Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling

Stromberg, Loreen R.; Mendez, Heather M.; Kubicek-Sutherland, Jessica Z.; Graves, Steven W.; Hengartner, Nicolas W.; Mukundan, Harshini; Bernardino de la Serna, ed., Jorge

doi:10.1371/journal.pone.0198531

Title: Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling

Journal Article · Thu Jun 14 00:00:00 EDT 2018 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0198531· OSTI ID:1454322

Stromberg, Loreen R.; Mendez, Heather M.;

; Graves, Steven W.; Hengartner, Nicolas W.;

; Bernardino de la Serna, ed., Jorge

Recognition of Pathogen-associated Molecular Patterns (PAMPs) by Toll-like receptors is central to innate immunity. Many bacterial PAMPs such as lipopolysaccharide (LPS) and lipoteichoic acid have amphiphilic properties. The hydrophobicity of amphiphilic PAMPs contributes to increasing entropy and causes these molecules to self-aggregate or bind host carrier proteins in aqueous physiological environments. The goal of this work was to determine how innate immune signaling is impacted by physical presentation and association of amphiphilic PAMPs with serum carrier proteins, using LPS as an example molecule. Specifically, we measured LPS-induced cytokine profiles in murine macrophages when the antigen was presented associated with the various serum carrier proteins in serum versus a serum-depleted system. Our study demonstrates that the observed cytokine profiles are dramatically different when LPS is presented in buffer, versus in serum when it is associated with proteins, specifically with respect to inhibition of pro-inflammatory cytokines in the latter. These studies suggest that LPS-mediated cytokine expression is dependent on its presentation in physiological systems. The amphiphilicity of bacterial PAMPs and consequent association with lipoproteins is a feature, which should be taken into account in the design of in vitro experiments. Further studies of the interdependencies of different serum carriers can identify pathways for drug delivery and diagnostics.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); LANL Laboratory Directed Research and Development (LDRD) Program; USDA National Inst. of Food and Agriculture (NIFA)

Grant/Contract Number:: AC52-06NA25396; 2012-68003-30155

OSTI ID:: 1454322

Alternate ID(s):: OSTI ID: 1475350

Report Number(s):: LA-UR-17-27524; 10.1371/journal.pone.0198531

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Vol. 13 Journal Issue: 6; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 7 works

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