hu, Ping, Grauel, Anna-Lena, Conrad, Mark E., Andersen, Gary L., Ajo-Franklin, Jonathan B., and Coates, John D. Thu . "Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment". United States. doi:10.3389/fmicb.2018.01575. https://www.osti.gov/servlets/purl/1477345.
@article{osti_1477345,
title = {Attenuating Sulfidogenesis in a Soured Continuous Flow Column System With Perchlorate Treatment},
author = {Engelbrektson, Anna L. and Cheng, Yiwei and Hubbard, Christopher G. and Jin, Yong T. and Arora, Bhavna and Tom, Lauren M. and Hu, Ping and Grauel, Anna-Lena and Conrad, Mark E. and Andersen, Gary L. and Ajo-Franklin, Jonathan B. and Coates, John D.},
abstractNote = {Hydrogen sulfide production by sulfate reducing bacteria (SRB) is the primary cause of oil reservoir souring. Amending environments with chlorate or perchlorate [collectively denoted (per)chlorate] represents an emerging technology to prevent the onset of souring. Recent studies with perchlorate reducing bacteria (PRB) monocultures demonstrated that they have the innate capability to enzymatically oxidize sulfide, thus PRB may offer an effective means of reversing souring. (Per)chlorate may be effective by (i) direct toxicity to SRB; (ii) competitive exclusion of SRB by PRB; or (iii) reversal of souring through re-oxidation of sulfide by PRB. To determine if (per)chlorate could sweeten a soured column system and assign a quantitative value to each of the mechanisms we treated columns flooded with San Francisco bay water with temporally decreasing amounts (50, 25, and 12.5 mM) of (per)chlorate. Geochemistry and the microbial community structure were monitored and a reactive transport model was developed, Results were compared to columns treated with nitrate or untreated. Souring was reversed by all treatments at 50 mM but nitrate-treated columns began to re-sour when treatment concentrations decreased (25 mM). Re-souring was only observed in (per)chlorate-treated columns when concentrations were decreased to 12.5 mM and the extent of re-souring was less than the control columns. Microbial community analyses indicated treatment-specific community shifts. Nitrate treatment resulted in a distinct community enriched in genera known to perform sulfur cycling metabolisms and genera capable of nitrate reduction. (Per)chlorate treatment enriched for (per)chlorate reducing bacteria. (Per)chlorate treatments only enriched for sulfate reducing organisms when treatment levels were decreased. A reactive transport model of perchlorate treatment was developed and a baseline case simulation demonstrated that the model provided a good fit to the effluent geochemical data. Subsequent simulations teased out the relative role that each of the three perchlorate inhibition mechanisms played during different phases of the experiment. These results indicate that perchlorate addition is an effective strategy for both souring prevention and souring reversal. It provides insight into which organisms are involved, and illuminates the interactive effects of the inhibition mechanisms, further highlighting the versatility of perchlorate as a sweetening agent.},
doi = {10.3389/fmicb.2018.01575},
journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = JUL,
volume = 9,
place = {United States},
year = {2018},
month = {7}
}

Works referenced in this record:

Nitrite Reactivity with Magnetite
journal, May 2013

  • Dhakal, P.; Matocha, C. J.; Huggins, F. E.
  • Environmental Science & Technology, Vol. 47, Issue 12
  • DOI: 10.1021/es304011w

Genome-resolved metagenomics identifies genetic mobility, metabolic interactions, and unexpected diversity in perchlorate-reducing communities
journal, February 2018

  • Barnum, Tyler P.; Figueroa, Israel A.; Carlström, Charlotte I.
  • The ISME Journal, Vol. 12, Issue 6
  • DOI: 10.1038/s41396-018-0081-5

A new value for the stable oxygen isotope fractionation between dissolved sulfate ion and water
journal, February 2010


Kinetics of Perchlorate- and Chlorate-Respiring Bacteria
journal, June 2001


Determination and application of the equilibrium oxygen isotope effect between water and sulfite
journal, January 2014

  • Wankel, Scott D.; Bradley, Alexander S.; Eldridge, Daniel L.
  • Geochimica et Cosmochimica Acta, Vol. 125
  • DOI: 10.1016/j.gca.2013.08.039

The Presence of Sulphate Reducing Bacteria in oil Field Waters
journal, January 1926


Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used for perchlorate reduction by Azospira sp.
journal, June 2005


Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969


A large column analog experiment of stable isotope variations during reactive transport: I. A comprehensive model of sulfur cycling and δ34S fractionation
journal, January 2014

  • Druhan, Jennifer L.; Steefel, Carl I.; Conrad, Mark E.
  • Geochimica et Cosmochimica Acta, Vol. 124
  • DOI: 10.1016/j.gca.2013.08.037

Corrosion risk associated with microbial souring control using nitrate or nitrite
journal, February 2005

  • Hubert, Casey; Nemati, Mehdi; Jenneman, Gary
  • Applied Microbiology and Biotechnology, Vol. 68, Issue 2
  • DOI: 10.1007/s00253-005-1897-2

