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Title: Insulin hexamer dissociation dynamics revealed by photoinduced T -jumps and time-resolved X-ray solution scattering

Abstract

The structural dynamics of insulin hexamer dissociation were studied by the photoinduced temperature jump technique and monitored by time-resolved X-ray scattering.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3];  [3]; ORCiD logo [4]
  1. Department of Chemistry; Northwestern University; Evanston; USA
  2. Department of Chemistry and Biochemistry; University of Delaware; Newark; USA
  3. Center for Advanced Radiation Sources; The University of Chicago; USA
  4. Department of Chemistry; Northwestern University; Evanston; USA; Chemical Sciences and Engineering Division
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1471508
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Photochemical & Photobiological Sciences
Additional Journal Information:
Journal Volume: 17; Journal Issue: 7; Journal ID: ISSN 1474-905X
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English

Citation Formats

Rimmerman, Dolev, Leshchev, Denis, Hsu, Darren J., Hong, Jiyun, Abraham, Baxter, Kosheleva, Irina, Henning, Robert, and Chen, Lin X. Insulin hexamer dissociation dynamics revealed by photoinduced T -jumps and time-resolved X-ray solution scattering. United States: N. p., 2018. Web. doi:10.1039/c8pp00034d.
Rimmerman, Dolev, Leshchev, Denis, Hsu, Darren J., Hong, Jiyun, Abraham, Baxter, Kosheleva, Irina, Henning, Robert, & Chen, Lin X. Insulin hexamer dissociation dynamics revealed by photoinduced T -jumps and time-resolved X-ray solution scattering. United States. https://doi.org/10.1039/c8pp00034d
Rimmerman, Dolev, Leshchev, Denis, Hsu, Darren J., Hong, Jiyun, Abraham, Baxter, Kosheleva, Irina, Henning, Robert, and Chen, Lin X. 2018. "Insulin hexamer dissociation dynamics revealed by photoinduced T -jumps and time-resolved X-ray solution scattering". United States. https://doi.org/10.1039/c8pp00034d.
@article{osti_1471508,
title = {Insulin hexamer dissociation dynamics revealed by photoinduced T -jumps and time-resolved X-ray solution scattering},
author = {Rimmerman, Dolev and Leshchev, Denis and Hsu, Darren J. and Hong, Jiyun and Abraham, Baxter and Kosheleva, Irina and Henning, Robert and Chen, Lin X.},
abstractNote = {The structural dynamics of insulin hexamer dissociation were studied by the photoinduced temperature jump technique and monitored by time-resolved X-ray scattering.},
doi = {10.1039/c8pp00034d},
url = {https://www.osti.gov/biblio/1471508}, journal = {Photochemical & Photobiological Sciences},
issn = {1474-905X},
number = 7,
volume = 17,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2018},
month = {Mon Jan 01 00:00:00 EST 2018}
}

Works referenced in this record:

pH-dependent self-association of zinc-free insulin characterized by concentration-gradient static light scattering
journal, May 2010


Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor
journal, June 2017


Probing Cytochrome c Folding Transitions upon Phototriggered Environmental Perturbations Using Time-Resolved X-ray Scattering
journal, April 2018


The structure of T 6 bovine insulin
journal, October 2005


Microscopic Reversibility of Protein Folding in Molecular Dynamics Simulations of the Engrailed Homeodomain
journal, July 2008


Structure of human insulin monomer in water/acetonitrile solution
journal, November 2007


Advances in X-ray scattering: from solution SAXS to achievements with coherent beams
journal, October 2012


Application of Singular Value Decomposition to the Analysis of Time-Resolved Macromolecular X-Ray Data
journal, March 2003


The role of assembly in insulin's biosynthesis
journal, April 1998


BioCARS: a synchrotron resource for time-resolved X-ray science
journal, May 2011


Ferritin Assembly Revisited: A Time-Resolved Small-Angle X-ray Scattering Study
journal, January 2016


Conformational landscape of cytochrome c folding studied by microsecond-resolved small-angle x-ray scattering
journal, January 2002


Visualizing Solution-Phase Reaction Dynamics with Time-Resolved X-ray Liquidography
journal, February 2009


Laser Temperature Jump Induced Protein Refolding
journal, November 1998


Direct Observation of Cooperative Protein Structural Dynamics of Homodimeric Hemoglobin from 100 ps to 10 ms with Pump–Probe X-ray Solution Scattering
journal, April 2012


Insulin Loaded PLGA Microspheres: Effect of Zinc Salts on Encapsulation, Release, and Stability
journal, February 2009


Prediction of the association state of insulin using spectral parameters
journal, April 2003


Monitoring and Inhibition of Insulin Fibrillation by a Small Organic Fluorogen with Aggregation-Induced Emission Characteristics
journal, January 2012


Structural Characterisation and Functional Significance of Transient Protein–Protein Interactions
journal, January 2003


Conformational Substates of Myoglobin Intermediate Resolved by Picosecond X-ray Solution Scattering
journal, February 2014


Thermal Dissociation and Unfolding of Insulin
journal, August 2005


Picosecond Photobiology: Watching a Signaling Protein Function in Real Time via Time-Resolved Small- and Wide-Angle X-ray Scattering
journal, July 2016


Insulin dimer dissociation and unfolding revealed by amide I two-dimensional infrared spectroscopy
journal, January 2010


Transient 2D IR spectroscopy of ubiquitin unfolding dynamics
journal, June 2007


Impulsive solvent heating probed by picosecond x-ray diffraction
journal, March 2006


Anisotropic Picosecond X-ray Solution Scattering from Photoselectively Aligned Protein Molecules
journal, February 2011


Partially Folded Intermediates in Insulin Fibrillation
journal, October 2003


T HE F AST P ROTEIN F OLDING P ROBLEM
journal, October 1999


Molecular dynamics simulations of unfolding and refolding of a  -hairpin fragment of protein G
journal, August 1999


A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins
journal, August 2015


Direct Observation of Insulin Association Dynamics with Time-Resolved X-ray Scattering
journal, September 2017


Sequential conformational transitions and α-helical supercoiling regulate a sensor histidine kinase
journal, August 2017


Protein structural dynamics in solution unveiled via 100-ps time-resolved x-ray scattering
journal, April 2010


Studying Protein–Protein Binding through T-Jump Induced Dissociation: Transient 2D IR Spectroscopy of Insulin Dimer
journal, May 2016


Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering
journal, September 2008


Amyloid Fibrillation of Insulin under Water-Limited Conditions
journal, October 2014


Stimulation of Insulin Fibrillation by Urea-induced Intermediates
journal, January 2004


Protein Structural Dynamics of Photoactive Yellow Protein in Solution Revealed by Pump–Probe X-ray Solution Scattering
journal, February 2012


Protein folding and unfolding on a complex energy landscape
journal, February 2000


Multimerization and Aggregation of Native-State Insulin: Effect of Zinc
journal, December 2011


Unifying features in protein-folding mechanisms
journal, October 2003


Characterization of the nanoscale properties of individual amyloid fibrils
journal, October 2006


Time-resolved methods in biophysics. 9. Laser temperature-jump methods for investigating biomolecular dynamics
journal, January 2009


Protein Folding and Unfolding at Atomic Resolution
journal, February 2002