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Title: A plastidial pantoate transporter with a potential role in pantothenate synthesis

Abstract

The pantothenate (vitamin B5) synthesis pathway in plants is not fully defined because the subcellular site of its ketopantoate → pantoate reduction step is unclear. However, the pathway is known to be split between cytosol, mitochondria, and potentially plastids, and inferred to involve mitochondrial or plastidial transport of ketopantoate or pantoate. No proteins that mediate these transport steps have been identified. Comparative genomic and transcriptomic analyses identified Arabidopsis thaliana BASS1 (At1g78560) and its maize (Zea mays) ortholog as candidates for such a transport role. BASS1 proteins belong to the bile acid:sodium symporter family and share similarity with the Salmonella enterica PanS pantoate/ketopantoate transporter and with predicted bacterial transporters whose genes cluster on the chromosome with pantothenate synthesis genes. Furthermore, Arabidopsis BASS1 is co-expressed with genes related to metabolism of coenzyme A, the cofactor derived from pantothenate. Expression of Arabidopsis or maize BASS1 promoted growth of a S. enterica panB panS mutant strain when pantoate but not ketopantoate was supplied, and increased the rate of [3H]pantoate uptake. Subcellular localization of green fluorescent protein fusions in Nicotiana tabacum BY-2 cells demonstrated that Arabidopsis BASS1 is targeted solely to the plastid inner envelope. Two independent Arabidopsis BASS1 knockout mutants accumulated pantoate about tenfoldmore » in leaves and had smaller seeds. Together, these data indicate that BASS1 is a physiologically significant plastidial pantoate transporter and that the pantoate reduction step in pantothenate biosynthesis could be at least partly localized in plastids.« less

Authors:
; ; ; ; ; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Science Foundation (NSF); Natural Sciences and Engineering Research Council of Canada (NSERC)
OSTI Identifier:
1464642
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Biochemical Journal
Additional Journal Information:
Journal Volume: 475; Journal Issue: 4; Journal ID: ISSN 0264-6021
Publisher:
Biochemical Society
Country of Publication:
United States
Language:
English
Subject:
coenzyme A; green fluorescent protein; pantothenate; salmonella enterica; vitamin B5

Citation Formats

Huang, Lili, Pyc, Michal, Alseekh, Saleh, McCarty, Donald R., de Crécy-Lagard, Valérie, Gregory, Jesse F., Henry, Christopher S., Fernie, Alisdair R., Mullen, Robert T., and Hanson, Andrew D. A plastidial pantoate transporter with a potential role in pantothenate synthesis. United States: N. p., 2018. Web. doi:10.1042/BCJ20170883.
Huang, Lili, Pyc, Michal, Alseekh, Saleh, McCarty, Donald R., de Crécy-Lagard, Valérie, Gregory, Jesse F., Henry, Christopher S., Fernie, Alisdair R., Mullen, Robert T., & Hanson, Andrew D. A plastidial pantoate transporter with a potential role in pantothenate synthesis. United States. doi:10.1042/BCJ20170883.
Huang, Lili, Pyc, Michal, Alseekh, Saleh, McCarty, Donald R., de Crécy-Lagard, Valérie, Gregory, Jesse F., Henry, Christopher S., Fernie, Alisdair R., Mullen, Robert T., and Hanson, Andrew D. Tue . "A plastidial pantoate transporter with a potential role in pantothenate synthesis". United States. doi:10.1042/BCJ20170883.
@article{osti_1464642,
title = {A plastidial pantoate transporter with a potential role in pantothenate synthesis},
author = {Huang, Lili and Pyc, Michal and Alseekh, Saleh and McCarty, Donald R. and de Crécy-Lagard, Valérie and Gregory, Jesse F. and Henry, Christopher S. and Fernie, Alisdair R. and Mullen, Robert T. and Hanson, Andrew D.},
abstractNote = {The pantothenate (vitamin B5) synthesis pathway in plants is not fully defined because the subcellular site of its ketopantoate → pantoate reduction step is unclear. However, the pathway is known to be split between cytosol, mitochondria, and potentially plastids, and inferred to involve mitochondrial or plastidial transport of ketopantoate or pantoate. No proteins that mediate these transport steps have been identified. Comparative genomic and transcriptomic analyses identified Arabidopsis thaliana BASS1 (At1g78560) and its maize (Zea mays) ortholog as candidates for such a transport role. BASS1 proteins belong to the bile acid:sodium symporter family and share similarity with the Salmonella enterica PanS pantoate/ketopantoate transporter and with predicted bacterial transporters whose genes cluster on the chromosome with pantothenate synthesis genes. Furthermore, Arabidopsis BASS1 is co-expressed with genes related to metabolism of coenzyme A, the cofactor derived from pantothenate. Expression of Arabidopsis or maize BASS1 promoted growth of a S. enterica panB panS mutant strain when pantoate but not ketopantoate was supplied, and increased the rate of [3H]pantoate uptake. Subcellular localization of green fluorescent protein fusions in Nicotiana tabacum BY-2 cells demonstrated that Arabidopsis BASS1 is targeted solely to the plastid inner envelope. Two independent Arabidopsis BASS1 knockout mutants accumulated pantoate about tenfold in leaves and had smaller seeds. Together, these data indicate that BASS1 is a physiologically significant plastidial pantoate transporter and that the pantoate reduction step in pantothenate biosynthesis could be at least partly localized in plastids.},
doi = {10.1042/BCJ20170883},
journal = {Biochemical Journal},
issn = {0264-6021},
number = 4,
volume = 475,
place = {United States},
year = {2018},
month = {1}
}

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