Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer

Liu, Na; Ni, Shuzhen; Ragauskas, Arthur J.; Meng, Xianzhi; Hao, Naijia; Fu, Yingjuan

doi:10.1016/j.jbiotec.2018.01.015

Title: Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer

Journal Article · Sun Feb 04 00:00:00 EST 2018 · Journal of Biotechnology

DOI:https://doi.org/10.1016/j.jbiotec.2018.01.015· OSTI ID:1468142

Liu, Na ^[1]; Ni, Shuzhen ^[2]; Ragauskas, Arthur J. ^[3]; Meng, Xianzhi ^[3]; Hao, Naijia ^[3]; Fu, Yingjuan ^[2]

Qilu University of Technology (Shandong Academy of Sciences), Jinan (China); Univ. of Tennessee, Knoxville, TN (United States)
Qilu University of Technology (Shandong Academy of Sciences), Jinan (China)
Univ. of Tennessee, Knoxville, TN (United States)

In this paper, an effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions was developed using laccase as the catalyst. The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength. Solid-state ¹³C NMR spectrum confirmed the incorporation of HP onto chitosan. X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan. The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio. A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM). The laccase/HP treatment of chitosan improved the thermal stability of copolymer. More significantly, the HP functionalized chitosan showed greatly improved ABTS⁺ and DPPH radicals scavenging capacity, compared with pure chitosan. The hydrophobicity property of the HP functionalized chitosan also significantly increased although its tensile strength decreased. Finally, this new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.

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Research Organization:: Univ. of Tennessee, Knoxville, TN (United States); Qilu Univ. of Technology, Jinan (China)

Sponsoring Organization:: USDOE; Key Research and Development Program of Shandong Province (China); National Natural Science Fund (China); National Natural Science Foundation of China (NSFC)

Grant/Contract Number:: AC05-00OR22725; 2016GGX108002; 31370581; 31540009

OSTI ID:: 1468142

Journal Information:: Journal of Biotechnology, Vol. 269; ISSN 0168-1656

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 14 works

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
chitosan
laccase
4-hexyloxyphenol
antioxidant
hydrophobic