skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids

Abstract

Here, the ability to manipulate expression of key biosynthetic enzymes has allowed the development of genetically modified plants that synthesise unusual lipids that are useful for biofuel and industrial applications. By taking advantage of the unique activities of enzymes from different species, tailored lipids with a targeted structure can be conceived. In this study we demonstrate the successful implementation of such an approach by metabolically engineering the oilseed crop Camelina sativa to produce 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) with medium-chain fatty acids (MCFAs). Different transgenic camelina lines that had been genetically modified to produce MCFAs through the expression of MCFA-specific thioesterases and acyltransferases were retransformed with the Euonymus alatus gene for diacylglycerol acetyltransferase (EaDAcT) that synthesises acetyl-TAGs. Concomitant RNAi suppression of acyl-CoA:diacylglycerol acyltransferase increased the levels of acetyl-TAG, with up to 77 mole percent in the best lines. However, the total oil content was reduced. Analysis of the composition of the acetyl-TAG molecular species using electrospray ionisation mass spectrometry demonstrated the successful synthesis of acetyl-TAG containing MCFAs. Field growth of high-yielding plants generated enough oil for quantification of viscosity. As part of an ongoing design–test–learn cycle, these results, which include not only the synthesis of ‘designer’ lipids but also their functional analysis, willmore » lead to the future production of such molecules tailored for specific applications.« less

Authors:
 [1];  [2];  [3];  [4];  [2];  [3];  [1]
  1. Kansas State Univ., Manhattan, KS (United States)
  2. Univ. of Nebraska, Lincoln, NE (United States)
  3. Montana State Univ., Bozeman, MT (United States)
  4. Department of Chemistry and Biology, Bethany College, Lindsborg, KS (United States)
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1464521
Grant/Contract Number:  
SC0018420
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 69; Journal Issue: 18; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Acetyl-TAG; biofuels; bioproducts; Camelina sativa; medium chain fatty acid; synthetic biology; vegetable oil

Citation Formats

Bansal, Sunil, Kim, Hae Jin, Na, GunNam, Hamilton, Megan E., Cahoon, Edgar B., Lu, Chaofu, and Durrett, Timothy P. Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids. United States: N. p., 2018. Web. doi:10.1093/jxb/ery225.
Bansal, Sunil, Kim, Hae Jin, Na, GunNam, Hamilton, Megan E., Cahoon, Edgar B., Lu, Chaofu, & Durrett, Timothy P. Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids. United States. doi:10.1093/jxb/ery225.
Bansal, Sunil, Kim, Hae Jin, Na, GunNam, Hamilton, Megan E., Cahoon, Edgar B., Lu, Chaofu, and Durrett, Timothy P. Fri . "Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids". United States. doi:10.1093/jxb/ery225. https://www.osti.gov/servlets/purl/1464521.
@article{osti_1464521,
title = {Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids},
author = {Bansal, Sunil and Kim, Hae Jin and Na, GunNam and Hamilton, Megan E. and Cahoon, Edgar B. and Lu, Chaofu and Durrett, Timothy P.},
abstractNote = {Here, the ability to manipulate expression of key biosynthetic enzymes has allowed the development of genetically modified plants that synthesise unusual lipids that are useful for biofuel and industrial applications. By taking advantage of the unique activities of enzymes from different species, tailored lipids with a targeted structure can be conceived. In this study we demonstrate the successful implementation of such an approach by metabolically engineering the oilseed crop Camelina sativa to produce 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) with medium-chain fatty acids (MCFAs). Different transgenic camelina lines that had been genetically modified to produce MCFAs through the expression of MCFA-specific thioesterases and acyltransferases were retransformed with the Euonymus alatus gene for diacylglycerol acetyltransferase (EaDAcT) that synthesises acetyl-TAGs. Concomitant RNAi suppression of acyl-CoA:diacylglycerol acyltransferase increased the levels of acetyl-TAG, with up to 77 mole percent in the best lines. However, the total oil content was reduced. Analysis of the composition of the acetyl-TAG molecular species using electrospray ionisation mass spectrometry demonstrated the successful synthesis of acetyl-TAG containing MCFAs. Field growth of high-yielding plants generated enough oil for quantification of viscosity. As part of an ongoing design–test–learn cycle, these results, which include not only the synthesis of ‘designer’ lipids but also their functional analysis, will lead to the future production of such molecules tailored for specific applications.},
doi = {10.1093/jxb/ery225},
journal = {Journal of Experimental Botany},
issn = {0022-0957},
number = 18,
volume = 69,
place = {United States},
year = {2018},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share: