Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase
Abstract
The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substituent effects can modulate enzyme activity in DHP, we investigated its reactiviy against a panel of o-guaiacol substrates given their presence (from native/halogenated and non-native/anthropogenic sources) in the benthic environment that A. ornata inhabits. Using biochemical assays supported by spectroscopic, spectrometric, and structural studies, DHP was found to catalyze the H2O2-dependent oxidative dehalogenation of 4-haloguaiacols (F, Cl, and Br) to 2-methoxybenzoquinone (2-MeOBQ). 18O labeling studies confirmed that O atom incorporation was derived exclusively from water, consistent with substrate oxidation via a peroxidase-based mechanism. Here, the 2-MeOBQ product further reduced DHP to its oxyferrous state, providing a link between the substrate oxidation and O2 carrier functions of DHP. Nonnative substrates resulted in polymerization of the initial substrate with varying degrees of oxidation, with 2-MeOBQ identified as a minor product. When viewed alongside the reactivity of previously studied phenolic substrates, the results presented here show that simple substituent effects can serve as functional switches between peroxidase and peroxygenase activities in this multifunctional catalytic globin. Moremore »
- Authors:
-
- North Carolina State Univ., Raleigh, NC (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
- OSTI Identifier:
- 1467729
- Grant/Contract Number:
- W-31-109-Eng-38; CHE-1150709; CHE-1609446; CHE-1659690
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Biochemistry
- Additional Journal Information:
- Journal Volume: 57; Journal Issue: 30; Journal ID: ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; redox reactions; bioinorganic chemistry; peptides and proteins; surface interactions; oxidation; globin; guaiacol; heme; peroxidase; peroxygenase; structure-function
Citation Formats
McGuire, Ashlyn H., Carey, Leiah M., de Serrano, Vesna, Dali, Safaa, and Ghiladi, Reza A. Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase. United States: N. p., 2018.
Web. doi:10.1021/acs.biochem.8b00540.
McGuire, Ashlyn H., Carey, Leiah M., de Serrano, Vesna, Dali, Safaa, & Ghiladi, Reza A. Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase. United States. https://doi.org/10.1021/acs.biochem.8b00540
McGuire, Ashlyn H., Carey, Leiah M., de Serrano, Vesna, Dali, Safaa, and Ghiladi, Reza A. 2018.
"Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase". United States. https://doi.org/10.1021/acs.biochem.8b00540. https://www.osti.gov/servlets/purl/1467729.
@article{osti_1467729,
title = {Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase},
author = {McGuire, Ashlyn H. and Carey, Leiah M. and de Serrano, Vesna and Dali, Safaa and Ghiladi, Reza A.},
abstractNote = {The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substituent effects can modulate enzyme activity in DHP, we investigated its reactiviy against a panel of o-guaiacol substrates given their presence (from native/halogenated and non-native/anthropogenic sources) in the benthic environment that A. ornata inhabits. Using biochemical assays supported by spectroscopic, spectrometric, and structural studies, DHP was found to catalyze the H2O2-dependent oxidative dehalogenation of 4-haloguaiacols (F, Cl, and Br) to 2-methoxybenzoquinone (2-MeOBQ). 18O labeling studies confirmed that O atom incorporation was derived exclusively from water, consistent with substrate oxidation via a peroxidase-based mechanism. Here, the 2-MeOBQ product further reduced DHP to its oxyferrous state, providing a link between the substrate oxidation and O2 carrier functions of DHP. Nonnative substrates resulted in polymerization of the initial substrate with varying degrees of oxidation, with 2-MeOBQ identified as a minor product. When viewed alongside the reactivity of previously studied phenolic substrates, the results presented here show that simple substituent effects can serve as functional switches between peroxidase and peroxygenase activities in this multifunctional catalytic globin. More broadly, when recent findings on DHP activity with nitrophenols and azoles are included, the results presented here further demonstrate the breadth of heterocyclic compounds of anthropogenic origin that can potentially disrupt marine hemoglobins or function as environmental stressors, findings that may be important when assessing the environmental impact of these pollutants (and their metabolites) on aquatic systems.},
doi = {10.1021/acs.biochem.8b00540},
url = {https://www.osti.gov/biblio/1467729},
journal = {Biochemistry},
issn = {0006-2960},
number = 30,
volume = 57,
place = {United States},
year = {Wed Jun 27 00:00:00 EDT 2018},
month = {Wed Jun 27 00:00:00 EDT 2018}
}
Web of Science
Works referenced in this record:
Treatment of pulp and paper mill wastewater—a review
journal, October 2004
- Pokhrel, D.; Viraraghavan, T.
