Methodological Guidelines for Accurate Detection of Viruses in Wild Plant Species

Lacroix, Christelle; Renner, Kurra; Cole, Ellen; Seabloom, Eric W.; Borer, Elizabeth T.; Malmstrom, Carolyn M.

doi:10.1128/AEM.03538-15

Title: Methodological Guidelines for Accurate Detection of Viruses in Wild Plant Species

Journal Article · Fri Jan 15 00:00:00 EST 2016 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.03538-15· OSTI ID:1470727

^[1]; Renner, Kurra ^[2]; Cole, Ellen ^[3]; Seabloom, Eric W. ^[2]; Borer, Elizabeth T. ^[2]; Malmstrom, Carolyn M. ^[4]

Univ. of Minnesota, Saint Paul, MN (United States). Dept of Ecology, Evolution, and Behavior; Planat Pathology, Montfavet, France (Europe)
Univ. of Minnesota, Saint Paul, MN (United States). Dept of Ecology, Evolution, and Behavior
Loyola Marymount Univ., Los Angeles, CA (United States)
Michigan State Univ., East Lansing, MI (United States)

Here ecological understanding of disease risk, emergence, and dynamics and of the efficacy of control strategies relies heavily on efficient tools for microorganism identification and characterization. Misdetection, such as the misclassification of infected hosts as healthy, can strongly bias estimates of disease prevalence and lead to inaccurate conclusions. In natural plant ecosystems, interest in assessing microbial dynamics is increasing exponentially, but guidelines for detection of microorganisms in wild plants remain limited, particularly so for plant viruses. To address this gap, we explored issues and solutions associated with virus detection by serological and molecular methods in noncrop plant species as applied to the globally important Barley yellow dwarf virus PAV (Luteoviridae), which infects wild native plants as well as crops. With enzyme-linked immunosorbent assays (ELISA), we demonstrate how virus detection in a perennial wild plant species may be much greater in stems than in leaves, although leaves are most commonly sampled, and may also vary among tillers within an individual, thereby highlighting the importance of designing effective sampling strategies. With reverse transcription-PCR (RT-PCR), we demonstrate how inhibitors in tissues of perennial wild hosts can suppress virus detection but can be overcome with methods and products that improve isolation and amplification of nucleic acids. These examples demonstrate the paramount importance of testing and validating survey designs and virus detection methods for noncrop plant communities to ensure accurate ecological surveys and reliable assumptions about virus dynamics in wild hosts.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1470727

Journal Information:: Applied and Environmental Microbiology, Vol. 82, Issue 6; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 21 works

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