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Title: Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells

Abstract

Plant cell walls contribute the majority of plant biomass that can be used to produce transportation fuels. However, the complexity and variability in composition and structure of cell walls, particularly the presence of lignin, negatively impacts their deconstruction for bioenergy. Metabolic and genetic changes associated with secondary wall development in the biofuel crop switchgrass (Panicum virgatum) have yet to be reported. Our previous studies have established a cell suspension system for switchgrass, in which cell wall lignification can be induced by application of brassinolide (BL). We have now collected cell wall composition and microarray-based transcriptome profiles for BL-induced and non-induced suspension cultures to provide an overview of the dynamic changes in transcriptional reprogramming during BL-induced cell wall modification. From this analysis, we have identified changes in candidate genes involved in cell wall precursor synthesis, cellulose, hemicellulose, and pectin formation and ester-linkage generation. We have also identified a large number of transcription factors with expression correlated with lignin biosynthesis genes, among which are candidates for control of syringyl (S) lignin accumulation. Together, this work provides an overview of the dynamic compositional changes during brassinosteroid-induced cell wall remodeling, and identifies candidate genes for future plant genetic engineering to overcome cell wall recalcitrance.

Authors:
 [1];  [2];  [3];  [3];  [3];  [3];  [4];  [5];  [4];  [1]; ORCiD logo [1]
  1. Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst. Dept. of Biological Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
  2. Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst. Dept. of Biological Sciences
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States); Univ. of North Texas, Denton, TX (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF)
OSTI Identifier:
1471891
Grant/Contract Number:  
AC05-00OR22725; DBI-0421683; IOS-0923992
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; brassinosteroid; cell wall; comparative transcriptomics; glycome profiling; lignin; suspension cell

Citation Formats

Rao, Xiaolan, Shen, Hui, Pattathil, Sivakumar, Hahn, Michael G., Gelineo-Albersheim, Ivana, Mohnen, Debra, Pu, Yunqiao, Ragauskas, Arthur J., Chen, Xin, Chen, Fang, and Dixon, Richard A. Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0954-2.
Rao, Xiaolan, Shen, Hui, Pattathil, Sivakumar, Hahn, Michael G., Gelineo-Albersheim, Ivana, Mohnen, Debra, Pu, Yunqiao, Ragauskas, Arthur J., Chen, Xin, Chen, Fang, & Dixon, Richard A. Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells. United States. doi:10.1186/s13068-017-0954-2.
Rao, Xiaolan, Shen, Hui, Pattathil, Sivakumar, Hahn, Michael G., Gelineo-Albersheim, Ivana, Mohnen, Debra, Pu, Yunqiao, Ragauskas, Arthur J., Chen, Xin, Chen, Fang, and Dixon, Richard A. Thu . "Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells". United States. doi:10.1186/s13068-017-0954-2. https://www.osti.gov/servlets/purl/1471891.
@article{osti_1471891,
title = {Dynamic changes in transcriptome and cell wall composition underlying brassinosteroid-mediated lignification of switchgrass suspension cells},
author = {Rao, Xiaolan and Shen, Hui and Pattathil, Sivakumar and Hahn, Michael G. and Gelineo-Albersheim, Ivana and Mohnen, Debra and Pu, Yunqiao and Ragauskas, Arthur J. and Chen, Xin and Chen, Fang and Dixon, Richard A.},
abstractNote = {Plant cell walls contribute the majority of plant biomass that can be used to produce transportation fuels. However, the complexity and variability in composition and structure of cell walls, particularly the presence of lignin, negatively impacts their deconstruction for bioenergy. Metabolic and genetic changes associated with secondary wall development in the biofuel crop switchgrass (Panicum virgatum) have yet to be reported. Our previous studies have established a cell suspension system for switchgrass, in which cell wall lignification can be induced by application of brassinolide (BL). We have now collected cell wall composition and microarray-based transcriptome profiles for BL-induced and non-induced suspension cultures to provide an overview of the dynamic changes in transcriptional reprogramming during BL-induced cell wall modification. From this analysis, we have identified changes in candidate genes involved in cell wall precursor synthesis, cellulose, hemicellulose, and pectin formation and ester-linkage generation. We have also identified a large number of transcription factors with expression correlated with lignin biosynthesis genes, among which are candidates for control of syringyl (S) lignin accumulation. Together, this work provides an overview of the dynamic compositional changes during brassinosteroid-induced cell wall remodeling, and identifies candidate genes for future plant genetic engineering to overcome cell wall recalcitrance.},
doi = {10.1186/s13068-017-0954-2},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = ,
volume = 10,
place = {United States},
year = {2017},
month = {11}
}

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Figures / Tables:

Fig. 1 Fig. 1: Experimental design. Switchgrass cell suspension cultures were transferred to fresh medium containing 9 μM 2,4-D (controls, blue), or to medium with 0.9 μM 2,4-D supplemented with 0.2 μM BL (induced, orange), and harvested at the times shown. The changes in cell wall composition of induced and non-induced samplesmore » were analyzed by biochemical and immunochemical approaches at all the times shown. Microarray analysis was performed on induced samples harvested at 0, 6 h, 1, 3, and 7 days after transfer, and on non-induced samples 1 and 7 days after transfer. Comparative transcriptomics and correlation with changes in cell wall composition were applied to identify putative cell wall-related genes« less

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