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Title: Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface

Abstract

Here, the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. In conclusion, this immediate drop on demand technology/open port sampling interface/ESI–MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Dispendix GmbH, Stuttgart (Germany)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1465070
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Bioanalysis
Additional Journal Information:
Journal Volume: 9; Journal Issue: 21; Journal ID: ISSN 1757-6180
Publisher:
Future Science Group
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; drop dispensing; electrospray ionization; immediate drop on demand; mass spectrometry; microtiter plates; noncontact dispensing; open port sampling interface

Citation Formats

Van Berkel, Gary J., Kertesz, Vilmos, and Boeltz, Harry. Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface. United States: N. p., 2017. Web. doi:10.4155/bio-2017-0104.
Van Berkel, Gary J., Kertesz, Vilmos, & Boeltz, Harry. Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface. United States. https://doi.org/10.4155/bio-2017-0104
Van Berkel, Gary J., Kertesz, Vilmos, and Boeltz, Harry. Thu . "Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface". United States. https://doi.org/10.4155/bio-2017-0104. https://www.osti.gov/servlets/purl/1465070.
@article{osti_1465070,
title = {Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface},
author = {Van Berkel, Gary J. and Kertesz, Vilmos and Boeltz, Harry},
abstractNote = {Here, the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. In conclusion, this immediate drop on demand technology/open port sampling interface/ESI–MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.},
doi = {10.4155/bio-2017-0104},
url = {https://www.osti.gov/biblio/1465070}, journal = {Bioanalysis},
issn = {1757-6180},
number = 21,
volume = 9,
place = {United States},
year = {2017},
month = {11}
}

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Free Publicly Available Full Text
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Cited by: 3 works
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Works referenced in this record:

Induction based fluidics (IBF) for droplet-based mass spectrometric analysis of oligonucleotides: IBF mass spectrometry of oligonucleotides
journal, September 2015


A universal algorithm for fast and automated charge state deconvolution of electrospray mass-to-charge ratio spectra
journal, March 1998


High-Throughput Mode Liquid Microjunction Surface Sampling Probe
journal, August 2009


Technologies and Applications in Micro-Volume Liquid Handling
journal, January 2016


Drugs of abuse in drinking water – a review of current detection methods, occurrence, elimination and health risks
journal, December 2016


An open port sampling interface for liquid introduction atmospheric pressure ionization mass spectrometry: Open port sampling interface
journal, August 2015


Laser dissection sampling modes for direct mass spectral analysis: Laser dissection sampling modes for mass spectral analysis
journal, February 2016


Contaminants in water: non-target UHPLC/MS analysis
journal, August 2015


Automated High-Throughput Infusion ESI-MS with Direct Coupling to a Microtiter Plate
journal, April 2001


Lead discovery for mammalian elongation of long chain fatty acids family 6 using a combination of high-throughput fluorescent-based assay and RapidFire mass spectrometry assay
journal, November 2016


Transmission geometry laser ablation into a non-contact liquid vortex capture probe for mass spectrometry imaging
journal, June 2014

  • Ovchinnikova, Olga S.; Bhandari, Deepak; Lorenz, Matthias
  • Rapid Communications in Mass Spectrometry, Vol. 28, Issue 15, p. 1665-1673
  • https://doi.org/10.1002/rcm.6946

Cell Dispensing in Low-Volume Range with the Immediate Drop-on-Demand Technology (I-DOT)
journal, April 2015


Development of Remote Sampling ESI Mass Spectrometry for the Rapid and Automatic Analysis of Multiple Samples
journal, January 2017


New technique for needle-less implantation of eukaryotic cells
journal, November 2015


Novel Acoustic Loading of a Mass Spectrometer: Toward Next-Generation High-Throughput MS Screening
journal, February 2016


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