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Title: Monolignol ferulate conjugates are naturally incorporated into plant lignins

Abstract

Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl–coenzyme A monolignol transferase, OsFMT1 (AT5), in rice and its orthologs in other monocots.

Authors:
 [1];  [2];  [2];  [1];  [3];  [1];  [4];  [5];  [6];  [1];  [7];  [8];  [9];  [10];  [11];  [12];  [2];  [1]
  1. Univ. of Wisconsin, Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center, and Dept. of Biochemistry
  2. Univ. of Oklahoma, Norman, OK (United States). Dept. of Microbiology and Plant Biology
  3. Univ. of Wisconsin, Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center
  4. Univ. of Auckland, Auckland (New Zealand). School of Biological Sciences, and School of Chemical Sciences
  5. Univ. of Florida, Wimauma, FL (United States). Dept. of Horticultural Science, Inst. of Food and Agricultural Sciences (IFAS) Gulf Coast Research and Education Center
  6. Univ. of Auckland, Auckland (New Zealand). School of Chemical Sciences
  7. Illinois State Univ., Normal, IL (United States). Dept. of Energy Great Lakes Bioenergy Research Center, School of Biological Sciences
  8. Inst. National de la Recherche Agronomique and AgroParisTech, Inst. Jean-Pierre Bourgin UMR, Versailles (France)
  9. U.S. Dept. of Agriculture, Madison, WI (United States). U.S. Dairy Forage Research Center, Agricultural Research Service
  10. Univ. of Wisconsin, Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center, and Dept. of Biological Systems Engineering
  11. Samuel Roberts Noble Foundation, Ardmore, OK (United States). Plant Biology Division
  12. Univ. of Auckland, Auckland (New Zealand). School of Biological Sciences
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1473881
Grant/Contract Number:  
FC02-07ER64494; AI02-06ER64299
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Science Advances
Additional Journal Information:
Journal Volume: 2; Journal Issue: 10; Journal ID: ISSN 2375-2548
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Grasses; Rice; Monolignol; Lignin; DFRC; GC-MS; Phylogenetic tree; BAHD transferase; Transgenic; Momocot

Citation Formats

Karlen, Steven D., Zhang, Chengcheng, Peck, Matthew L., Smith, Rebecca A., Padmakshan, Dharshana, Helmich, Kate E., Free, Heather C. A., Lee, Seonghee, Smith, Bronwen G., Lu, F., Sedbrook, J. C., Sibout, Richard, Grabber, John H., Runge, Troy M., Mysore, Kirankumar S., Harris, Philip J., Bartley, Laura E., and Ralph, John. Monolignol ferulate conjugates are naturally incorporated into plant lignins. United States: N. p., 2016. Web. doi:10.1126/sciadv.1600393.
Karlen, Steven D., Zhang, Chengcheng, Peck, Matthew L., Smith, Rebecca A., Padmakshan, Dharshana, Helmich, Kate E., Free, Heather C. A., Lee, Seonghee, Smith, Bronwen G., Lu, F., Sedbrook, J. C., Sibout, Richard, Grabber, John H., Runge, Troy M., Mysore, Kirankumar S., Harris, Philip J., Bartley, Laura E., & Ralph, John. Monolignol ferulate conjugates are naturally incorporated into plant lignins. United States. doi:10.1126/sciadv.1600393.
Karlen, Steven D., Zhang, Chengcheng, Peck, Matthew L., Smith, Rebecca A., Padmakshan, Dharshana, Helmich, Kate E., Free, Heather C. A., Lee, Seonghee, Smith, Bronwen G., Lu, F., Sedbrook, J. C., Sibout, Richard, Grabber, John H., Runge, Troy M., Mysore, Kirankumar S., Harris, Philip J., Bartley, Laura E., and Ralph, John. Fri . "Monolignol ferulate conjugates are naturally incorporated into plant lignins". United States. doi:10.1126/sciadv.1600393. https://www.osti.gov/servlets/purl/1473881.
@article{osti_1473881,
title = {Monolignol ferulate conjugates are naturally incorporated into plant lignins},
author = {Karlen, Steven D. and Zhang, Chengcheng and Peck, Matthew L. and Smith, Rebecca A. and Padmakshan, Dharshana and Helmich, Kate E. and Free, Heather C. A. and Lee, Seonghee and Smith, Bronwen G. and Lu, F. and Sedbrook, J. C. and Sibout, Richard and Grabber, John H. and Runge, Troy M. and Mysore, Kirankumar S. and Harris, Philip J. and Bartley, Laura E. and Ralph, John},
abstractNote = {Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl–coenzyme A monolignol transferase, OsFMT1 (AT5), in rice and its orthologs in other monocots.},
doi = {10.1126/sciadv.1600393},
journal = {Science Advances},
issn = {2375-2548},
number = 10,
volume = 2,
place = {United States},
year = {2016},
month = {10}
}

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Cited by: 45 works
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    Works referencing / citing this record:

    Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility
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    • de Souza, Wagner Rodrigo; Pacheco, Thályta Fraga; Duarte, Karoline Estefani
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