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Title: Particle tracking by repetitive phase-shift interferometric super resolution microscopy

Abstract

Accurate and rapid particle tracking is essential for address-ing many research problems in single molecule and cellular biophysics and colloidal soft condensed matter physics. We developed a novel three-dimensional interferometric fluo-rescent particle tracking approach that does not require any sample scanning. By periodically shifting the interfer-ometer phase, the information stored in the interference pattern of the emitted light allows localizing particles posi-tions with nanometer resolution. This tracking protocol was demonstrated by measuring a known trajectory of a fluorescent bead with sub-5 nm axial localization error at 5 Hz. The interferometric microscopy was used to track the RecA protein in Bacillus subtilis bacteria to demonstrate its compatibility with biological systems.

Authors:
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Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science - Office of Biological and Environmental Research
OSTI Identifier:
1462734
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Optics Letters
Additional Journal Information:
Journal Volume: 43; Journal Issue: 12; Journal ID: ISSN 0146-9592
Publisher:
Optical Society of America (OSA)
Country of Publication:
United States
Language:
English
Subject:
fluorescence microscopy; fringe analysis; interferometry; microscopy; optical instruments; phase modulation

Citation Formats

Gdor, Itay, Wang, Xiaolei, Daddysman, Matthew, Yifat, Yuval, Wilton, Rosemarie, Hereld, Mark, Noirot-Gros, Marie-Françoise, and Scherer, Norbert F. Particle tracking by repetitive phase-shift interferometric super resolution microscopy. United States: N. p., 2018. Web. doi:10.1364/OL.43.002819.
Gdor, Itay, Wang, Xiaolei, Daddysman, Matthew, Yifat, Yuval, Wilton, Rosemarie, Hereld, Mark, Noirot-Gros, Marie-Françoise, & Scherer, Norbert F. Particle tracking by repetitive phase-shift interferometric super resolution microscopy. United States. doi:10.1364/OL.43.002819.
Gdor, Itay, Wang, Xiaolei, Daddysman, Matthew, Yifat, Yuval, Wilton, Rosemarie, Hereld, Mark, Noirot-Gros, Marie-Françoise, and Scherer, Norbert F. Mon . "Particle tracking by repetitive phase-shift interferometric super resolution microscopy". United States. doi:10.1364/OL.43.002819.
@article{osti_1462734,
title = {Particle tracking by repetitive phase-shift interferometric super resolution microscopy},
author = {Gdor, Itay and Wang, Xiaolei and Daddysman, Matthew and Yifat, Yuval and Wilton, Rosemarie and Hereld, Mark and Noirot-Gros, Marie-Françoise and Scherer, Norbert F.},
abstractNote = {Accurate and rapid particle tracking is essential for address-ing many research problems in single molecule and cellular biophysics and colloidal soft condensed matter physics. We developed a novel three-dimensional interferometric fluo-rescent particle tracking approach that does not require any sample scanning. By periodically shifting the interfer-ometer phase, the information stored in the interference pattern of the emitted light allows localizing particles posi-tions with nanometer resolution. This tracking protocol was demonstrated by measuring a known trajectory of a fluorescent bead with sub-5 nm axial localization error at 5 Hz. The interferometric microscopy was used to track the RecA protein in Bacillus subtilis bacteria to demonstrate its compatibility with biological systems.},
doi = {10.1364/OL.43.002819},
journal = {Optics Letters},
issn = {0146-9592},
number = 12,
volume = 43,
place = {United States},
year = {2018},
month = {1}
}