Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes

Southall, June; Henry, Sarah L.; Gardiner, Alastair T.; Roszak, Aleksander W.; Mullen, William; Carey, Anne-Marie; Kelly, Sharon M.; de Percin Northumberland, Claire Ortmann; Cogdell, Richard J.

doi:10.1007/s11120-017-0386-7

Title: Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes

Journal Article · Wed May 31 00:00:00 EDT 2017 · Photosynthesis Research

DOI:https://doi.org/10.1007/s11120-017-0386-7· OSTI ID:1470114

Southall, June ^[1]; Henry, Sarah L. ^[1]; Gardiner, Alastair T. ^[1]; Roszak, Aleksander W. ^[1]; Mullen, William ^[1]; Carey, Anne-Marie ^[2]; Kelly, Sharon M. ^[1]; de Percin Northumberland, Claire Ortmann ^[1]; Cogdell, Richard J. ^[1]

Univ. of Glasgow, Scotland (United Kingdom)
Arizona State Univ., Tempe, AZ (United States)

Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBA_d only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.

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Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001035

OSTI ID:: 1470114

Journal Information:: Photosynthesis Research, Vol. 135, Issue 1-3; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; ISSN 0166-8595

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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