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Title: Tomosaic : efficient acquisition and reconstruction of teravoxel tomography data using limited-size synchrotron X-ray beams

Journal Article · · Journal of Synchrotron Radiation (Online)

X-rays offer high penetration with the potential for tomography of centimetre-sized specimens, but synchrotron beamlines often provide illumination that is only millimetres wide. Here an approach is demonstrated termedTomosaicfor tomographic imaging of large samples that extend beyond the illumination field of view of an X-ray imaging system. This includes software modules for image stitching and calibration, while making use of existing modules available in other packages for alignment and reconstruction. The approach is compatible with conventional beamline hardware, while providing a dose-efficient method of data acquisition. By using parallelization on a distributed computing system, it provides a solution for handling teravoxel-sized or larger datasets that cannot be processed on a single workstation in a reasonable time. Furthermore, using experimental data, the package is shown to provide good quality three-dimensional reconstruction for centimetre-sized samples with sub-micrometre pixel size.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE; National Institutes of Health (NIH)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1465616
Alternate ID(s):
OSTI ID: 1467649
Journal Information:
Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Vol. 25 Journal Issue: 5; ISSN 1600-5775
Publisher:
International Union of Crystallography (IUCr)Copyright Statement
Country of Publication:
Denmark
Language:
English
Citation Metrics:
Cited by: 28 works
Citation information provided by
Web of Science

References (34)

Status and evolution of the ESRF beamline ID19
  • Weitkamp, Timm; Tafforeau, Paul; Boller, Elodie
  • X-RAY OPTICS AND MICROANALYSIS: Proceedings of the 20th International Congress, AIP Conference Proceedings https://doi.org/10.1063/1.3399253
conference January 2010
TomoPy: a framework for the analysis of synchrotron tomographic data journal August 2014
Notizen: Photochemistry of Selenol Esters journal December 1976
A robust procedure for determination of center of rotation in tomography conference October 2004
Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object journal April 2002
Three-Dimensional X-ray Microtomography journal September 1987
Region-of-interest tomography using filtered backprojection: assessing the practical limits: RoI TOMOGRAPHY: ASSESSING THE LIMITS journal December 2010
Recent advances in X-ray microtomography applied to materials journal May 2008
X-ray mosaic nanotomography of large microorganisms journal February 2012
Imaging samples larger than the field of view: the SLS experience journal June 2017
Efficient subpixel image registration algorithms journal January 2008
TXM-Wizard : a program for advanced data collection and evaluation in full-field transmission X-ray microscopy journal January 2012
MPI for Python journal September 2005
Efficient and Secure Transfer, Synchronization, and Sharing of Big Data journal September 2014
Radiography registration for mosaic tomography journal April 2017
Optimization of tomographic reconstruction workflows on geographically distributed resources journal June 2016
TomoBank: a tomographic data repository for computational x-ray science journal February 2018
Globally optimal stitching of tiled 3D microscopic image acquisitions journal April 2009
Scientific data exchange: a schema for HDF5-based storage of raw and analyzed data journal October 2014
MPI for Python: Performance improvements and MPI-2 extensions journal May 2008
TDat: An Efficient Platform for Processing Petabyte-Scale Whole-Brain Volumetric Images journal July 2017
Synchrotron Radiation Computed Tomography Station at the ESRF Biomedical Beamline
  • Nemoz, C.; Bayat, S.; Berruyer, G.
  • SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation Instrumentation, AIP Conference Proceedings https://doi.org/10.1063/1.2436440
conference January 2007
Image stitching strategies for tomographic imaging of large objects at high resolution at synchrotron sources
  • Kyrieleis, A.; Ibison, M.; Titarenko, V.
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 607, Issue 3 https://doi.org/10.1016/j.nima.2009.06.030
journal August 2009
A multi-resolution approach to the 3D reconstruction from an electron microscope tilt series solving the alignment problem without gold particles journal July 1989
Developments in synchrotron x-ray computed microtomography at the National Synchrotron Light Source
  • Dowd, Betsy A.; Campbell, Graham H.; Marr, Robert B.
  • SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, SPIE Proceedings https://doi.org/10.1117/12.363725
conference September 1999
High-resolution whole-brain staining for electron microscopic circuit reconstruction journal April 2015
Reliable method for calculating the center of rotation in parallel-beam tomography journal January 2014
Parallel distributed computing using Python journal September 2011
V3D enables real-time 3D visualization and quantitative analysis of large-scale biological image data sets journal March 2010
On local tomography journal June 1995
Automated determination of the center of rotation in tomography data journal January 2006
ParaView: An End-User Tool for Large-Data Visualization book January 2005
Rapid alignment of nanotomography data using joint iterative reconstruction and reprojection journal September 2017
Integration of TomoPy and the ASTRA toolbox for advanced processing and reconstruction of tomographic synchrotron data journal April 2016