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Title: Using sound pulses to solve the crystal-harvesting bottleneck

Abstract

Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ -1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.

Authors:
 [1];  [2];  [3];  [4];  [3];  [3];  [3];  [5];  [6];  [6];  [6]; ORCiD logo [7]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States); Univ. Federal de Santa Maria, Santa Maria-RS (Brazil)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States); College of William and Mary, Williamsburg, VA (United States)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States); Stony Brook Univ., Stony Brook, NY (United States)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States); York College of Pennsylvania, York, PA (United States)
  5. Brookhaven National Lab. (BNL), Upton, NY (United States); Georgia Inst. of Technology, Atlanta, GA (United States)
  6. Brookhaven National Lab. (BNL), Upton, NY (United States)
  7. Univ. Federal de Minas Gerais, Belo Horizonte-MG (Brazil)
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1475235
Alternate Identifier(s):
OSTI ID: 1483750
Report Number(s):
BNL-209517-2018-JAAM
Journal ID: ISSN 2059-7983; ACSDAD
Grant/Contract Number:  
SC0012704; KP1605010
Resource Type:
Journal Article: Published Article
Journal Name:
Acta Crystallographica. Section D. Structural Biology
Additional Journal Information:
Journal Volume: 74; Journal Issue: 10; Journal ID: ISSN 2059-7983
Publisher:
IUCr
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; crystal harvesting; acoustic droplet ejection; crystal mounting; automation; crystallography; microcrystals; high-throughput screening; drug discovery

Citation Formats

Samara, Yasmin N., Brennan, Haley M., McCarthy, Liam, Bollard, Mary T., Laspina, Denise, Wlodek, Jakub M., Campos, Stefanie L., Natarajan, Ramya, Gofron, Kazimierz, McSweeney, Sean, Soares, Alexei S., and Leroy, Ludmila. Using sound pulses to solve the crystal-harvesting bottleneck. United States: N. p., 2018. Web. doi:10.1107/S2059798318011506.
Samara, Yasmin N., Brennan, Haley M., McCarthy, Liam, Bollard, Mary T., Laspina, Denise, Wlodek, Jakub M., Campos, Stefanie L., Natarajan, Ramya, Gofron, Kazimierz, McSweeney, Sean, Soares, Alexei S., & Leroy, Ludmila. Using sound pulses to solve the crystal-harvesting bottleneck. United States. doi:10.1107/S2059798318011506.
Samara, Yasmin N., Brennan, Haley M., McCarthy, Liam, Bollard, Mary T., Laspina, Denise, Wlodek, Jakub M., Campos, Stefanie L., Natarajan, Ramya, Gofron, Kazimierz, McSweeney, Sean, Soares, Alexei S., and Leroy, Ludmila. Tue . "Using sound pulses to solve the crystal-harvesting bottleneck". United States. doi:10.1107/S2059798318011506.
@article{osti_1475235,
title = {Using sound pulses to solve the crystal-harvesting bottleneck},
author = {Samara, Yasmin N. and Brennan, Haley M. and McCarthy, Liam and Bollard, Mary T. and Laspina, Denise and Wlodek, Jakub M. and Campos, Stefanie L. and Natarajan, Ramya and Gofron, Kazimierz and McSweeney, Sean and Soares, Alexei S. and Leroy, Ludmila},
abstractNote = {Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ -1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.},
doi = {10.1107/S2059798318011506},
journal = {Acta Crystallographica. Section D. Structural Biology},
number = 10,
volume = 74,
place = {United States},
year = {Tue Oct 02 00:00:00 EDT 2018},
month = {Tue Oct 02 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1107/S2059798318011506

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Works referenced in this record:

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