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Title: Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress

Abstract

Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2 → 6)-β-d-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6T, in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48 h. The antiproliferative activity was analyzed by the MTT assay. Oxidative stress was measured by the CM-H2DCFDA assay, and cell apoptosis was analyzed by the caspase-3/7 assay. Cell cycle was analyzed by flow cytometry and gene expression was determined by RT-PCR. Levan showed a time- and concentration-dependent antiproliferative activity, and this effect was associated with an increase in cell apoptosis and oxidative stress. In addition, levan increased the gene expression of p53 and p27. Here we demonstrated that levan exhibited an antiproliferative effect that was mediated by an increase in apoptosis and oxidative stress.

Authors:
 [1];  [2];  [3];  [4];  [5];  [4];  [6];  [7]
  1. Universidade de São Paulo, SP (Brazil). Departamento de Farmacologia, Instituto de Ciências Biomédicas; Lakehead University, Thunder Bay, ON (Canada). Biorefining Research Institute; Lakehead University, Thunder Bay, ON (Canada). Medical Sciences Division, Northern Ontario School of Medicine (NOSM); Universidade Federal de Mato Grosso, Sinop, MT (Brazil). Unidade de Pesquisa Experimental, Instituto de Ciências da Saúde
  2. Universidade de São Paulo, SP (Brazil). Departamento de Farmacologia, Instituto de Ciências Biomédicas
  3. Universidade Tecnológica Federal do Paraná, Pato Branco, SP (Brazil). Departamento de Química
  4. Marmara University, Goztepe, Istanbul (Turkey). IBSB, Department of Bioengineering
  5. Lakehead University, Thunder Bay, ON (Canada). Biorefining Research Institute; Universidade Estadual de Londrina, CCE, Londrina, PR (Brazil). Departamento de Química
  6. Lakehead University, Thunder Bay, ON (Canada). Biorefining Research Institute; Universidade Tecnológica Federal do Paraná, Campus Londrina, Londrina, PR (Brazil). Programa de Pós-Graduação em Engenharia Ambiental
  7. Lakehead University, Thunder Bay, ON (Canada). Medical Sciences Division, Northern Ontario School of Medicine (NOSM)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory, Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC).
Sponsoring Org.:
USDOE
OSTI Identifier:
1463308
Resource Type:
Journal Article
Journal Name:
International Journal of Biological Macromolecules
Additional Journal Information:
Journal Volume: 102; Journal Issue: C; Journal ID: ISSN 0141-8130
Country of Publication:
United States
Language:
English

Citation Formats

Queiroz, Eveline A. I. F., Fortes, Zuleica B., da Cunha, M�rio A. A., Sarilmiser, Hande Kazak, Barbosa Dekker, Aneli M., �ner, Ebru Toksoy, Dekker, Robert F. H., and Khaper, Neelam. Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress. United States: N. p., 2017. Web. doi:10.1016/j.ijbiomac.2017.04.035.
Queiroz, Eveline A. I. F., Fortes, Zuleica B., da Cunha, M�rio A. A., Sarilmiser, Hande Kazak, Barbosa Dekker, Aneli M., �ner, Ebru Toksoy, Dekker, Robert F. H., & Khaper, Neelam. Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress. United States. doi:10.1016/j.ijbiomac.2017.04.035.
Queiroz, Eveline A. I. F., Fortes, Zuleica B., da Cunha, M�rio A. A., Sarilmiser, Hande Kazak, Barbosa Dekker, Aneli M., �ner, Ebru Toksoy, Dekker, Robert F. H., and Khaper, Neelam. Fri . "Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress". United States. doi:10.1016/j.ijbiomac.2017.04.035.
@article{osti_1463308,
title = {Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress},
author = {Queiroz, Eveline A. I. F. and Fortes, Zuleica B. and da Cunha, M�rio A. A. and Sarilmiser, Hande Kazak and Barbosa Dekker, Aneli M. and �ner, Ebru Toksoy and Dekker, Robert F. H. and Khaper, Neelam},
abstractNote = {Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2 → 6)-β-d-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6T, in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48 h. The antiproliferative activity was analyzed by the MTT assay. Oxidative stress was measured by the CM-H2DCFDA assay, and cell apoptosis was analyzed by the caspase-3/7 assay. Cell cycle was analyzed by flow cytometry and gene expression was determined by RT-PCR. Levan showed a time- and concentration-dependent antiproliferative activity, and this effect was associated with an increase in cell apoptosis and oxidative stress. In addition, levan increased the gene expression of p53 and p27. Here we demonstrated that levan exhibited an antiproliferative effect that was mediated by an increase in apoptosis and oxidative stress.},
doi = {10.1016/j.ijbiomac.2017.04.035},
journal = {International Journal of Biological Macromolecules},
issn = {0141-8130},
number = C,
volume = 102,
place = {United States},
year = {2017},
month = {9}
}