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Title: Carbon conserving microbial pathway for the production of caprolactam from renewable feedstocks

Abstract

When microorganisms use biological feedstocks such as sugars or glycerol to make more reduced chemicals, deoxygenation most often occurs via the loss of the feedstock’s carbon in the form of carbon dioxide (CO 2). Feedstocks typically account for more than half of the total costs of a bioprocess, making them the most expensive component. Thus, the carbon lost as CO 2 generally precludes profitability for many bio-based endeavors. To overcome this economics challenge, a unique carbon conserving (C 2) platform technology is being developed at ZymoChem to minimize or eliminate CO 2 production when microbes convert bio-based feedstocks into chemicals. ZymoChem’s C 2 technology increases the maximum product yields by up to 50%, which could substantially decrease both capital costs and operating costs, in turn enabling the commercial-scale production of many chemicals in a sustainable, profitable manner. This Phase I SBIR project focussed on developing a novel biosynthetic pathway for producing ε-caprolactam from metabolic intermediates that can be made by microbes using ZymoChem’s C 2 technology. One of two goals during Phase I will be to validate the feasibility of the proposed ε-caprolactam pathway. The second goal of this Phase I project was to develop our core C 2 biosyntheticmore » pathway in engineered strains of E. coli (available through the Technology Transfer Opportunity) that have improved anaerobic production of pyruvate, wherein the objective will be to have these strains improved our C 2 technology, and specifically for the production of ε-caprolactam. Completing these objectives represented the first example of the microbial production of ε-caprolactam, establishing the foundation for Phase II efforts at ZymoChem that will encompass strain engineering, pathway optimization, and scale-up R&D at the pilot level.« less

Authors:
 [1]
  1. ZymoChem, Inc., Castro Valley, CA (United States)
Publication Date:
Research Org.:
ZymoChem, Inc., Castro Valley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1470766
Report Number(s):
DOE-ZymoChem-0017084
DOE Contract Number:  
SC0017084
Type / Phase:
SBIR (Phase I)
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; caprolactam

Citation Formats

Chokhawala, Harshal. Carbon conserving microbial pathway for the production of caprolactam from renewable feedstocks. United States: N. p., 2018. Web.
Chokhawala, Harshal. Carbon conserving microbial pathway for the production of caprolactam from renewable feedstocks. United States.
Chokhawala, Harshal. Fri . "Carbon conserving microbial pathway for the production of caprolactam from renewable feedstocks". United States.
@article{osti_1470766,
title = {Carbon conserving microbial pathway for the production of caprolactam from renewable feedstocks},
author = {Chokhawala, Harshal},
abstractNote = {When microorganisms use biological feedstocks such as sugars or glycerol to make more reduced chemicals, deoxygenation most often occurs via the loss of the feedstock’s carbon in the form of carbon dioxide (CO2). Feedstocks typically account for more than half of the total costs of a bioprocess, making them the most expensive component. Thus, the carbon lost as CO2 generally precludes profitability for many bio-based endeavors. To overcome this economics challenge, a unique carbon conserving (C2) platform technology is being developed at ZymoChem to minimize or eliminate CO2 production when microbes convert bio-based feedstocks into chemicals. ZymoChem’s C2 technology increases the maximum product yields by up to 50%, which could substantially decrease both capital costs and operating costs, in turn enabling the commercial-scale production of many chemicals in a sustainable, profitable manner. This Phase I SBIR project focussed on developing a novel biosynthetic pathway for producing ε-caprolactam from metabolic intermediates that can be made by microbes using ZymoChem’s C2 technology. One of two goals during Phase I will be to validate the feasibility of the proposed ε-caprolactam pathway. The second goal of this Phase I project was to develop our core C2 biosynthetic pathway in engineered strains of E. coli (available through the Technology Transfer Opportunity) that have improved anaerobic production of pyruvate, wherein the objective will be to have these strains improved our C2 technology, and specifically for the production of ε-caprolactam. Completing these objectives represented the first example of the microbial production of ε-caprolactam, establishing the foundation for Phase II efforts at ZymoChem that will encompass strain engineering, pathway optimization, and scale-up R&D at the pilot level.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {9}
}

Technical Report:
This technical report may be released as soon as September 14, 2022
Other availability
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