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Title: Immobilisation of Platinum by Cupriavidus metallidurans

Abstract

The metal resistant bacterium Cupriavidus metallidurans CH34, challenged with aqueous platinous and platinic chloride, rapidly immobilized platinum. XANES/EXAFS analysis of these reaction systems demonstrated that platinum binding shifted from chloride to carboxyl functional groups within the bacteria. Pt(IV) was more toxic than Pt(II), presumably due to the oxidative stress imparted by the platinic form. Platinum immobilisation increased with time and with increasing concentrations of platinum. From a bacterial perspective, intracellular platinum concentrations were two to three orders of magnitude greater than the fluid phase, and became saturated at almost molar concentrations in both reaction systems. TEM revealed that C. metallidurans was also able to precipitate nm-scale colloidal platinum, primarily along the cell envelope where energy generation/electron transport occurs. Cells enriched in platinum shed outer membrane vesicles that were enriched in metallic, colloidal platinum, likely representing an important detoxification strategy. Furthermore, the formation of organo-platinum compounds and membrane encapsulated nanophase platinum, supports a role for bacteria in the formation and transport of platinum in natural systems, forming dispersion halos important to metal exploration.

Authors:
 [1];  [2];  [3];  [4];  [4];  [5]
  1. The Univ. of Western Ontario, London, ON (Canada)
  2. Canadian Light Source, Saskatoon, SK (Canada)
  3. The Univ. of Adelaide, Adelaide, SA (Australia); CSIRO Land and Water, Glen Osmond, SA (Australia)
  4. Pacific Northwest Consortium Synchrotron Radiation Facility, Argonne, IL (United States)
  5. The Univ. of Queensland, St. Lucia (Australia)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
Australian Research Council; Natural Sciences and Engineering Research Council of Canada (NSERC); USDOE
OSTI Identifier:
1464755
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Minerals
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2075-163X
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Cupriavidus metallidurans; biomineralisation; geomicrobiology; platinum; synchrotron

Citation Formats

Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., and Southam, Gordon. Immobilisation of Platinum by Cupriavidus metallidurans. United States: N. p., 2018. Web. doi:10.3390/min8010010.
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., & Southam, Gordon. Immobilisation of Platinum by Cupriavidus metallidurans. United States. https://doi.org/10.3390/min8010010
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., and Southam, Gordon. Fri . "Immobilisation of Platinum by Cupriavidus metallidurans". United States. https://doi.org/10.3390/min8010010. https://www.osti.gov/servlets/purl/1464755.
@article{osti_1464755,
title = {Immobilisation of Platinum by Cupriavidus metallidurans},
author = {Campbell, Gordon and MacLean, Lachlan and Reith, Frank and Brewe, Dale and Gordon, Robert A. and Southam, Gordon},
abstractNote = {The metal resistant bacterium Cupriavidus metallidurans CH34, challenged with aqueous platinous and platinic chloride, rapidly immobilized platinum. XANES/EXAFS analysis of these reaction systems demonstrated that platinum binding shifted from chloride to carboxyl functional groups within the bacteria. Pt(IV) was more toxic than Pt(II), presumably due to the oxidative stress imparted by the platinic form. Platinum immobilisation increased with time and with increasing concentrations of platinum. From a bacterial perspective, intracellular platinum concentrations were two to three orders of magnitude greater than the fluid phase, and became saturated at almost molar concentrations in both reaction systems. TEM revealed that C. metallidurans was also able to precipitate nm-scale colloidal platinum, primarily along the cell envelope where energy generation/electron transport occurs. Cells enriched in platinum shed outer membrane vesicles that were enriched in metallic, colloidal platinum, likely representing an important detoxification strategy. Furthermore, the formation of organo-platinum compounds and membrane encapsulated nanophase platinum, supports a role for bacteria in the formation and transport of platinum in natural systems, forming dispersion halos important to metal exploration.},
doi = {10.3390/min8010010},
url = {https://www.osti.gov/biblio/1464755}, journal = {Minerals},
issn = {2075-163X},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {1}
}

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Cited by: 8 works
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    Works referencing / citing this record:

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    journal, September 2018


    Editorial for Special Issue “Geomicrobiology and Biogeochemistry of Precious Metals”
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