- Science of The Total Environment, Vol. 333, Issue 1-3
Degradation of 4-Chloroguaiacol by Dark Fenton and Solar Photo-Fenton Advanced Oxidation Processes
journal, December 2009
- Samet, Youssef; Ayadi, Mohamed; Abdelhedi, Ridha
- Water Environment Research, Vol. 81, Issue 12
Kinetic degradation of the pollutant guaiacol by dark Fenton and solar photo-Fenton processes
journal, May 2011
- Samet, Youssef; Wali, Ines; Abdelhédi, Ridha
- Environmental Science and Pollution Research, Vol. 18, Issue 9
Unraveling Pathways of Guaiacol Nitration in Atmospheric Waters: Nitrite, A Source of Reactive Nitronium Ion in the Atmosphere
journal, July 2015
- Kroflič, Ana; Grilc, Miha; Grgić, Irena
- Environmental Science & Technology, Vol. 49, Issue 15
Biosynthesis of polybrominated aromatic organic compounds by marine bacteria
journal, June 2014
- Agarwal, Vinayak; El Gamal, Abrahim A.; Yamanaka, Kazuya
- Nature Chemical Biology, Vol. 10, Issue 8
Solubility and Infinite Dilution Activity Coefficient for 5-Chlorovanillin and 4-Chloroguaiacol in Water over the Temperature Range 280 to 363 K
journal, March 2000
- Larachi, Faïçal; Leroux, Marc; Hamoudi, Safia
- Journal of Chemical & Engineering Data, Vol. 45, Issue 2
Does toxicity of aromatic pollutants increase under remote atmospheric conditions?
journal, March 2015
- Kroflič, Ana; Grilc, Miha; Grgić, Irena
- Scientific Reports, Vol. 5, Issue 1
Atmospheric reactivity of hydroxyl radicals with guaiacol (2-methoxyphenol), a biomass burning emitted compound: Secondary organic aerosol formation and gas-phase oxidation products
journal, April 2014
- Lauraguais, Amélie; Coeur-Tourneur, Cécile; Cassez, Andy
- Atmospheric Environment, Vol. 86
Acute toxicity of emerging atmospheric pollutants from wood lignin due to biomass burning
journal, September 2017
- Pflieger, Maryline; Kroflič, Ana
- Journal of Hazardous Materials, Vol. 338
Oxidation of Guaiacol by Lignin Peroxidase: ROLE OF VERATRYL ALCOHOL
journal, September 1995
- Koduri, Rao S.; Tien, Ming
- Journal of Biological Chemistry, Vol. 270, Issue 38
Engineered Bacillus pumilus laccase-like multi-copper oxidase for enhanced oxidation of the lignin model compound guaiacol
journal, May 2017
- Ihssen, Julian; Jankowska, Dagmara; Ramsauer, Thomas
- Protein Engineering, Design and Selection, Vol. 30, Issue 6
Single-walled carbon nanotube release affects the microbial enzyme-catalyzed oxidation processes of organic pollutants and lignin model compounds in nature
journal, November 2016
- Chen, Ming; Qin, Xiaosheng; Zeng, Guangming
- Chemosphere, Vol. 163
Structural and spectroscopic characterisation of a heme peroxidase from sorghum
journal, December 2015
- Nnamchi, Chukwudi I.; Parkin, Gary; Efimov, Igor
- JBIC Journal of Biological Inorganic Chemistry, Vol. 21, Issue 1
Identifying the Elusive Sites of Tyrosyl Radicals in Cytochrome c Peroxidase: Implications for Oxidation of Substrates Bound at a Site Remote from the Heme
journal, June 2014
- Miner, Kyle D.; Pfister, Thomas D.; Hosseinzadeh, Parisa
- Biochemistry, Vol. 53, Issue 23
Influence of temperature, pH and metal ions on guaiacol oxidation of purified laccase from Leptographium qinlingensis
journal, November 2013
- Hu, Xia; Wang, Chunyan; Wang, Le
- World Journal of Microbiology and Biotechnology, Vol. 30, Issue 4
Two Oxidation Sites for Low Redox Potential Substrates: A DIRECTED MUTAGENESIS, KINETIC, AND CRYSTALLOGRAPHIC STUDY ON PLEUROTUS ERYNGII VERSATILE PEROXIDASE
journal, October 2012
- Morales, María; Mate, María J.; Romero, Antonio
- Journal of Biological Chemistry, Vol. 287, Issue 49
An enzymatic globin from a marine worm
journal, September 1999
- Lebioda, Lukasz; LaCount, Michael W.; Zhang, Erli
- Nature, Vol. 401, Issue 6752
The dehaloperoxidase paradox
journal, April 2012
- Franzen, Stefan; Thompson, Matthew K.; Ghiladi, Reza A.
