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Title: Mechanisms of subzero growth in the cryophile Planococcus halocryophilus determined through proteomic analysis

Abstract

The eurypsychrophilic bacterium Planococcus halocryophilus is capable of growth down to -15°C, making it ideal for studying adaptations to subzero growth. In this paper, to increase our understanding of the mechanisms and pathways important for subzero growth, we performed proteomics on P. halocryophilus grown at 23°C, 23°C with 12% w/v NaCl and -10°C with 12% w/v NaCl. Many proteins with increased abundances at -10°C versus 23°C also increased at 23C-salt versus 23°C, indicating a closely tied relationship between salt and cold stress adaptation. Processes which displayed the largest changes in protein abundance were peptidoglycan and fatty acid (FA) synthesis, translation processes, methylglyoxal metabolism, DNA repair and recombination, and protein and nucleotide turnover. We identified intriguing targets for further research at -10°C, including PlsX and KASII (FA metabolism), DD-transpeptidase and MurB (peptidoglycan synthesis), glyoxalase family proteins (reactive electrophile response) and ribosome modifying enzymes (translation turnover). PemK/MazF may have a crucial role in translational reprogramming under cold conditions. At -10°C P. halocryophilus induces stress responses, uses resources efficiently, and carefully controls its growth and metabolism to maximize subzero survival. Finally, the present study identifies several mechanisms involved in subzero growth and enhances our understanding of cold adaptation.

Authors:
ORCiD logo [1];  [2];  [1];  [3];  [2];  [1]
  1. McGill Univ., Sainte-Anne-de-Bellevue, QC (Canada). Macdonald Campus
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division; Univ. of Tennessee, Knoxville, TN (United States). Graduate School of Genome Science and Technology
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McGill Univ., Sainte-Anne-de-Bellevue, QC (Canada)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Natural Sciences and Engineering Research Council of Canada (NSERC)
OSTI Identifier:
1474718
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Volume: 19; Journal Issue: 11; Journal ID: ISSN 1462-2912
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Raymond-Bouchard, Isabelle, Chourey, Karuna, Altshuler, Ianina, Iyer, Ramsunder, Hettich, Robert L., and Whyte, Lyle G. Mechanisms of subzero growth in the cryophile Planococcus halocryophilus determined through proteomic analysis. United States: N. p., 2017. Web. doi:10.1111/1462-2920.13893.
Raymond-Bouchard, Isabelle, Chourey, Karuna, Altshuler, Ianina, Iyer, Ramsunder, Hettich, Robert L., & Whyte, Lyle G. Mechanisms of subzero growth in the cryophile Planococcus halocryophilus determined through proteomic analysis. United States. doi:10.1111/1462-2920.13893.
Raymond-Bouchard, Isabelle, Chourey, Karuna, Altshuler, Ianina, Iyer, Ramsunder, Hettich, Robert L., and Whyte, Lyle G. Wed . "Mechanisms of subzero growth in the cryophile Planococcus halocryophilus determined through proteomic analysis". United States. doi:10.1111/1462-2920.13893. https://www.osti.gov/servlets/purl/1474718.
@article{osti_1474718,
title = {Mechanisms of subzero growth in the cryophile Planococcus halocryophilus determined through proteomic analysis},
author = {Raymond-Bouchard, Isabelle and Chourey, Karuna and Altshuler, Ianina and Iyer, Ramsunder and Hettich, Robert L. and Whyte, Lyle G.},
abstractNote = {The eurypsychrophilic bacterium Planococcus halocryophilus is capable of growth down to -15°C, making it ideal for studying adaptations to subzero growth. In this paper, to increase our understanding of the mechanisms and pathways important for subzero growth, we performed proteomics on P. halocryophilus grown at 23°C, 23°C with 12% w/v NaCl and -10°C with 12% w/v NaCl. Many proteins with increased abundances at -10°C versus 23°C also increased at 23C-salt versus 23°C, indicating a closely tied relationship between salt and cold stress adaptation. Processes which displayed the largest changes in protein abundance were peptidoglycan and fatty acid (FA) synthesis, translation processes, methylglyoxal metabolism, DNA repair and recombination, and protein and nucleotide turnover. We identified intriguing targets for further research at -10°C, including PlsX and KASII (FA metabolism), DD-transpeptidase and MurB (peptidoglycan synthesis), glyoxalase family proteins (reactive electrophile response) and ribosome modifying enzymes (translation turnover). PemK/MazF may have a crucial role in translational reprogramming under cold conditions. At -10°C P. halocryophilus induces stress responses, uses resources efficiently, and carefully controls its growth and metabolism to maximize subzero survival. Finally, the present study identifies several mechanisms involved in subzero growth and enhances our understanding of cold adaptation.},
doi = {10.1111/1462-2920.13893},
journal = {Environmental Microbiology},
number = 11,
volume = 19,
place = {United States},
year = {Wed Aug 23 00:00:00 EDT 2017},
month = {Wed Aug 23 00:00:00 EDT 2017}
}

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Works referenced in this record:

Thermal regulation of membrane lipid fluidity in bacteria
journal, February 1983