Isotopic insights into microbial sulfur cycling in oil reservoirs
journal, September 2014

  • Hubbard, Christopher G.; Cheng, Yiwei; Engelbrekston, Anna
  • Frontiers in Microbiology, Vol. 5
  • DOI: 10.3389/fmicb.2014.00480

Microbiological Fractionation of Sulphur Isotopes
journal, February 1964


16S ribosomal DNA amplification for phylogenetic study.
journal, January 1991


Inhibition of microbial sulfate reduction in a flow-through column system by (per)chlorate treatment
journal, June 2014

  • Engelbrektson, Anna; Hubbard, Christopher G.; Tom, Lauren M.
  • Frontiers in Microbiology, Vol. 5
  • DOI: 10.3389/fmicb.2014.00315

Nitrite stability influenced by iron compounds
journal, January 1983


Oxygen isotope exchange between sulphate and water during bacterial reduction of sulphate
journal, August 1989

  • Fritz, P.; Basharmal, G. M.; Drimmie, R. J.
  • Chemical Geology: Isotope Geoscience section, Vol. 79, Issue 2
  • DOI: 10.1016/0168-9622(89)90012-2

A revised isotope fractionation model for dissimilatory sulfate reduction in sulfate reducing bacteria
journal, October 2005

  • Brunner, Benjamin; Bernasconi, Stefano M.
  • Geochimica et Cosmochimica Acta, Vol. 69, Issue 20
  • DOI: 10.1016/j.gca.2005.04.015

Mineral Transformation and Biomass Accumulation Associated With Uranium Bioremediation at Rifle, Colorado
journal, July 2009

  • Li, Li; Steefel, Carl I.; Williams, Kenneth H.
  • Environmental Science & Technology, Vol. 43, Issue 14
  • DOI: 10.1021/es900016v

Structure and Evolution of Chlorate Reduction Composite Transposons
journal, August 2013


Microbial communities acclimate to recurring changes in soil redox potential status: Fluctuating redox microbial communities
journal, December 2010


Cenozoic evolution of the sulfur cycle: Insight from oxygen isotopes in marine sulfate
journal, January 2006

  • Turchyn, Alexandra V.; Schrag, Daniel P.
  • Earth and Planetary Science Letters, Vol. 241, Issue 3-4
  • DOI: 10.1016/j.epsl.2005.11.007

Biogenic sulfide control by nitrate and (per)chlorate – A monitoring and modeling investigation
journal, January 2018


Calcium isotope fractionation in groundwater: Molecular scale processes influencing field scale behavior
journal, October 2013

  • Druhan, Jennifer L.; Steefel, Carl I.; Williams, Kenneth H.
  • Geochimica et Cosmochimica Acta, Vol. 119
  • DOI: 10.1016/j.gca.2013.05.022

Non-parametric multivariate analyses of changes in community structure
journal, March 1993


Aqueous geochemistry and oxygen isotope compositions of acid mine drainage from the Río Tinto, SW Spain, highlight inconsistencies in current models
journal, July 2009


Fluid-Rock Interaction: A Reactive Transport Approach
journal, January 2009

  • Steefel, C. I.; Maher, K.
  • Reviews in Mineralogy and Geochemistry, Vol. 70, Issue 1
  • DOI: 10.2138/rmg.2009.70.11

Oil Field Souring Control by Nitrate-Reducing Sulfurospirillum spp. That Outcompete Sulfate-Reducing Bacteria for Organic Electron Donors
journal, February 2007

  • Hubert, C.; Voordouw, G.
  • Applied and Environmental Microbiology, Vol. 73, Issue 8
  • DOI: 10.1128/AEM.02332-06

The reversibility of dissimilatory sulphate reduction and the cell-internal multi-step reduction of sulphite to sulphide: insights from the oxygen isotope composition of sulphate
journal, March 2012

  • Brunner, Benjamin; Einsiedl, Florian; Arnold, Gail L.
  • Isotopes in Environmental and Health Studies, Vol. 48, Issue 1
  • DOI: 10.1080/10256016.2011.608128

Physiological and Genetic Description of Dissimilatory Perchlorate Reduction by the Novel Marine Bacterium Arcobacter sp. Strain CAB
journal, May 2013

  • Carlström, Charlotte I.; Wang, Ouwei; Melnyk, Ryan A.
  • mBio, Vol. 4, Issue 3
  • DOI: 10.1128/mBio.00217-13

Microbial perchlorate reduction: rocket-fuelled metabolism
journal, July 2004

  • Coates, John D.; Achenbach, Laurie A.
  • Nature Reviews Microbiology, Vol. 2, Issue 7
  • DOI: 10.1038/nrmicro926

Reactive Transport Model of Sulfur Cycling as Impacted by Perchlorate and Nitrate Treatments
journal, June 2016

  • Cheng, Yiwei; Hubbard, Christopher G.; Li, Li
  • Environmental Science & Technology, Vol. 50, Issue 13
  • DOI: 10.1021/acs.est.6b00081

A model for oxygen and sulfur isotope fractionation in sulfate during bacterial sulfate reduction processes
journal, October 2005