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1824, Issue 4
Hemoglobins of two terebellid polychaetes: Enoplobranchus sanguineus and Amphitrite ornata
journal, January 1977
- Weber, Roy E.; Mangum, Charlotte; Steinman, Howard
- Comparative Biochemistry and Physiology Part A: Physiology, Vol. 56, Issue 2
The Crystal Structure and Amino Acid Sequence of Dehaloperoxidase from Amphitrite ornata Indicate Common Ancestry with Globins
journal, June 2000
- LaCount, Michael W.; Zhang, Erli; Chen, Yung Pin
- Journal of Biological Chemistry, Vol. 275, Issue 25
Peroxygenase and Oxidase Activities of Dehaloperoxidase-Hemoglobin from Amphitrite ornata
journal, May 2014
- Barrios, David A.; D’Antonio, Jennifer; McCombs, Nikolette L.
- Journal of the American Chemical Society, Vol. 136, Issue 22
Oxidation of pyrrole by dehaloperoxidase-hemoglobin: chemoenzymatic synthesis of pyrrolin-2-ones
journal, January 2017
- McCombs, Nikolette L.; Smirnova, Tatyana; Ghiladi, Reza A.
- Catalysis Science & Technology, Vol. 7, Issue 14
Influence of heme environment structure on dioxygen affinity for the dual function Amphitrite ornata hemoglobin/dehaloperoxidase. Insights into the evolutional structure–function adaptations
journal, March 2014
- Sun, Shengfang; Sono, Masanori; Wang, Chunxue
- Archives of Biochemistry and Biophysics, Vol. 545
Complexes of Dual-Function Hemoglobin/Dehaloperoxidase with Substrate 2,4,6-Trichlorophenol Are Inhibitory and Indicate Binding of Halophenol to Compound I
journal, August 2013
- Wang, Chunxue; Lovelace, Leslie L.; Sun, Shengfang
- Biochemistry, Vol. 52, Issue 36
Amphitrite ornata Dehaloperoxidase (DHP): Investigations of Structural Factors That Influence the Mechanism of Halophenol Dehalogenation Using “Peroxidase-like” Myoglobin Mutants and “Myoglobin-like” DHP Mutants
journal, September 2011
- Du, Jing; Huang, Xiao; Sun, Shengfang
- Biochemistry, Vol. 50, Issue 38
How nature tunes isoenzyme activity in the multifunctional catalytic globin dehaloperoxidase from Amphitrite ornata
journal, February 2018
- Carey, Leiah M.; Gavenko, Roman; Svistunenko, Dimitri A.
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1866, Issue 2
Selective tuning of activity in a multifunctional enzyme as revealed in the F21W mutant of dehaloperoxidase B from Amphitrite ornata
journal, November 2017
- Carey, Leiah M.; Kim, Kyung Beom; McCombs, Nikolette L.
