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Title: Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass

Abstract

Neutron scattering of deuterated plants can provide fundamental insight into the structure of lignocellulosics in plant cell walls and its deconstruction by pretreatment and enzymes. Such plants need to be characterized for any alterations to lignocellulosic structure caused by growth in deuterated media. Here we show that glucose yields from enzymatic hydrolysis at lower enzyme loading were 35% and 30% for untreated deuterated and protiated switchgrass, respectively. Lignin content was 4% higher in deuterated switchgrass but there were no significant lignin structural differences. Transmission electron microscopy showed differences in lignin distribution and packing of fibers in the cell walls that apparently increased surface area of cellulose in deuterated switchgrass, increasing cellulose accessibility and lowering its recalcitrance. These differences in lignification were likely caused by abiotic stress due to growth in deuterated media.

Authors:
 [1];  [1];  [2];  [3];  [4];  [5];  [5];  [6];  [7];  [8];  [9]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
  3. Univ. of Tennessee, Knoxville, TN (United States). Advanced Microscopy and Imaging Center
  4. Georgia Inst. of Technology, Atlanta, GA (United States). Renewable Bioproducts Inst. School of Chemistry and Biochemistry
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Carbon and Composite Group. Materials Science and Technology Division; Univ. of Tennessee, Knoxville, TN (United States). The Bredesen Center for Interdisciplinary Research and Graduate Education
  7. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  8. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. of Biological Sciences. Biosciences Division
  9. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering. Inst. of Agriculture. Center for Renewable Carbon. Dept. of Forestry, Wildlife, and Fisheries; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. of Biological Sciences. Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1471889
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bioalcohols; biofuels

Citation Formats

Bhagia, Samarthya, Meng, Xianzhi, Evans, Barbara R., Dunlap, John R., Bali, Garima, Chen, Jihua, Reeves, Kimberly Shawn, Ho, Hoi Chun, Davison, Brian H., Pu, Yunqiao, and Ragauskas, Arthur J. Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass. United States: N. p., 2018. Web. doi:10.1038/s41598-018-31269-w.
Bhagia, Samarthya, Meng, Xianzhi, Evans, Barbara R., Dunlap, John R., Bali, Garima, Chen, Jihua, Reeves, Kimberly Shawn, Ho, Hoi Chun, Davison, Brian H., Pu, Yunqiao, & Ragauskas, Arthur J. Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass. United States. doi:10.1038/s41598-018-31269-w.
Bhagia, Samarthya, Meng, Xianzhi, Evans, Barbara R., Dunlap, John R., Bali, Garima, Chen, Jihua, Reeves, Kimberly Shawn, Ho, Hoi Chun, Davison, Brian H., Pu, Yunqiao, and Ragauskas, Arthur J. Wed . "Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass". United States. doi:10.1038/s41598-018-31269-w. https://www.osti.gov/servlets/purl/1471889.
@article{osti_1471889,
title = {Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass},
author = {Bhagia, Samarthya and Meng, Xianzhi and Evans, Barbara R. and Dunlap, John R. and Bali, Garima and Chen, Jihua and Reeves, Kimberly Shawn and Ho, Hoi Chun and Davison, Brian H. and Pu, Yunqiao and Ragauskas, Arthur J.},
abstractNote = {Neutron scattering of deuterated plants can provide fundamental insight into the structure of lignocellulosics in plant cell walls and its deconstruction by pretreatment and enzymes. Such plants need to be characterized for any alterations to lignocellulosic structure caused by growth in deuterated media. Here we show that glucose yields from enzymatic hydrolysis at lower enzyme loading were 35% and 30% for untreated deuterated and protiated switchgrass, respectively. Lignin content was 4% higher in deuterated switchgrass but there were no significant lignin structural differences. Transmission electron microscopy showed differences in lignin distribution and packing of fibers in the cell walls that apparently increased surface area of cellulose in deuterated switchgrass, increasing cellulose accessibility and lowering its recalcitrance. These differences in lignification were likely caused by abiotic stress due to growth in deuterated media.},
doi = {10.1038/s41598-018-31269-w},
journal = {Scientific Reports},
number = ,
volume = 8,
place = {United States},
year = {Wed Sep 05 00:00:00 EDT 2018},
month = {Wed Sep 05 00:00:00 EDT 2018}
}

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Works referenced in this record:

Lignification and lignin topochemistry — an ultrastructural view
journal, July 2001


Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

  • Chen, Fang; Dixon, Richard A.
  • Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
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The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
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  • DOI: 10.1126/science.1114736