Low irradiance multiphoton imaging with alloyed lanthanide nanocrystals

Tian, Bining; Fernandez-Bravo, Angel; Najafiaghdam, Hossein; Torquato, Nicole A.; Altoe, M. Virginia P.; Teitelboim, Ayelet; Tajon, Cheryl A.; Tian, Yue; Borys, Nicholas J.; Barnard, Edward S.; Anwar, Mekhail; Chan, Emory M.; Schuck, P. James; Cohen, Bruce E.

doi:10.1038/s41467-018-05577-8

Title: Low irradiance multiphoton imaging with alloyed lanthanide nanocrystals

Journal Article · Mon Aug 06 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-05577-8· OSTI ID:1462284

Tian, Bining; Fernandez-Bravo, Angel; Najafiaghdam, Hossein; Torquato, Nicole A.; Altoe, M. Virginia P.; Teitelboim, Ayelet; Tajon, Cheryl A.; Tian, Yue; Borys, Nicholas J.;

; Anwar, Mekhail; Chan, Emory M.; Schuck, P. James;

Abstract Multiphoton imaging techniques that convert low-energy excitation to higher energy emission are widely used to improve signal over background, reduce scatter, and limit photodamage. Lanthanide-doped upconverting nanoparticles (UCNPs) are among the most efficient multiphoton probes, but even UCNPs with optimized lanthanide dopant levels require laser intensities that may be problematic. Here, we develop protein-sized, alloyed UCNPs (aUCNPs) that can be imaged individually at laser intensities >300-fold lower than needed for comparably sized doped UCNPs. Using single UCNP characterization and kinetic modeling, we find that addition of inert shells changes optimal lanthanide content from Yb ³⁺ , Er ³⁺ -doped NaYF ₄ nanocrystals to fully alloyed compositions. At high levels, emitter Er ³⁺ ions can adopt a second role to enhance aUCNP absorption cross-section by desaturating sensitizer Yb ³⁺ or by absorbing photons directly. Core/shell aUCNPs 12 nm in total diameter can be imaged through deep tissue in live mice using a laser intensity of 0.1 W cm ⁻² .

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Chinese Scholarship Council (CSC); Weizmann Inst. of Science (Israel)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1462284

Alternate ID(s):: OSTI ID: 1477411

Journal Information:: Nature Communications, Journal Name: Nature Communications Vol. 9 Journal Issue: 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 73 works

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