- JBIC Journal of Biological Inorganic Chemistry, Vol. 23, Issue 2
Tyrosyl Radicals in Dehaloperoxidase
journal, November 2013
- Dumarieh, Rania; D'Antonio, Jennifer; Deliz-Liang, Alexandria
- Journal of Biological Chemistry, Vol. 288, Issue 46
Compound ES of Dehaloperoxidase Decays via Two Alternative Pathways Depending on the Conformation of the Distal Histidine
journal, December 2010
- Thompson, Matthew K.; Franzen, Stefan; Ghiladi, Reza A.
- Journal of the American Chemical Society, Vol. 132, Issue 49
Characterization of Dehaloperoxidase Compound ES and Its Reactivity with Trihalophenols †
journal, February 2009
- Feducia, Jeremiah; Dumarieh, Rania; Gilvey, Lauren B. G.
- Biochemistry, Vol. 48, Issue 5
Nonmicrobial Nitrophenol Degradation via Peroxygenase Activity of Dehaloperoxidase-Hemoglobin from Amphitrite ornata
journal, April 2016
- McCombs, Nikolette L.; D’Antonio, Jennifer; Barrios, David A.
- Biochemistry, Vol. 55, Issue 17
Interaction of Azole-Based Environmental Pollutants with the Coelomic Hemoglobin from Amphitrite ornata : A Molecular Basis for Toxicity
journal, April 2017
- McCombs, Nikolette L.; Moreno-Chicano, Tadeo; Carey, Leiah M.
- Biochemistry, Vol. 56, Issue 17
Amphitrite ornata, a Marine Worm, Contains Two Dehaloperoxidase Genes
journal, May 2001
- Han, Kaiping; Woodin, Sarah A.; Lincoln, David E.
- Marine Biotechnology, Vol. 3, Issue 3
Structure of dehaloperoxidase B at 1.58 Å resolution and structural characterization of the AB dimer from Amphitrite ornata
journal, April 2010
- de Serrano, Vesna; D'Antonio, Jennifer; Franzen, Stefan
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 5
Probing the Oxyferrous and Catalytically Active Ferryl States of Amphitrite ornata Dehaloperoxidase by Cryoreduction and EPR/ENDOR Spectroscopy. Detection of Compound I
journal, October 2010
- Davydov, Roman; Osborne, Robert L.; Shanmugam, Muralidharan
- Journal of the American Chemical Society, Vol. 132, Issue 42
Spectroscopic and Mechanistic Investigations of Dehaloperoxidase B from Amphitrite ornata
journal, August 2010
- D’Antonio, Jennifer; D’Antonio, Edward L.; Thompson, Matthew K.
- Biochemistry, Vol. 49, Issue 31
Reactivity of Deoxy- and Oxyferrous Dehaloperoxidase B from Amphitrite ornata: Identification of Compound II and Its Ferrous–Hydroperoxide Precursor
journal, July 2011
- D’Antonio, Jennifer; Ghiladi, Reza A.
- Biochemistry, Vol. 50, Issue 27
Kinetic Study of the Inhibition Mechanism of Dehaloperoxidase-Hemoglobin A by 4-Bromophenol
journal, July 2013
- Zhao, Jing; Franzen, Stefan
- The Journal of Physical Chemistry B, Vol. 117, Issue 28
The natural production of organobromine compounds
journal, March 2000
- Gribble, Gordon W.
- Environmental Science and Pollution Research, Vol. 7, Issue 1
Role of tryptophan-208 residue in cytochrome c oxidation by ascorbate peroxidase from Leishmania major-kinetic studies on Trp208Phe mutant and wild type enzyme
journal, May 2008
- Yadav, Rajesh K.; Dolai, Subhankar; Pal, Swati
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 5
Immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks
journal, January 2007
- Castro-Forero, Angelines; Jiménez, David; López-Garriga, Juan
- Journal of Applied Polymer Science, Vol. 107, Issue 2
Reactivity of the horseradish peroxidase compounds I and II toward organometallic substrates. A stopped-flow kinetic study of oxidation of ferrocenes
journal, June 1998
- Goral, Vasily; Ryabov, Alexander
- IUBMB Life, Vol. 45, Issue 1
Measuring Binding Affinity of Protein−Ligand Interaction Using Spectrophotometry: Binding of Neutral Red to Riboflavin-Binding Protein
journal, June 2010
- Chenprakhon, Pirom; Sucharitakul, Jeerus; Panijpan, Bhinyo
- Journal of Chemical Education, Vol. 87, Issue 8
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
The CCP4 suite programs for protein crystallography
journal, September 1994
- ,
- Acta Crystallographica Section D Biological Crystallography, Vol. 50, Issue 5, p. 760-763
Features and development of Coot
journal, March 2010
- Emsley, P.; Lohkamp, B.; Scott, W. G.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997
- Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
Towards automated crystallographic structure refinement with phenix.refine
journal, March 2012
- Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel
- Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4
An Unusual Dehalogenating Peroxidase from the Marine Terebellid Polychaete Amphitrite ornata
journal, March 1996
- Chen, Yung Pin; Woodin, Sarah A.; Lincoln, David E.
