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Title: A Prospective Study on the Fermentation Landscape of Gaseous Substrates to Biorenewables Using Methanosarcina acetivorans Metabolic Model

Abstract

The abundance of methane in shale gas and of other gases such as carbon monoxide, hydrogen, and carbon dioxide as chemical process byproducts has motivated the use of gas fermentation for bioproduction. Recent advances in metabolic engineering and synthetic biology allow for engineering of microbes metabolizing a variety of chemicals including gaseous feeds into a number of biorenewables and transportation liquid fuels. New computational tools enable the systematic exploration of all feasible conversion alternatives. Here we computationally assessed all thermodynamically feasible ways of co-utilizing CH 4, CO, and CO 2 using ferric as terminal electron acceptor for the production of all key precursor metabolites. We identified the thermodynamically feasible co-utilization ratio ranges of CH 4, CO, and CO 2 toward production of the target metabolite(s) as a function of ferric uptake. A revised version of the iMAC868 genome-scale metabolic model of Methanosarcina acetivorans was chosen to assess co-utilization of CH 4, CO, and CO 2 and their conversion into selected target products using the optStoic pathway design tool. This revised version contains the latest information on electron flow mechanisms by the methanogen while supplied with methane as the sole carbon source. The interplay between different gas co-utilization ratios and themore » energetics of reverse methanogenesis were also analyzed using the same metabolic model.« less

Authors:
;
Publication Date:
Research Org.:
Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
1466420
Alternate Identifier(s):
OSTI ID: 1510462
Grant/Contract Number:  
AR0000431
Resource Type:
Journal Article: Published Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Name: Frontiers in Microbiology Journal Volume: 9; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Media SA
Country of Publication:
Switzerland
Language:
English
Subject:
09 BIOMASS FUELS; gas fermentation; metabolic modeling; CH4; CO; CO2; M. acetivorans

Citation Formats

Nazem-Bokaee, Hadi, and Maranas, Costas D. A Prospective Study on the Fermentation Landscape of Gaseous Substrates to Biorenewables Using Methanosarcina acetivorans Metabolic Model. Switzerland: N. p., 2018. Web. doi:10.3389/fmicb.2018.01855.
Nazem-Bokaee, Hadi, & Maranas, Costas D. A Prospective Study on the Fermentation Landscape of Gaseous Substrates to Biorenewables Using Methanosarcina acetivorans Metabolic Model. Switzerland. doi:10.3389/fmicb.2018.01855.
Nazem-Bokaee, Hadi, and Maranas, Costas D. Fri . "A Prospective Study on the Fermentation Landscape of Gaseous Substrates to Biorenewables Using Methanosarcina acetivorans Metabolic Model". Switzerland. doi:10.3389/fmicb.2018.01855.
@article{osti_1466420,
title = {A Prospective Study on the Fermentation Landscape of Gaseous Substrates to Biorenewables Using Methanosarcina acetivorans Metabolic Model},
author = {Nazem-Bokaee, Hadi and Maranas, Costas D.},
abstractNote = {The abundance of methane in shale gas and of other gases such as carbon monoxide, hydrogen, and carbon dioxide as chemical process byproducts has motivated the use of gas fermentation for bioproduction. Recent advances in metabolic engineering and synthetic biology allow for engineering of microbes metabolizing a variety of chemicals including gaseous feeds into a number of biorenewables and transportation liquid fuels. New computational tools enable the systematic exploration of all feasible conversion alternatives. Here we computationally assessed all thermodynamically feasible ways of co-utilizing CH4, CO, and CO2 using ferric as terminal electron acceptor for the production of all key precursor metabolites. We identified the thermodynamically feasible co-utilization ratio ranges of CH4, CO, and CO2 toward production of the target metabolite(s) as a function of ferric uptake. A revised version of the iMAC868 genome-scale metabolic model of Methanosarcina acetivorans was chosen to assess co-utilization of CH4, CO, and CO2 and their conversion into selected target products using the optStoic pathway design tool. This revised version contains the latest information on electron flow mechanisms by the methanogen while supplied with methane as the sole carbon source. The interplay between different gas co-utilization ratios and the energetics of reverse methanogenesis were also analyzed using the same metabolic model.},
doi = {10.3389/fmicb.2018.01855},
journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = ,
volume = 9,
place = {Switzerland},
year = {2018},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.3389/fmicb.2018.01855

Citation Metrics:
Cited by: 1 work
Citation information provided by
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Figures / Tables:

FIGURE 1 FIGURE 1: | Electron bifurcation mechanism by HdrA2B2C2 complex of M. acetivorans in the presence of external electron acceptor when grown with methane (see Yan et al., 2017 for more details). F420: Cofactor F420; F420H2: reduced form of cofactor F420; Fdx: ferredoxin; Fdx2−: reduced form of ferredoxin; HSCoM: coenzyme M;more » HSCoB: coenzyme B; CoM-S-S-CoB: heterodisulfide.« less

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    Works referencing / citing this record:

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      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.