  • Brunner, Benjamin; Bernasconi, Stefano M.; Kleikemper, Jutta
  • Geochimica et Cosmochimica Acta, Vol. 69, Issue 20
  • DOI: 10.1016/j.gca.2005.04.017

Microbial perchlorate reduction with elemental sulfur and other inorganic electron donors
journal, March 2008


Genetic dissection of chlorate respiration in P seudomonas stutzeri  PDA reveals syntrophic (per)chlorate reduction : Symbiotic (per)chlorate respiration
journal, December 2015

  • Clark, Iain C.; Youngblut, Matt; Jacobsen, Gillian
  • Environmental Microbiology, Vol. 18, Issue 10
  • DOI: 10.1111/1462-2920.13068

Inhibition of microbial H 2 S production in an oil reservoir model column by nitrate injection
journal, March 2002


Dissimilatory perchlorate reduction: A review
journal, May 2011


Elucidating microbial processes in nitrate- and sulfate-reducing systems using sulfur and oxygen isotope ratios: The example of oil reservoir souring control
journal, July 2009

  • Hubert, Casey; Voordouw, Gerrit; Mayer, Bernhard
  • Geochimica et Cosmochimica Acta, Vol. 73, Issue 13
  • DOI: 10.1016/j.gca.2009.03.025

Competitive and Non-competitive Inhibitors of Bacterial Sulphate Reduction
journal, February 1952


Testing of null hypotheses in exploratory community analyses: similarity profiles and biota-environment linkage
journal, November 2008

  • Clarke, K. Robert; Somerfield, Paul J.; Gorley, Raymond N.
  • Journal of Experimental Marine Biology and Ecology, Vol. 366, Issue 1-2
  • DOI: 10.1016/j.jembe.2008.07.009

Control of sulfidogenesis through bio-oxidation of H 2 S coupled to (per)chlorate reduction : (Per)chlorate-mediated sulfide bio-oxidation
journal, April 2014

  • Gregoire, Patrick; Engelbrektson, Anna; Hubbard, Christopher G.
  • Environmental Microbiology Reports, Vol. 6, Issue 6
  • DOI: 10.1111/1758-2229.12156

Mechanisms of direct inhibition of the respiratory sulfate-reduction pathway by (per)chlorate and nitrate
journal, November 2014

  • Carlson, Hans K.; Kuehl, Jennifer V.; Hazra, Amrita B.
  • The ISME Journal, Vol. 9, Issue 6
  • DOI: 10.1038/ismej.2014.216

The oxygen isotope equilibrium fractionation between sulfite species and water
journal, November 2013

  • Müller, Inigo A.; Brunner, Benjamin; Breuer, Christian
  • Geochimica et Cosmochimica Acta, Vol. 120
  • DOI: 10.1016/j.gca.2013.06.037

Biological souring and mitigation in oil reservoirs
journal, August 2011

  • Gieg, Lisa M.; Jack, Tom R.; Foght, Julia M.
  • Applied Microbiology and Biotechnology, Vol. 92, Issue 2
  • DOI: 10.1007/s00253-011-3542-6

Nitrite reductase activity of sulphate-reducing bacteria prevents their inhibition by nitrate-reducing, sulphide-oxidizing bacteria
journal, July 2003


Modeling Coupled Chemical and Isotopic Equilibration Rates
journal, January 2014


Chlorate — a potent inhibitor of protein sulfation in intact cells
journal, December 1986

  • Baeuerle, Patrick A.; Huttner, Wieland B.
  • Biochemical and Biophysical Research Communications, Vol. 141, Issue 2
  • DOI: 10.1016/S0006-291X(86)80253-4

Timing the Onset of Sulfate Reduction over Multiple Subsurface Acetate Amendments by Measurement and Modeling of Sulfur Isotope Fractionation
journal, August 2012

  • Druhan, Jennifer L.; Steefel, Carl I.; Molins, Sergi
  • Environmental Science & Technology, Vol. 46, Issue 16
  • DOI: 10.1021/es302016p

Mechanism of H 2 S Oxidation by the Dissimilatory Perchlorate-Reducing Microorganism Azospira suillum PS
journal, February 2017


Chemolithotrophic perchlorate reduction linked to the oxidation of elemental sulfur
journal, January 2007

  • Ju, Xiumin; Field, Jim A.; Sierra-Alvarez, Reyes
  • Biotechnology and Bioengineering, Vol. 96, Issue 6
  • DOI: 10.1002/bit.21197

Sulfide Remediation by Pulsed Injection of Nitrate into a Low Temperature Canadian Heavy Oil Reservoir
journal, December 2009

  • Voordouw, Gerrit; Grigoryan, Aleksandr A.; Lambo, Adewale
  • Environmental Science & Technology, Vol. 43, Issue 24
  • DOI: 10.1021/es902211j

Deep-Sea Oil Plume Enriches Indigenous Oil-Degrading Bacteria
journal, August 2010


Temperature and injection water source influence microbial community structure in four Alaskan North Slope hydrocarbon reservoirs
journal, August 2014