Coordination of the Ser2056 and Thr2609 Clusters of DNA-PKcs in Regulating Gamma Rays and Extremely Low Fluencies of Alpha-Particle Irradiation to G₀/G₁ Phase Cells

Journal Article · Tue Jan 24 00:00:00 EST 2017 · Radiation Research

DOI:https://doi.org/10.1667/RR14679.1· OSTI ID:1469798

Nagasawa, Hatsumi ^[1]; Lin, Yu-Fen ^[2]; Kato, Takamitsu A. ^[1]; Brogan, John R. ^[1]; Shih, Hung-Ying ^[2]; Kurimasa, Akihiro ^[3]; Bedford, Joel S. ^[1]; Chen, Benjamin P. C. ^[2]; Little, John B. ^[4]

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1. Colorado State Univ., Fort Collins, CO (United States). Dept. of Environmental and Radiological Health Sciences
2. Univ. of Texas Southwestern Medical Center at Dallas, Dallas, TX (United States). Dept. of Radiation Oncology
3. Tohoku Medical and Pharmaceutical Univ., Sendai (Japan)
4. Harvard School of Public Health, Boston, MA (United States). Dept. of Genetics and Complex Diseases

The catalytic subunit of DNA dependent protein kinase (DNA-PKcs) and its kinase activity are critical for mediation of non-homologous end-joining (NHEJ) of DNA double-strand breaks (DSB) in mammalian cells after gamma-ray irradiation. Additionally, DNA-PKcs phosphorylations at the T2609 cluster and the S2056 cluster also affect DSB repair and cellular sensitivity to gamma radiation. Previously we reported that phosphorylations within these two regions affect not only NHEJ but also homologous recombination repair (HRR) dependent DSB repair. In this study, we further examine phenotypic effects on cells bearing various combinations of mutations within either or both regions. Effects studied included cell killing as well as chromosomal aberration induction after 0.5–8 Gy gamma-ray irradiation delivered to synchronized cells during the G0/G1 phase of the cell cycle. Blocking phosphorylation within the T2609 cluster was most critical regarding sensitization and depended on the number of available phosphorylation sites. It was also especially interesting that only one substitution of alanine in each of the two clusters separately abolished the restoration of wild-type sensitivity by DNA-PKcs. Similar patterns were seen for induction of chromosomal aberrations, reflecting their connection to cell killing. To study possible change in coordination between HRR and NHEJ directed repair in these DNA-PKcs mutant cell lines, we compared the induction of sister chromatid exchanges (SCEs) by very low fluencies of alpha particles with mutant cells defective in the HRR pathway that is required for induction of SCEs. Finally, levels of true SCEs induced by very low fluence of alpha-particle irradiation normally seen in wild-type cells were only slightly decreased in the S2056 cluster mutants, but were completely abolished in the T2609 cluster mutants and were indistinguishable from levels seen in HRR deficient cells. Again, a single substitution in the S2056 together with a single substitution in the T2609 cluster abolished SCE formation and thus also effectively interferes with HRR.

• Research Organization: Colorado State Univ., Fort Collins, CO (United States)
• Sponsoring Organization: USDOE; National Institutes of Health (NIH)
• Grant/Contract Number: FG02-07ER64350; FG02-05ER64089
• OSTI ID: 1469798
• Journal Information: Radiation Research, Vol. 187, Issue 2; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: United States
• Language: English

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