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Title: Design and simulation of a snapshot multi-focal interferometric microscope

Abstract

Realizing both high temporal and spatial resolution across a large volume is a key challenge for 3D fluorescent imaging. Towards achieving this objective, we introduce an interferometric multifocus microscopy (iMFM) system, a combination of multifocus microscopy (MFM) with two opposing objective lenses. We show that the proposed iMFM is capable of simultaneously producing multiple focal plane interferometry that provides axial super-resolution and hence isotropic 3D resolution with a single exposure. We design and simulate the iMFM microscope by employing two special diffractive optical elements. The point spread function of this new iMFM microscope is simulated and the image formation model is given. For reconstruction, we use the Richardson-Lucy deconvolution algorithm with total variation regularization for 3D extended object recovery, and a maximum likelihood estimator (MLE) for single molecule tracking. A method for determining an initial axial position of the molecule is also proposed to improve the convergence of the MLE. We demonstrate both theoretically and numerically that isotropic 3D nanoscopic localization accuracy is achievable with an axial imaging range of 2um when tracking a fluorescent molecule in three dimensions and that the diffraction limited axial resolution can be improved by 3-4 times in the single shot wide-field 3D extended objectmore » recovery. We believe that iMFM will be a useful tool in 3D dynamic event imaging that requires both high temporal and spatial resolution.« less

Authors:
 [1];  [2];  [3];  [1];  [1]; ORCiD logo [3];  [2];  [3];  [2];  [1];  [1]
  1. Northwestern Univ., Evanston, IL (United States)
  2. Argonne National Lab. (ANL), Lemont, IL (United States)
  3. Univ. of Chicago, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1476375
Alternate Identifier(s):
OSTI ID: 1488542
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Published Article
Journal Name:
Optics Express
Additional Journal Information:
Journal Volume: 26; Journal Issue: 21; Journal ID: ISSN 1094-4087
Publisher:
Optical Society of America (OSA)
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING; 36 MATERIALS SCIENCE

Citation Formats

He, Kuan, Huang, Xiang, Wang, Xiaolei, Yoo, Seunghwan, Ruiz, Pablo, Gdor, Itay, Ferrier, Nicola J., Scherer, Norbert, Hereld, Mark, Katsaggelos, Aggelos K., and Cossairt, Oliver. Design and simulation of a snapshot multi-focal interferometric microscope. United States: N. p., 2018. Web. doi:10.1364/OE.26.027381.
He, Kuan, Huang, Xiang, Wang, Xiaolei, Yoo, Seunghwan, Ruiz, Pablo, Gdor, Itay, Ferrier, Nicola J., Scherer, Norbert, Hereld, Mark, Katsaggelos, Aggelos K., & Cossairt, Oliver. Design and simulation of a snapshot multi-focal interferometric microscope. United States. doi:10.1364/OE.26.027381.
He, Kuan, Huang, Xiang, Wang, Xiaolei, Yoo, Seunghwan, Ruiz, Pablo, Gdor, Itay, Ferrier, Nicola J., Scherer, Norbert, Hereld, Mark, Katsaggelos, Aggelos K., and Cossairt, Oliver. Fri . "Design and simulation of a snapshot multi-focal interferometric microscope". United States. doi:10.1364/OE.26.027381.
@article{osti_1476375,
title = {Design and simulation of a snapshot multi-focal interferometric microscope},
author = {He, Kuan and Huang, Xiang and Wang, Xiaolei and Yoo, Seunghwan and Ruiz, Pablo and Gdor, Itay and Ferrier, Nicola J. and Scherer, Norbert and Hereld, Mark and Katsaggelos, Aggelos K. and Cossairt, Oliver},
abstractNote = {Realizing both high temporal and spatial resolution across a large volume is a key challenge for 3D fluorescent imaging. Towards achieving this objective, we introduce an interferometric multifocus microscopy (iMFM) system, a combination of multifocus microscopy (MFM) with two opposing objective lenses. We show that the proposed iMFM is capable of simultaneously producing multiple focal plane interferometry that provides axial super-resolution and hence isotropic 3D resolution with a single exposure. We design and simulate the iMFM microscope by employing two special diffractive optical elements. The point spread function of this new iMFM microscope is simulated and the image formation model is given. For reconstruction, we use the Richardson-Lucy deconvolution algorithm with total variation regularization for 3D extended object recovery, and a maximum likelihood estimator (MLE) for single molecule tracking. A method for determining an initial axial position of the molecule is also proposed to improve the convergence of the MLE. We demonstrate both theoretically and numerically that isotropic 3D nanoscopic localization accuracy is achievable with an axial imaging range of 2um when tracking a fluorescent molecule in three dimensions and that the diffraction limited axial resolution can be improved by 3-4 times in the single shot wide-field 3D extended object recovery. We believe that iMFM will be a useful tool in 3D dynamic event imaging that requires both high temporal and spatial resolution.},
doi = {10.1364/OE.26.027381},
journal = {Optics Express},
issn = {1094-4087},
number = 21,
volume = 26,
place = {United States},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1364/OE.26.027381

Citation Metrics:
Cited by: 1 work
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Figures / Tables:

Fig. 1. Fig. 1.: Comparison between (a) I2M +MFM and (b) the proposed iMFM systems. Both systems consist of two opposing objectives, one beam splitter (BS) and one detector. However, in (a), an MFG is placed in the Fourier plane behind BS, prohibiting the correct interference to occur on the detector. Inmore » (b), two MFGs of opposite focal shifts are employed in the respective Fourier planes of dual objectives before BS, and are capable of producing multifocal interferometry detection on the BS, which is then imaged via a 4f system (lenses L3 and L4) onto the detector in a single exposure.« less

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Works referenced in this record:

Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure
journal, February 2009

  • Shtengel, G.; Galbraith, J. A.; Galbraith, C. G.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 9, p. 3125-3130
  • DOI: 10.1073/pnas.0813131106