Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls

Ye, Dan; Kiemle, Sarah N.; Rongpipi, Sintu; Wang, Xuan; Wang, Cheng; Cosgrove, Daniel J.; Gomez, Esther W.; Gomez, Enrique D.

doi:10.1038/s41598-018-31024-1

Title: Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls

Journal Article · Mon Aug 20 00:00:00 EDT 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-31024-1· OSTI ID:1464571

Ye, Dan; Kiemle, Sarah N.;

; Wang, Xuan;

; Cosgrove, Daniel J.; Gomez, Esther W.;

Cellulose microfibrils are crucial for many of the remarkable mechanical properties of primary cell walls. Nevertheless, many structural features of cellulose microfibril organization in cell walls are not yet fully described. Microscopy techniques provide direct visualization of cell wall organization, and quantification of some aspects of wall microstructure is possible through image processing. Complementary to microscopy techniques, scattering yields structural information in reciprocal space over large sample areas. Using the onion epidermal wall as a model system, we introduce resonant soft X-ray scattering (RSoXS) to directly quantify the average interfibril spacing. Tuning the X-ray energy to the calcium L-edge enhances the contrast between cellulose and pectin due to the localization of calcium ions to homogalacturonan in the pectin matrix. As a consequence, RSoXS profiles reveal an average center-to-center distance between cellulose microfibrils or microfibril bundles of about 20 nm.

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Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231; SC0001090

OSTI ID:: 1464571

Alternate ID(s):: OSTI ID: 1477410; OSTI ID: 1631157

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 8 Journal Issue: 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 22 works

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