Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase

Tian, Liang; Perot, Skyler J.; Hon, Shuen; Zhou, Jilai; Liang, Xiaoyu; Bouvier, Jason T.; Guss, Adam M.; Olson, Daniel G.; Lynd, Lee R.

doi:10.1186/s12934-017-0783-9

Title: Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase

Journal Article · Wed Oct 04 00:00:00 EDT 2017 · Microbial Cell Factories

DOI:https://doi.org/10.1186/s12934-017-0783-9· OSTI ID:1618893

; Perot, Skyler J.; Hon, Shuen; Zhou, Jilai; Liang, Xiaoyu; Bouvier, Jason T.; Guss, Adam M.; Olson, Daniel G.; Lynd, Lee R.

Here, pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Here we examined the thermostability of eight PDCs. The purified bacterial enzymes retained 20% of activity after incubation for 30 min at 55 °C. Expression of these PDC genes, except the one from Zymomonas mobilis, improved ethanol production by Clostridium thermocellum. Ethanol production was further improved by expression of the heterologous alcohol dehydrogenase gene adhA from Thermoanaerobacterium saccharolyticum. Finally, the best PDC enzyme was from Acetobactor pasteurianus. A strain of C. thermocellum expressing the pdc gene from A. pasteurianus and the adhA gene from T. saccharolyticum was able to produce 21.3 g/L ethanol from 60 g/L cellulose, which is 70% of the theoretical maximum yield.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1618893

Alternate ID(s):: OSTI ID: 1474857

Journal Information:: Microbial Cell Factories, Journal Name: Microbial Cell Factories Vol. 16 Journal Issue: 1; ISSN 1475-2859

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 26 works

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59 BASIC BIOLOGICAL SCIENCES
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Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase [Supplemental Data] Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/M9.FIGSHARE.C.3897049 The current record is supplemented by this collection	collection	October 2017
Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase [Supplemental Data] Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049.v1 The current record is supplemented by this collection	collection	October 2017
MOESM10 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/M9.FIGSHARE.C.3897049_D1 The current record is supplemented by this dataset	dataset	October 2017
MOESM10 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d1.v1 The current record is supplemented by this dataset	dataset	October 2017
MOESM7 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d10 The current record is supplemented by this dataset	dataset	October 2017
MOESM7 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d10.v1 The current record is supplemented by this dataset	dataset	October 2017
MOESM8 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/M9.FIGSHARE.C.3897049_D11 The current record is supplemented by this dataset	dataset	October 2017
MOESM8 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d11.v1 The current record is supplemented by this dataset	dataset	October 2017
MOESM9 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d12 The current record is supplemented by this dataset	dataset	October 2017
MOESM9 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d12.v1 The current record is supplemented by this dataset	dataset	October 2017
MOESM11 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/M9.FIGSHARE.C.3897049_D2 The current record is supplemented by this dataset	dataset	October 2017
MOESM12 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/M9.FIGSHARE.C.3897049_D3 The current record is supplemented by this dataset	dataset	October 2017
MOESM12 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d3.v1 The current record is supplemented by this dataset	dataset	October 2017
MOESM1 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d4 The current record is supplemented by this figure	figure	October 2017
MOESM2 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d5 The current record is supplemented by this figure	figure	October 2017
MOESM2 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d5.v1 The current record is supplemented by this figure	figure	October 2017
MOESM3 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d6 The current record is supplemented by this dataset	dataset	October 2017
MOESM3 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d6.v1 The current record is supplemented by this dataset	dataset	October 2017
MOESM4 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d7 The current record is supplemented by this dataset	dataset	October 2017
MOESM5 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d8 The current record is supplemented by this figure	figure	October 2017
MOESM5 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d8.v1 The current record is supplemented by this figure	figure	October 2017
MOESM6 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d9 The current record is supplemented by this dataset	dataset	October 2017
MOESM6 of Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase Tian, Liang; Perot, Skyler; Bouvier, Jason https://doi.org/10.6084/m9.figshare.c.3897049_d9.v1 The current record is supplemented by this dataset	dataset	October 2017