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Title: Cell type-resolved human lung lipidome reveals cellular cooperation in lung function

Abstract

Cell type-resolved proteome analyses of the brain, heart and liver have been reported, however a similar effort on the lipidome is currently lacking. Here we applied liquid chromatography-tandem mass spectrometry to characterize the lipidome of major lung cell types isolated from human donors, representing the first lipidome map of any organ. We coupled this with cell type-resolved proteomics of the same samples (available at Lungmap.net). Complementary proteomics analyses substantiated the functional identity of the isolated cells. Lipidomics analyses showed significant variations in the lipidome across major human lung cell types, with differences most evident at the subclass and intra-subclass (i.e. total carbon length of the fatty acid chains) level. Further, lipidomic signatures revealed an overarching posture of high cellular cooperation within the human lung to support critical functions. In conclusion, our complementary cell type-resolved lipid and protein datasets serve as a rich resource for analyses of human lung function.

Authors:
 [1];  [1];  [2];  [2];  [1];  [1];  [1];  [3];  [2]; ORCiD logo [2];  [2];  [2];  [2];  [4];  [1];  [5];  [2];  [3];  [3];  [2] more »;  [1] « less
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Rochester School of Medicine and Dentistry, Rochester, NY (United States)
  3. Cincinnati Children's Hospital Medical Center, Cincinnati, OH (United States)
  4. Seattle Children's Hospital, Seattle, WA (United States)
  5. Univ. of Texas at Austin, Austin, TX (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1475201
Report Number(s):
PNNL-SA-132404
Journal ID: ISSN 2045-2322; PII: 31640
Grant/Contract Number:  
HL122703; HL122700; HL122642; HL122638; AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Kyle, Jennifer E., Clair, Geremy, Bandyopadhyay, Gautam, Misra, Ravi S., Zink, Erika M., Bloodsworth, Kent J., Shukla, Anil K., Du, Yina, Lillis, Jacquelyn, Myers, Jason R., Ashton, John, Bushnell, Timothy, Cochran, Matthew, Deutsch, Gail, Baker, Erin S., Carson, James P., Mariani, Thomas J., Xu, Yan, Whitsett, Jeffrey A., Pryhuber, Gloria, and Ansong, Charles. Cell type-resolved human lung lipidome reveals cellular cooperation in lung function. United States: N. p., 2018. Web. doi:10.1038/S41598-018-31640-X.
Kyle, Jennifer E., Clair, Geremy, Bandyopadhyay, Gautam, Misra, Ravi S., Zink, Erika M., Bloodsworth, Kent J., Shukla, Anil K., Du, Yina, Lillis, Jacquelyn, Myers, Jason R., Ashton, John, Bushnell, Timothy, Cochran, Matthew, Deutsch, Gail, Baker, Erin S., Carson, James P., Mariani, Thomas J., Xu, Yan, Whitsett, Jeffrey A., Pryhuber, Gloria, & Ansong, Charles. Cell type-resolved human lung lipidome reveals cellular cooperation in lung function. United States. doi:10.1038/S41598-018-31640-X.
Kyle, Jennifer E., Clair, Geremy, Bandyopadhyay, Gautam, Misra, Ravi S., Zink, Erika M., Bloodsworth, Kent J., Shukla, Anil K., Du, Yina, Lillis, Jacquelyn, Myers, Jason R., Ashton, John, Bushnell, Timothy, Cochran, Matthew, Deutsch, Gail, Baker, Erin S., Carson, James P., Mariani, Thomas J., Xu, Yan, Whitsett, Jeffrey A., Pryhuber, Gloria, and Ansong, Charles. Fri . "Cell type-resolved human lung lipidome reveals cellular cooperation in lung function". United States. doi:10.1038/S41598-018-31640-X. https://www.osti.gov/servlets/purl/1475201.
@article{osti_1475201,
title = {Cell type-resolved human lung lipidome reveals cellular cooperation in lung function},
author = {Kyle, Jennifer E. and Clair, Geremy and Bandyopadhyay, Gautam and Misra, Ravi S. and Zink, Erika M. and Bloodsworth, Kent J. and Shukla, Anil K. and Du, Yina and Lillis, Jacquelyn and Myers, Jason R. and Ashton, John and Bushnell, Timothy and Cochran, Matthew and Deutsch, Gail and Baker, Erin S. and Carson, James P. and Mariani, Thomas J. and Xu, Yan and Whitsett, Jeffrey A. and Pryhuber, Gloria and Ansong, Charles},
abstractNote = {Cell type-resolved proteome analyses of the brain, heart and liver have been reported, however a similar effort on the lipidome is currently lacking. Here we applied liquid chromatography-tandem mass spectrometry to characterize the lipidome of major lung cell types isolated from human donors, representing the first lipidome map of any organ. We coupled this with cell type-resolved proteomics of the same samples (available at Lungmap.net). Complementary proteomics analyses substantiated the functional identity of the isolated cells. Lipidomics analyses showed significant variations in the lipidome across major human lung cell types, with differences most evident at the subclass and intra-subclass (i.e. total carbon length of the fatty acid chains) level. Further, lipidomic signatures revealed an overarching posture of high cellular cooperation within the human lung to support critical functions. In conclusion, our complementary cell type-resolved lipid and protein datasets serve as a rich resource for analyses of human lung function.},
doi = {10.1038/S41598-018-31640-X},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {Fri Sep 07 00:00:00 EDT 2018},
month = {Fri Sep 07 00:00:00 EDT 2018}
}

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Works referenced in this record:

Conformational Ordering of Biomolecules in the Gas Phase: Nitrogen Collision Cross Sections Measured on a Prototype High Resolution Drift Tube Ion Mobility-Mass Spectrometer
journal, February 2014

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