- Journal of Biological Chemistry, Vol. 271, Issue 9
Internal Binding of Halogenated Phenols in Dehaloperoxidase-Hemoglobin Inhibits Peroxidase Function
journal, September 2010
- Thompson, Matthew K.; Davis, Michael F.; de Serrano, Vesna
- Biophysical Journal, Vol. 99, Issue 5
Guaiacol Oxidation Products in the Enzyme-Activity Assay Reaction by Horseradish Peroxidase Catalysis
journal, July 2004
- Tonami, Hiroyuki; Uyama, Hiroshi; Nagahata, Ritsuko
- Chemistry Letters, Vol. 33, Issue 7
Identification of the Colored Guaiacol Oxidation Product Produced by Peroxidases
journal, July 1997
- Doerge, Daniel R.; Divi, Rao L.; Churchwell, Mona I.
- Analytical Biochemistry, Vol. 250, Issue 1
Oxidative Coupling and Polymerization of Guaiacol, a Lignin Derivative
journal, September 1988
- Simmons, Kathleen E.; Minard, Robert D.; Bollag, Jean-Marc
- Soil Science Society of America Journal, Vol. 52, Issue 5
Structural evidence for stabilization of inhibitor binding by a protein cavity in the dehaloperoxidase-hemoglobin from Amphitrite ornata
journal, May 2011
- de Serrano, Vesna; Franzen, Stefan
- Biopolymers, Vol. 98, Issue 1
Evidence of the Direct Involvement of the Substrate TCP Radical in Functional Switching from Oxyferrous O 2 Carrier to Ferric Peroxidase in the Dual-Function Hemoglobin/Dehaloperoxidase from Amphitrite ornata
journal, July 2014
- Sun, Shengfang; Sono, Masanori; Du, Jing
- Biochemistry, Vol. 53, Issue 30
Functional Switching of Amphitrite ornata Dehaloperoxidase from O 2 -Binding Globin to Peroxidase Enzyme Facilitated by Halophenol Substrate and H 2 O 2
journal, July 2010
- Du, Jing; Sono, Masanori; Dawson, John H.
- Biochemistry, Vol. 49, Issue 29
An unexpected side reaction in the guaiacol assay for peroxidase
journal, September 1992
- Taurog, Alvin; Dorris, Martha L.; Guziec, Frank S.
- Analytical Biochemistry, Vol. 205, Issue 2
Precise Design of Artificial Cofactors for Enhancing Peroxidase Activity of Myoglobin: Myoglobin Mutant H64D Reconstituted with a “Single-Winged Cofactor” Is Equivalent to Native Horseradish Peroxidase in Oxidation Activity
journal, June 2011
- Matsuo, Takashi; Fukumoto, Kazuki; Watanabe, Takuro
- Chemistry - An Asian Journal, Vol. 6, Issue 9
Hybridization of Modified-Heme Reconstitution and Distal Histidine Mutation to Functionalize Sperm Whale Myoglobin
journal, January 2004
- Sato, Hideaki; Hayashi, Takashi; Ando, Tsutomu
- Journal of the American Chemical Society, Vol. 126, Issue 2
Peroxidase Activity of Myoglobin Is Enhanced by Chemical Mutation of Heme-Propionates
journal, September 1999
- Hayashi, Takashi; Hitomi, Yutaka; Ando, Tsutomu
- Journal of the American Chemical Society, Vol. 121, Issue 34
Lignin and Mn Peroxidase-Catalyzed Oxidation of Phenolic Lignin Oligomers †
journal, March 1999
- Banci, Lucia; Ciofi-Baffoni, Simone; Tien, Ming
- Biochemistry, Vol. 38, Issue 10
Dehydrogenation of Phenols. II. Dehydrogenation Polymers from Guaiacol.
journal, January 1960
- Lindgren, Bengt O.; Norman, Nico; Åselius, J.
