Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRY1

Journal Article · Fri Aug 18 00:00:00 EDT 1995 · Science

DOI:https://doi.org/10.1126/science.7638620· OSTI ID:146535

Lin, C; Robertson, D E; Ahmad, M ^[1]

---

1. Univ. of Pennsylvania, Philadelphia, PA (United States); and others

The Arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH{center_dot}). The absorption properties of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum. Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity. 27 refs., 4 figs.

• Sponsoring Organization: USDOE
• DOE Contract Number: FG02-87ER13680
• OSTI ID: 146535
• Journal Information: Science, Vol. 269, Issue 5226; Other Information: PBD: 18 Aug 1995
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ARABIDOPSIS
PLANT GROWTH
RESPONSE MODIFYING FACTORS
RECEPTORS
VISIBLE RADIATION
ABSORPTION
NUCLEOTIDES