skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Identification of micro-organisms using superconducting tunnel junctions and time-of-flight mass spectrometry

Abstract

We present time-of-flight measurements of biological material ejected from complete bacterial spores following laser irradiation. Ion impacts are registered on a microchannel plate and on a Superconducting Tunnel Junction (STJ) detector. We compare mass spectra obtained with the two detectors. The STJ has better sensitivity to massive ions and also measures the energy of each ion. We show evidence that spores of different bacillus species produce distinctive mass spectra and associate the observed mass peaks with coat proteins identified through ion-exchange extraction and gel electrophoresis.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab., CA (US)
Sponsoring Org.:
USDOE Office of Defense Programs (DP) (US)
OSTI Identifier:
14671
Report Number(s):
UCRL-JC-134432; YN0100000
YN0100000; TRN: AH200129%%378
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Eighth International Workshop on Low Temperature Detectors LTD-8, Dalfsen (NL), 08/15/1999--08/20/1999; Other Information: PBD: 30 Aug 1999
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; BACILLUS; BACTERIAL SPORES; BIOLOGICAL MATERIALS; ELECTROPHORESIS; ION EXCHANGE; IRRADIATION; LASER RADIATION; MASS SPECTRA; PROTEINS; SENSITIVITY; TIME-OF-FLIGHT MASS SPECTROMETERS; SUPERCONDUCTING JUNCTIONS

Citation Formats

Benner, W H, Frank, M, Horn, J M, Labov, S E, Langry, K, and Ullom, J. Identification of micro-organisms using superconducting tunnel junctions and time-of-flight mass spectrometry. United States: N. p., 1999. Web.
Benner, W H, Frank, M, Horn, J M, Labov, S E, Langry, K, & Ullom, J. Identification of micro-organisms using superconducting tunnel junctions and time-of-flight mass spectrometry. United States.
Benner, W H, Frank, M, Horn, J M, Labov, S E, Langry, K, and Ullom, J. Mon . "Identification of micro-organisms using superconducting tunnel junctions and time-of-flight mass spectrometry". United States. https://www.osti.gov/servlets/purl/14671.
@article{osti_14671,
title = {Identification of micro-organisms using superconducting tunnel junctions and time-of-flight mass spectrometry},
author = {Benner, W H and Frank, M and Horn, J M and Labov, S E and Langry, K and Ullom, J},
abstractNote = {We present time-of-flight measurements of biological material ejected from complete bacterial spores following laser irradiation. Ion impacts are registered on a microchannel plate and on a Superconducting Tunnel Junction (STJ) detector. We compare mass spectra obtained with the two detectors. The STJ has better sensitivity to massive ions and also measures the energy of each ion. We show evidence that spores of different bacillus species produce distinctive mass spectra and associate the observed mass peaks with coat proteins identified through ion-exchange extraction and gel electrophoresis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {8}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: