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Title: Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Biological Electron Transfer and Catalysis (BETCy)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1469818
DOE Contract Number:  
SC0012518
Resource Type:
Journal Article
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 292; Journal Issue: 38; Related Information: BETCy partners with Montana State University (lead); Arizona State University; National Renewable Energy Laboratory; University of Georgia; University of Kentucky; University of Washington; Utah State University; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
solar (fuels), biofuels (including algae and biomass), bio-inspired, hydrogen and fuel cells

Citation Formats

Pence, Natasha, Tokmina-Lukaszewska, Monika, Yang, Zhi-Yong, Ledbetter, Rhesa N., Seefeldt, Lance C., Bothner, Brian, and Peters, John W. Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle. United States: N. p., 2017. Web. doi:10.1074/jbc.M117.801548.
Pence, Natasha, Tokmina-Lukaszewska, Monika, Yang, Zhi-Yong, Ledbetter, Rhesa N., Seefeldt, Lance C., Bothner, Brian, & Peters, John W. Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle. United States. doi:10.1074/jbc.M117.801548.
Pence, Natasha, Tokmina-Lukaszewska, Monika, Yang, Zhi-Yong, Ledbetter, Rhesa N., Seefeldt, Lance C., Bothner, Brian, and Peters, John W. Mon . "Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle". United States. doi:10.1074/jbc.M117.801548.
@article{osti_1469818,
title = {Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle},
author = {Pence, Natasha and Tokmina-Lukaszewska, Monika and Yang, Zhi-Yong and Ledbetter, Rhesa N. and Seefeldt, Lance C. and Bothner, Brian and Peters, John W.},
abstractNote = {},
doi = {10.1074/jbc.M117.801548},
journal = {Journal of Biological Chemistry},
issn = {0021-9258},
number = 38,
volume = 292,
place = {United States},
year = {2017},
month = {8}
}

Works referenced in this record:

Thermodynamics of nucleotide interactions with the Azotobacter vinelandii nitrogenase iron protein
journal, January 1999

  • Lanzilotta, William N.; Parker, Vernon D.; Seefeldt, Lance C.
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1429, Issue 2
  • DOI: 10.1016/S0167-4838(98)00251-9

The nitrogenase FeMo-cofactor and P-cluster pair: 2.2 A resolution structures
journal, May 1993


Catalytic and Biophysical Properties of a Nitrogenase Apo-MoFe Protein Produced by a n ifB -Deletion Mutant of Azotobacter v inelandii
journal, September 1998

  • Christiansen, Jason; Goodwin, Paul J.; Lanzilotta, William N.
  • Biochemistry, Vol. 37, Issue 36
  • DOI: 10.1021/bi981165b

Insights into Nucleotide Signal Transduction in Nitrogenase:  Structure of an Iron Protein with MgADP Bound ,
journal, December 2000

  • Jang, Se Bok; Seefeldt, Lance C.; Peters, John W.
  • Biochemistry, Vol. 39, Issue 48
  • DOI: 10.1021/bi001705g

Electron transfer in nitrogenase catalysis
journal, April 2012

  • Seefeldt, Lance C.; Hoffman, Brian M.; Dean, Dennis R.
  • Current Opinion in Chemical Biology, Vol. 16, Issue 1-2
  • DOI: 10.1016/j.cbpa.2012.02.012

Nitrogenase MoFe-Protein at 1.16 A Resolution: A Central Ligand in the FeMo-Cofactor
journal, September 2002


Structural models for the metal centers in the nitrogenase molybdenum-iron protein
journal, September 1992


A Conformational Mimic of the MgATP-Bound “On State” of the Nitrogenase Iron Protein ,
journal, February 2004

  • Sen, Sanchayita; Igarashi, Robert; Smith, Archer
  • Biochemistry, Vol. 43, Issue 7
  • DOI: 10.1021/bi0358465

Mechanism of Nitrogen Fixation by Nitrogenase: The Next Stage
journal, January 2014

  • Hoffman, Brian M.; Lukoyanov, Dmitriy; Yang, Zhi-Yong
  • Chemical Reviews, Vol. 114, Issue 8
  • DOI: 10.1021/cr400641x

Nitrogenase and nitrogenase reductase associate and dissociate with each catalytic cycle.
journal, June 1978

  • Hageman, R. V.; Burris, R. H.
  • Proceedings of the National Academy of Sciences, Vol. 75, Issue 6
  • DOI: 10.1073/pnas.75.6.2699

Mechanism of Mo-Dependent Nitrogenase
journal, June 2009


Conformational Gating of Electron Transfer from the Nitrogenase Fe Protein to MoFe Protein
journal, May 2010

  • Danyal, Karamatullah; Mayweather, Diana; Dean, Dennis R.
  • Journal of the American Chemical Society, Vol. 132, Issue 20
  • DOI: 10.1021/ja101737f

Role of magnesium adenosine 5'-triphosphate in the hydrogen evolution reaction catalyzed by nitrogenase from Azotobacter vinelandii
journal, May 1980

