Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves

Yurchenko, Olga; Shockey, Jay M.; Gidda, Satinder K.; Silver, Maxwell I.; Chapman, Kent D.; Mullen, Robert T.; Dyer, John M.

doi:10.1111/pbi.12695

Title: Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves

Journal Article · Wed Mar 15 00:00:00 EDT 2017 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12695· OSTI ID:1347058

Yurchenko, Olga ^[1]; Shockey, Jay M. ^[2]; Gidda, Satinder K. ^[3]; Silver, Maxwell I. ^[3]; Chapman, Kent D. ^[4]; Mullen, Robert T. ^[3]; Dyer, John M. ^[1]

USDA‐ARS US Arid‐Land Agricultural Research Center Maricopa AZ USA
USDA‐ARS Southern Regional Research Center New Orleans LA USA
Department of Molecular and Cellular Biology University of Guelph Guelph ON Canada
Department of Biological Sciences University of North Texas Denton TX USA

Summary The seeds of many nondomesticated plant species synthesize oils containing high amounts of a single unusual fatty acid, many of which have potential usage in industry. Despite the identification of enzymes for unusual oxidized fatty acid synthesis, the production of these fatty acids in engineered seeds remains low and is often hampered by their inefficient exclusion from phospholipids. Recent studies have established the feasibility of increasing triacylglycerol content in plant leaves, which provides a novel approach for increasing energy density of biomass crops. Here, we determined whether the fatty acid composition of leaf oil could be engineered to accumulate unusual fatty acids. Eleostearic acid ( ESA ) is a conjugated fatty acid produced in seeds of the tung tree ( Vernicia fordii ) and has both industrial and nutritional end‐uses. Arabidopsis thaliana lines with elevated leaf oil were first generated by transforming wild‐type, cgi‐58 or pxa1 mutants (the latter two of which contain mutations disrupting fatty acid breakdown) with the diacylglycerol acyltransferases ( DGAT 1 or DGAT 2 ) and/or oleosin genes from tung. High‐leaf‐oil plant lines were then transformed with tung FADX , which encodes the fatty acid desaturase/conjugase responsible for ESA synthesis. Analysis of lipids in leaves revealed that ESA was efficiently excluded from phospholipids, and co‐expression of tung FADX and DGAT 2 promoted a synergistic increase in leaf oil content and ESA accumulation. Taken together, these results provide a new approach for increasing leaf oil content that is coupled with accumulation of unusual fatty acids. Implications for production of biofuels, bioproducts, and plant–pest interactions are discussed.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: DE‐FG02‐09ER64812; DE‐SC0016536; SC0016536

OSTI ID:: 1347058

Alternate ID(s):: OSTI ID: 1347059; OSTI ID: 1465812

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 15 Journal Issue: 8; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 25 works

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
conjugated fatty acids
bio‐based feedstocks
biofuels
oil in leaves
Vernicia fordii

Title: Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves

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