Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater

Ribicic, Deni; Netzer, Roman; Hazen, Terry C.; Techtmann, Stephen M.; Drabløs, Finn; Brakstad, Odd Gunnar

doi:10.1016/j.marpolbul.2018.02.034

Title: Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater

Journal Article · Fri Mar 23 00:00:00 EDT 2018 · Marine Pollution Bulletin

DOI:https://doi.org/10.1016/j.marpolbul.2018.02.034· OSTI ID:1476403

^[1]; Netzer, Roman ^[2]; Hazen, Terry C. ^[3]; Techtmann, Stephen M. ^[4]; Drabløs, Finn ^[1]; Brakstad, Odd Gunnar ^[2]

Norwegian Univ. of Science and Technology, Trondheim (Norway)
SINTEF OCEAN, Trondheim (Norway)
Univ. of Tennessee, Knoxville, TN (United States)
Michigan Technological Univ., Houghton, MI (United States)

Oil biodegradation as a weathering process has been extensively investigated over the years, especially after the Deepwater Horizon blowout. In this study, we performed microcosm experiments at 5 °C with chemically dispersed oil in non-amended seawater. We link biodegradation processes with microbial community and metagenome dynamics and explain the succession based on substrate specialization. Reconstructed genomes and 16S rRNA gene analysis revealed that Bermanella and Zhongshania were the main contributors to initial n-alkane breakdown, while subsequent abundances of Colwellia and microorganisms closely related to Porticoccaceae were involved in secondary n-alkane breakdown and beta-oxidation. Cycloclasticus, Porticoccaceae and Spongiiabcteraceae were associated with degradation of mono- and poly-cyclic aromatics. Successional pattern of genes coding for hydrocarbon degrading enzymes at metagenome level, and reconstructed genomic content, revealed a high differentiation of bacteria involved in hydrocarbon biodegradation. A cooperation among oil degrading microorganisms is thus needed for the complete substrate transformation.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1476403

Alternate ID(s):: OSTI ID: 1479397

Journal Information:: Marine Pollution Bulletin, Vol. 129, Issue 1; ISSN 0025-326X

Country of Publication:: United States

Language:: English

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Cited by: 51 works

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