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Title: Production of single cell protein from agro-waste using Rhodococcus opacus

Abstract

Here, livestock and fish farming are rapidly growing industries facing the simultaneous pressure of increasing production demands and limited protein required to produce feed. Bacteria that can convert low-value non-food waste streams into singe cell protein (SCP) present an intriguing route for rapid protein production. The oleaginous bacterium Rhodococcus opacus serves as a model organism for understanding microbial lipid production. SCP production has not been explored using an organism from this genus. In the present research, R. opacus strains DSM 1069 and PD630 were fed three agro-waste streams: (1) orange pulp, juice, and peel; (2) lemon pulp, juice, and peel; and (3) corn stover effluent, to determine if these low-cost substrates would be suitable for producing a value-added product, SCP for aquafarming or livestock feed. Both strains used agro-waste carbon sources as a growth substrate to produce protein-rich cell biomass suggesting that that R. opacus can be used to produce SCP using agro-wastes as low-cost substrates.

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [2];  [3];  [3];  [3]; ORCiD logo [4]
  1. Univ. of Tennessee, Knoxville, TN (United States)
  2. Texas A & M Univ., College Station, TX (United States)
  3. Michigan State Univ., East Lansing, MI (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1471892
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 45; Journal Issue: 9; Journal ID: ISSN 1367-5435
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Agro-waste; Citrus waste; Corn stover; Rhodococcus opacus; Single cell protein

Citation Formats

Mahan, Kristina M., Le, Rosemary K., Wells, Jr., Tyrone, Anderson, Seth, Yuan, Joshua S., Stoklosa, Ryan J., Bhalla, Aditya, Hodge, David B., and Ragauskas, Arthur J.. Production of single cell protein from agro-waste using Rhodococcus opacus. United States: N. p., 2018. Web. doi:10.1007/s10295-018-2043-3.
Mahan, Kristina M., Le, Rosemary K., Wells, Jr., Tyrone, Anderson, Seth, Yuan, Joshua S., Stoklosa, Ryan J., Bhalla, Aditya, Hodge, David B., & Ragauskas, Arthur J.. Production of single cell protein from agro-waste using Rhodococcus opacus. United States. doi:10.1007/s10295-018-2043-3.
Mahan, Kristina M., Le, Rosemary K., Wells, Jr., Tyrone, Anderson, Seth, Yuan, Joshua S., Stoklosa, Ryan J., Bhalla, Aditya, Hodge, David B., and Ragauskas, Arthur J.. Mon . "Production of single cell protein from agro-waste using Rhodococcus opacus". United States. doi:10.1007/s10295-018-2043-3.
@article{osti_1471892,
title = {Production of single cell protein from agro-waste using Rhodococcus opacus},
author = {Mahan, Kristina M. and Le, Rosemary K. and Wells, Jr., Tyrone and Anderson, Seth and Yuan, Joshua S. and Stoklosa, Ryan J. and Bhalla, Aditya and Hodge, David B. and Ragauskas, Arthur J.},
abstractNote = {Here, livestock and fish farming are rapidly growing industries facing the simultaneous pressure of increasing production demands and limited protein required to produce feed. Bacteria that can convert low-value non-food waste streams into singe cell protein (SCP) present an intriguing route for rapid protein production. The oleaginous bacterium Rhodococcus opacus serves as a model organism for understanding microbial lipid production. SCP production has not been explored using an organism from this genus. In the present research, R. opacus strains DSM 1069 and PD630 were fed three agro-waste streams: (1) orange pulp, juice, and peel; (2) lemon pulp, juice, and peel; and (3) corn stover effluent, to determine if these low-cost substrates would be suitable for producing a value-added product, SCP for aquafarming or livestock feed. Both strains used agro-waste carbon sources as a growth substrate to produce protein-rich cell biomass suggesting that that R. opacus can be used to produce SCP using agro-wastes as low-cost substrates.},
doi = {10.1007/s10295-018-2043-3},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = 9,
volume = 45,
place = {United States},
year = {Mon Jun 18 00:00:00 EDT 2018},
month = {Mon Jun 18 00:00:00 EDT 2018}
}

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Works referenced in this record:

The β-Ketoadipate Pathway And The Biology Of Self-Identity
journal, October 1996