Functional Analysis of Cellulose Synthase CesA4 and CesA6 Genes in Switchgrass (Panicum virgatum) by Overexpression and RNAi-Mediated Gene Silencing

Mazarei, Mitra; Baxter, Holly L.; Li, Mi; Biswal, Ajaya K.; Kim, Keonhee; Meng, Xianzhi; Pu, Yunqiao; Wuddineh, Wegi A.; Zhang, Ji-Yi; Turner, Geoffrey B.; Sykes, Robert W.; Davis, Mark F.; Udvardi, Michael K.; Wang, Zeng-Yu; Mohnen, Debra; Ragauskas, Arthur J.; Labbé, Nicole; Stewart, C. Neal

doi:10.3389/fpls.2018.01114

Title: Functional Analysis of Cellulose Synthase CesA4 and CesA6 Genes in Switchgrass (Panicum virgatum) by Overexpression and RNAi-Mediated Gene Silencing

Journal Article · Fri Aug 03 00:00:00 EDT 2018 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2018.01114· OSTI ID:1468520

Mazarei, Mitra ^[1]; Baxter, Holly L. ^[1];

^[2]; Biswal, Ajaya K. ^[3]; Kim, Keonhee ^[4]; Meng, Xianzhi ^[5];

^[2]; Wuddineh, Wegi A. ^[1]; Zhang, Ji-Yi ^[6]; Turner, Geoffrey B. ^[7]; Sykes, Robert W. ^[7];

^[7]; Udvardi, Michael K. ^[6]; Wang, Zeng-Yu ^[6]; Mohnen, Debra ^[3]; Ragauskas, Arthur J. ^[8]; Labbé, Nicole ^[4]; Stewart, C. Neal ^[1]

Univ. of Tennessee, Knoxville, TN (United States). Department of Plant Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center and Biosciences Division, Joint Institute for Biological Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical and Biomolecular Engineering
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Noble Research Institute, Ardmore, OK (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center and Biosciences Division, Joint Institute for Biological Science; Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical and Biomolecular Engineering

Switchgrass (Panicum virgatum L.) is a leading lignocellulosic bioenergy feedstock. Cellulose is a major component of the plant cell walls and the primary substrate for saccharification. Accessibility of cellulose to enzymatic breakdown into fermentable sugars is limited by the presence of lignin in the plant cell wall. In this study, putatively novel switchgrass secondary cell wall cellulose synthase PvCesA4 and primary cell wall PvCesA6 genes were identified and their functional role in cellulose synthesis and cell wall composition was examined by overexpression and knockdown of the individual genes in switchgrass. The endogenous expression of PvCesA4 and PvCesA6 genes varied among including roots, leaves, stem, and reproductive tissues. Increasing or decreasing PvCesA4 and PvCesA6 expression to extreme levels in the transgenic lines resulted in decreased biomass production. PvCesA6-overexpressing lines had reduced lignin content and syringyl/guaiacyl lignin monomer ratio accompanied by increased sugar release efficiency, suggesting an impact of PvCesA6 expression levels on lignin biosynthesis. Cellulose content and cellulose crystallinity were decreased, while xylan content was increased in PvCesA4 and PvCesA6 overexpression or knockdown lines. The increase in xylan content suggests that the amount of non-cellulosic cell wall polysaccharide was modified in these plants. Taken together, the results show that the manipulation of the cellulose synthase genes alters the cell wall composition and availability of cellulose as a bioprocessing substrate.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308; AC05-00OR22725

OSTI ID:: 1468520

Alternate ID(s):: OSTI ID: 1468056

Report Number(s):: NREL/JA-2700-72304

Journal Information:: Frontiers in Plant Science, Vol. 9; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 19 works

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Title: Functional Analysis of Cellulose Synthase CesA4 and CesA6 Genes in Switchgrass (Panicum virgatum) by Overexpression and RNAi-Mediated Gene Silencing

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