Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators

Juárez, Javier F.; Lecube-Azpeitia, Begoña; Brown, Stuart L.; Johnston, Christopher D.; Church, George M.

doi:10.1038/s41467-018-05525-6

Title: Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators

Journal Article · Mon Aug 06 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-05525-6· OSTI ID:1462285

Juárez, Javier F.; Lecube-Azpeitia, Begoña; Brown, Stuart L.;

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Abstract The ability of bacteria to sense specific molecules within their environment and trigger metabolic responses in accordance is an invaluable biotechnological resource. While many transcription factors (TFs) mediating such processes have been studied, only a handful have been leveraged for molecular biology applications. To expand the repertoire of biotechnologically relevant sensors we present a strategy for the construction and testing of chimeric TF libraries, based on the fusion of highly soluble periplasmic binding proteins (PBPs) with DNA-binding domains (DBDs). We validate this concept by constructing and functionally testing two unique sense-and-respond regulators for benzoate, an environmentally and industrially relevant metabolite. This work will enable the development of tailored biosensors for novel synthetic regulatory circuits.

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Research Organization:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-02ER63445

OSTI ID:: 1462285

Alternate ID(s):: OSTI ID: 1463113

Journal Information:: Nature Communications, Journal Name: Nature Communications Vol. 9 Journal Issue: 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 30 works

Citation information provided by
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