- Acta Chemica Scandinavica, Vol. 14
The reaction of guaiacol with iron III and chromium VI compounds as a model for wood surface modification
journal, June 1995
- Schmalzl, K. J.; Forsyth, C. M.; Evans, P. D.
- Wood Science and Technology, Vol. 29, Issue 4
Dehydrogenation of Phenols. I. Dehydrogenation of 2,6-Dimethylphenol with Silver Oxide.
journal, January 1960
- Lindgren, Bengt O.; Norman, Nico; Åselius, J.
- Acta Chemica Scandinavica, Vol. 14
Applications of Absorption Spectroelectrochemistry in Artificial Blood Research
journal, December 2004
- McKinley, A. Brent; Kenny, Clare F.; Martin, Melinda S.
- Spectroscopy Letters, Vol. 37, Issue 3
Optically transparent thin layer electrode techniquesfor the study of biological redox systems
journal, January 1979
- Heineman, William R.; Meckstroth, Marilyn L.; Norris, Barbara J.
- Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, Vol. 104
Redox reactivity of the heme Fe3+/Fe2+ couple in native myoglobins and mutants with peroxidase-like activity
journal, June 2007
- Battistuzzi, Gianantonio; Bellei, Marzia; Casella, Luigi
- JBIC Journal of Biological Inorganic Chemistry, Vol. 12, Issue 7
Redox Thermodynamics of the Fe(III)/Fe(II) Couple of Human Myeloperoxidase in Its High-Spin and Low-Spin Forms †
journal, October 2006
- Battistuzzi, Gianantonio; Bellei, Marzia; Zederbauer, Martina
- Biochemistry, Vol. 45, Issue 42
The Asp-His-iron triad of cytochrome c peroxidase controls the reduction potential electronic structure, and coupling of the tryptophan free radical to the heme
journal, April 1993
- Goodin, David B.; McRee, Duncan E.
- Biochemistry, Vol. 32, Issue 13
Hydrogen Bond Network in the Distal Site of Peroxidases: Spectroscopic Properties of Asn70 → Asp Horseradish Peroxidase Mutant †
journal, August 1997
- Tanaka, Motomasa; Nagano, Shingo; Ishimori, Koichiro
- Biochemistry, Vol. 36, Issue 32
The Mechanism of Oxidative Halophenol Dehalogenation by Amphitrite ornata Dehaloperoxidase Is Initiated by H 2 O 2 Binding and Involves Two Consecutive One-Electron Steps: Role of Ferryl Intermediates
journal, April 2009
- Osborne, Robert L.; Coggins, Michael K.; Raner, Gregory M.
- Biochemistry, Vol. 48, Issue 20
Structural and Kinetic Study of an Internal Substrate Binding Site in Dehaloperoxidase-Hemoglobin A from Amphitrite ornata
journal, March 2013
- Zhao, Jing; de Serrano, Vesna; Zhao, Junjie
- Biochemistry, Vol. 52, Issue 14
Nonphotochemical Base-Catalyzed Hydroxylation of 2,6-Dichloroquinone by H 2 O 2 Occurs by a Radical Mechanism
journal, January 2012
- Franzen, Stefan; Sasan, Koroush; Sturgeon, Bradley E.
- The Journal of Physical Chemistry B, Vol. 116, Issue 5
On the function and mechanism of action of peroxidases
journal, August 1976
- Dunford, H.
- Coordination Chemistry Reviews, Vol. 19, Issue 3
Works referencing / citing this record:
High-throughput structures of protein–ligand complexes at room temperature using serial femtosecond crystallography
journal, October 2019
- Moreno-Chicano, Tadeo; Ebrahim, Ali; Axford, Danny
- IUCrJ, Vol. 6, Issue 6