Fabrication of microstructured binary polymer brush “corrals” with integral pH sensing for studies of proton transport in model membrane systems

Madsen, J.; Ducker, R. E.; Al Jaf, O.; Cartron, M. L.; Alswieleh, A. M.; Smith, C. H.; Hunter, C. N.; Armes, S. P.; Leggett, G. J.

doi:10.1039/C7SC04424K

Title: Fabrication of microstructured binary polymer brush “corrals” with integral pH sensing for studies of proton transport in model membrane systems

Journal Article · Mon Jan 01 00:00:00 EST 2018 · Chemical Science

DOI:https://doi.org/10.1039/C7SC04424K· OSTI ID:1418408

^[1]; Ducker, R. E. ^[1]; Al Jaf, O. ^[1]; Cartron, M. L. ^[2]; Alswieleh, A. M. ^[3]; Smith, C. H. ^[1];

^[2];

^[1];

^[4]

Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
King Saud University, Riyadh, Saudi Arabia
Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK, Krebs Institute for Mechanistic Biology

Binary brush structures consisting of poly(cysteine methacrylate) (PCysMA) “corrals” enclosed within poly(oligoethylene glycol methyl ether methacrylate) (POEGMA) “walls” are fabricated simply and efficiently using a two-step photochemical process. First, the C–Cl bonds of 4-(chloromethyl)phenylsilane monolayers are selectively converted into carboxylic acid groups by patterned exposure to UV light through a mask and POEGMA is grown from unmodified chlorinated regions by surface-initiated atom-transfer radical polymerisation (ATRP). Incorporation of a ratiometric fluorescent pH indicator, Nile Blue 2-(methacryloyloxy)ethyl carbamate (NBC), into the polymer brushes facilitates assessment of local changes in pH using a confocal laser scanning microscope with spectral resolution capability. Moreover, the dye label acts as a radical spin trap, enabling removal of halogen end-groups from the brushes via in situ dye addition during the polymerisation process. Second, an initiator is attached to the carboxylic acid-functionalised regions formed by UV photolysis in the patterning step, enabling growth of PCysMA brushes by ATRP. Transfer of the system to THF, a poor solvent for PCysMA, causes collapse of the PCysMA brushes. At the interface between the collapsed brush and solvent, selective derivatisation of amine groups is achieved by reaction with excess glutaraldehyde, facilitating attachment of aminobutyl(nitrile triacetic acid) (NTA). The PCysMA brush collapse is reversed on transfer to water, leaving it fully expanded but only functionalized at the brush–water interface. Following complexation of NTA with Ni²⁺, attachment of histidine-tagged proteorhodopsin and lipid deposition, light-activated transport of protons into the brush structure is demonstrated by measuring the ratiometric response of NBC in the POEGMA walls.

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Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001035

OSTI ID:: 1418408

Alternate ID(s):: OSTI ID: 1470605

Journal Information:: Chemical Science, Journal Name: Chemical Science Vol. 9 Journal Issue: 8; ISSN 2041-6520

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 24 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Fabrication of microstructured binary polymer brush “corrals” with integral pH sensing for studies of proton transport in model membrane systems

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