  • Hageman, Robert V.; Orme-Johnson, William H.; Burris, R. H.
  • Biochemistry, Vol. 19, Issue 11
  • DOI: 10.1021/bi00552a009

Electron transfer precedes ATP hydrolysis during nitrogenase catalysis
journal, September 2013

  • Duval, S.; Danyal, K.; Shaw, S.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
  • DOI: 10.1073/pnas.1311218110

Nitrogenase of Azotobacter vinelandii : Kinetic Analysis of the Fe Protein Redox Cycle
journal, December 1998

  • Duyvis, Martina G.; Wassink, Hans; Haaker, Huub
  • Biochemistry, Vol. 37, Issue 50
  • DOI: 10.1021/bi981509y

Evidence That the P i Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle
journal, June 2016


Conformational Equilibria and Rates of Localized Motion within Hepatitis B Virus Capsids
journal, January 2008

  • Hilmer, Jonathan K.; Zlotnick, Adam; Bothner, Brian
  • Journal of Molecular Biology, Vol. 375, Issue 2
  • DOI: 10.1016/j.jmb.2007.10.044

Elucidating the Mechanism of Nucleotide-Dependent Changes in the Redox Potential of the [4Fe-4S] Cluster in Nitrogenase Iron Protein:  The Role of Phenylalanine 135
journal, January 1996

  • Ryle, Matthew J.; Lanzilotta, William N.; Seefeldt, Lance C.
  • Biochemistry, Vol. 35, Issue 29
  • DOI: 10.1021/bi9608572

The interpretation of protein structures: Estimation of static accessibility
journal, February 1971


MgATP-Bound and Nucleotide-Free Structures of a Nitrogenase Protein Complex between the Leu 127Δ-Fe-Protein and the MoFe-Protein ,
journal, January 2001

  • Chiu, Hsiu-Ju; Peters, John W.; Lanzilotta, William N.
  • Biochemistry, Vol. 40, Issue 3
  • DOI: 10.1021/bi001645e

Evidence for Interstitial Carbon in Nitrogenase FeMo Cofactor
journal, November 2011


Structure of ADP·AIF4–-stabilized nitrogenase complex and its implications for signal transduction
journal, May 1997

  • Schindelin, Hermann; Kisker, Caroline; Schlessman, Jamie L.
  • Nature, Vol. 387, Issue 6631
  • DOI: 10.1038/387370a0

Mechanism of Molybdenum Nitrogenase
journal, January 1996

  • Burgess, Barbara K.; Lowe, David J.
  • Chemical Reviews, Vol. 96, Issue 7
  • DOI: 10.1021/cr950055x

X-ray Emission Spectroscopy Evidences a Central Carbon in the Nitrogenase Iron-Molybdenum Cofactor
journal, November 2011


Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum
journal, July 1998

  • Schlessman, Jamie L.; Woo, Deborah; Joshua-Tor, Leemor
  • Journal of Molecular Biology, Vol. 280, Issue 4
  • DOI: 10.1006/jmbi.1998.1898

Electron Transfer within Nitrogenase: Evidence for a Deficit-Spending Mechanism
journal, November 2011

  • Danyal, Karamatullah; Dean, Dennis R.; Hoffman, Brian M.
  • Biochemistry, Vol. 50, Issue 43
  • DOI: 10.1021/bi201003a

Evidence of Viral Capsid Dynamics Using Limited Proteolysis and Mass Spectrometry
journal, January 1998

  • Bothner, Brian; Dong, X. Fan; Bibbs, Lisa
  • Journal of Biological Chemistry, Vol. 273, Issue 2
  • DOI: 10.1074/jbc.273.2.673

Characterization of a Variant Iron Protein of Nitrogenase That Is Impaired in Its Ability to Adopt the MgATP-induced Conformational Change
journal, July 1998

  • Bursey, Evan H.; Burgess, Barbara K.
  • Journal of Biological Chemistry, Vol. 273, Issue 27
  • DOI: 10.1074/jbc.273.27.16927

Nitrogenase Complexes: Multiple Docking Sites for a Nucleotide Switch Protein
journal, August 2005


The Coupling of Electron Transfer in Nitrogenase to the Hydrolysis of Magnesium Adenosine Triphosphate
journal, August 1979

  • Thorneley, Roger N. F.; Lowe, David J.; Eday, Robert R.
  • Biochemical Society Transactions, Vol. 7, Issue 4
  • DOI: 10.1042/bst0070633

Evidence for a Central Role of Lysine 15 of Azotobacter vinelandii Nitrogenase Iron Protein in Nucleotide Binding and Protein Conformational Changes
journal, June 1995

  • Ryle, Matthew J.; Lanzilotta, William N.; Mortenson, Leonard E.
  • Journal of Biological Chemistry, Vol. 270, Issue 22
  • DOI: 10.1074/jbc.270.22.13112