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Title: Simultaneous Quantification of the Acetylome and Succinylome by ‘One-Pot’ Affinity Enrichment

Abstract

Protein posttranslational modifications (PTMs) are of increasing interest in biomedical research, yet studies rarely examine more than one PTM. One barrier to multi-PTM studies is the time cost for both sample preparation and data acquisition, which scale linearly with the number of modifications. The most prohibitive requirement is often the need for large amounts of sample, which must be increased proportionally with the number of PTM enrichment steps. Here, a streamlined, quantitative label-free proteomic workflow—“one-pot” PTM enrichment—that enables comprehensive identification and quantification of peptides containing acetylated and succinylated lysine residues from a single sample containing as little as 1 mg mitochondria protein is described. Coupled with a label-free, data-independent acquisition (DIA), 2235 acetylated and 2173 succinylated peptides with the one-pot method are identified and quantified and peak areas are shown to be highly correlated between the one-pot and traditional single-PTM enrichments. The ‘one-pot’ method makes possible detection of multiple PTMs occurring on the same peptide, and it is shown that it can be used to make unique biological insights into PTM crosstalk. Compared to single-PTM enrichments, the one-pot workflow has equivalent reproducibility and enables direct assessment of PTM crosstalk from biological samples in less time from less tissue.

Authors:
 [1];  [1];  [1];  [2];  [1]
  1. The Buck Institute for Research on Aging, 94945 Novato CA USA
  2. The Buck Institute for Research on Aging, 94945 Novato CA USA, Amgen, 94080 South San Francisco CA USA
Publication Date:
Research Org.:
Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1465215
Alternate Identifier(s):
OSTI ID: 1465216; OSTI ID: 1545800
Grant/Contract Number:  
SC0012443; OD016281
Resource Type:
Journal Article: Published Article
Journal Name:
Proteomics
Additional Journal Information:
Journal Name: Proteomics Journal Volume: 18 Journal Issue: 17; Journal ID: ISSN 1615-9853
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Germany
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; acetylation; data‐independent acquisition; immunoaffinity enrichment; posttranslational modification; succinylation

Citation Formats

Basisty, Nathan, Meyer, Jesse G., Wei, Lei, Gibson, Bradford W., and Schilling, Birgit. Simultaneous Quantification of the Acetylome and Succinylome by ‘One-Pot’ Affinity Enrichment. Germany: N. p., 2018. Web. doi:10.1002/pmic.201800123.
Basisty, Nathan, Meyer, Jesse G., Wei, Lei, Gibson, Bradford W., & Schilling, Birgit. Simultaneous Quantification of the Acetylome and Succinylome by ‘One-Pot’ Affinity Enrichment. Germany. https://doi.org/10.1002/pmic.201800123
Basisty, Nathan, Meyer, Jesse G., Wei, Lei, Gibson, Bradford W., and Schilling, Birgit. Sun . "Simultaneous Quantification of the Acetylome and Succinylome by ‘One-Pot’ Affinity Enrichment". Germany. https://doi.org/10.1002/pmic.201800123.
@article{osti_1465215,
title = {Simultaneous Quantification of the Acetylome and Succinylome by ‘One-Pot’ Affinity Enrichment},
author = {Basisty, Nathan and Meyer, Jesse G. and Wei, Lei and Gibson, Bradford W. and Schilling, Birgit},
abstractNote = {Protein posttranslational modifications (PTMs) are of increasing interest in biomedical research, yet studies rarely examine more than one PTM. One barrier to multi-PTM studies is the time cost for both sample preparation and data acquisition, which scale linearly with the number of modifications. The most prohibitive requirement is often the need for large amounts of sample, which must be increased proportionally with the number of PTM enrichment steps. Here, a streamlined, quantitative label-free proteomic workflow—“one-pot” PTM enrichment—that enables comprehensive identification and quantification of peptides containing acetylated and succinylated lysine residues from a single sample containing as little as 1 mg mitochondria protein is described. Coupled with a label-free, data-independent acquisition (DIA), 2235 acetylated and 2173 succinylated peptides with the one-pot method are identified and quantified and peak areas are shown to be highly correlated between the one-pot and traditional single-PTM enrichments. The ‘one-pot’ method makes possible detection of multiple PTMs occurring on the same peptide, and it is shown that it can be used to make unique biological insights into PTM crosstalk. Compared to single-PTM enrichments, the one-pot workflow has equivalent reproducibility and enables direct assessment of PTM crosstalk from biological samples in less time from less tissue.},
doi = {10.1002/pmic.201800123},
url = {https://www.osti.gov/biblio/1465215}, journal = {Proteomics},
issn = {1615-9853},
number = 17,
volume = 18,
place = {Germany},
year = {2018},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1002/pmic.201800123

Citation Metrics:
Cited by: 5 works
Citation information provided by
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Works referenced in this record:

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    Works referencing / citing this record:

    Nanomaterials in Proteomics
    journal, March 2019


    Global Lysine Acetylation in Escherichia coli Results from Growth Conditions That Favor Acetate Fermentation
    journal, February 2019


    Mechanisms, Detection, and Relevance of Protein Acetylation in Prokaryotes
    journal, April 2019


    Proteomics: a powerful tool to study plant responses to biotic stress
    journal, November 2019


    Post-translational Protein Acetylation: An Elegant Mechanism for Bacteria to Dynamically Regulate Metabolic Functions
    journal, July 2019