Single-particle imaging without symmetry constraints at an X-ray free-electron laser

Rose, Max; Bobkov, Sergey; Ayyer, Kartik; Kurta, Ruslan P.; Dzhigaev, Dmitry; Kim, Young Yong; Morgan, Andrew J.; Yoon, Chun Hong; Westphal, Daniel; Bielecki, Johan; Sellberg, Jonas A.; Williams, Garth; Maia, Filipe R. N. C.; Yefanov, Olexander M.; Ilyin, Vyacheslav; Mancuso, Adrian P.; Chapman, Henry N.; Hogue, Brenda G.; Aquila, Andrew; Barty, Anton; Vartanyants, Ivan A.

doi:10.1107/S205225251801120X

Title: Single-particle imaging without symmetry constraints at an X-ray free-electron laser

Journal Article · Tue Sep 18 00:00:00 EDT 2018 · IUCrJ

DOI:https://doi.org/10.1107/S205225251801120X· OSTI ID:1471255

Rose, Max; Bobkov, Sergey; Ayyer, Kartik; Kurta, Ruslan P.; Dzhigaev, Dmitry; Kim, Young Yong; Morgan, Andrew J.; Yoon, Chun Hong; Westphal, Daniel; Bielecki, Johan; Sellberg, Jonas A.; Williams, Garth; Maia, Filipe R. N. C.; Yefanov, Olexander M.; Ilyin, Vyacheslav; Mancuso, Adrian P.; Chapman, Henry N.; Hogue, Brenda G.; Aquila, Andrew; Barty, Anton more »

The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231; AC02-76SF00515

OSTI ID:: 1471255

Alternate ID(s):: OSTI ID: 1490704

Journal Information:: IUCrJ, Journal Name: IUCrJ Vol. 5 Journal Issue: 6; ISSN 2052-2525

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 54 works

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