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Title: Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose

Abstract

Protein glycosylation pathways have been identified in a variety of bacteria and are best understood in pathogens and commensals in which the glycosylation targets are cell surface proteins, such as S layers, pili, and flagella. In contrast, very little is known about the glycosylation of bacterial enzymes, especially those secreted by cellulolytic bacteria. Caldicellulosiruptor bescii secretes several unique synergistic multifunctional biomass-degrading enzymes, notably cellulase A which is largely responsible for this organism's ability to grow on lignocellulosic biomass without the conventional pretreatment. It was recently discovered that extracellular CelA is heavily glycosylated. In this work, we identified an O-glycosyltransferase in the C. bescii chromosome and targeted it for deletion. The resulting mutant was unable to grow on crystalline cellulose and showed no detectable protein glycosylation. Multifunctional biomass-degrading enzymes in this strain were rapidly degraded. Furthermore with the genetic tools available in C. bescii, this system represents a unique opportunity to study the role of bacterial enzyme glycosylation as well an investigation of the pathway for protein glycosylation in a non-pathogen.

Authors:
 [1];  [2];  [3];  [4];  [5];  [5];  [3];  [2]
  1. Univ. of Georgia, Athens, GA (United States); U.S. Dept. of Energy Office of Science, Oak Ridge, TN (United States)
  2. Univ. of Georgia, Athens, GA (United States); Chung-Ang Univ., Gyeonggi (Republic of Korea); U.S. Dept. of Energy Office of Science, Oak Ridge, TN (United States)
  3. Univ. of Georgia, Athens, GA (United States)
  4. Univ. of Alberta, Edmonton, AB (Canada)
  5. National Renewable Energy Lab. (NREL), Golden, CO (United States); U.S. Dept. of Energy Office of Science, Oak Ridge, TN (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1476874
Report Number(s):
NREL/JA-2700-72558
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bacterial enzymes; glycosylation; lignocellulosic biomass

Citation Formats

Russell, Jordan, Kim, Sun -Ki, Duma, Justin, Nothaft, Harald, Himmel, Michael E., Bomble, Yannick J., Szymanski, Christine M., and Westpheling, Janet. Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose. United States: N. p., 2018. Web. doi:10.1186/s13068-018-1266-x.
Russell, Jordan, Kim, Sun -Ki, Duma, Justin, Nothaft, Harald, Himmel, Michael E., Bomble, Yannick J., Szymanski, Christine M., & Westpheling, Janet. Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose. United States. doi:10.1186/s13068-018-1266-x.
Russell, Jordan, Kim, Sun -Ki, Duma, Justin, Nothaft, Harald, Himmel, Michael E., Bomble, Yannick J., Szymanski, Christine M., and Westpheling, Janet. Mon . "Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose". United States. doi:10.1186/s13068-018-1266-x. https://www.osti.gov/servlets/purl/1476874.
@article{osti_1476874,
title = {Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose},
author = {Russell, Jordan and Kim, Sun -Ki and Duma, Justin and Nothaft, Harald and Himmel, Michael E. and Bomble, Yannick J. and Szymanski, Christine M. and Westpheling, Janet},
abstractNote = {Protein glycosylation pathways have been identified in a variety of bacteria and are best understood in pathogens and commensals in which the glycosylation targets are cell surface proteins, such as S layers, pili, and flagella. In contrast, very little is known about the glycosylation of bacterial enzymes, especially those secreted by cellulolytic bacteria. Caldicellulosiruptor bescii secretes several unique synergistic multifunctional biomass-degrading enzymes, notably cellulase A which is largely responsible for this organism's ability to grow on lignocellulosic biomass without the conventional pretreatment. It was recently discovered that extracellular CelA is heavily glycosylated. In this work, we identified an O-glycosyltransferase in the C. bescii chromosome and targeted it for deletion. The resulting mutant was unable to grow on crystalline cellulose and showed no detectable protein glycosylation. Multifunctional biomass-degrading enzymes in this strain were rapidly degraded. Furthermore with the genetic tools available in C. bescii, this system represents a unique opportunity to study the role of bacterial enzyme glycosylation as well an investigation of the pathway for protein glycosylation in a non-pathogen.},
doi = {10.1186/s13068-018-1266-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {United States},
year = {Mon Sep 24 00:00:00 EDT 2018},
month = {Mon Sep 24 00:00:00 EDT 2018}
}

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Works referenced in this record:

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