Title: Diverse lignocellulosic feedstocks can achieve high field‐scale ethanol yields while providing flexibility for the biorefinery and landscape‐level environmental benefits

Abstract

Increasing the diversity of lignocellulosic feedstocks accepted by a regional biorefinery has the potential to improve the environmental footprint of the facility; harvest, storage, and transportation logistics; and biorefinery economics. However, feedstocks can vary widely in terms of their biomass yields and quality characteristics (chemical composition, moisture content, etc.). To investigate how the diversity of potential biofuel cropping systems and feedstock supply might affect process and field-scale ethanol yields, we processed and experimentally quantified ethanol production from five different herbaceous feedstocks: two annuals (corn stover and energy sorghum) and three perennials (switchgrass, miscanthus, and mixed prairie). The feedstocks were pretreated using ammonia fiber expansion (AFEX), hydrolyzed at high solid loading (~17%–20% solids, depending on the feedstock), and fermented separately using microbes engineered to utilize xylose: yeast (Saccharomyces cerevisiaeY128) or bacteria (Zymomonas mobilis8b). The field-scale ethanol yield from each feedstock was dependent on biomass quality and cropping system productivity; however, biomass yield had a greater influence on the ethanol yield for low-productivity crops, while biomass quality was the main driver for ethanol yields from high-yielding crops. The process ethanol yield showed similar variability across years and feedstocks. A low process yield for corn stover was determined to result from inhibition ofmore » xylose utilization by unusually elevated levels of hydroxycinnamates (p-coumaric and ferulic acids) in the untreated biomass and their acid and amide derivatives in the resulting hydrolyzate. This finding highlights the need to better understand factors that influence process ethanol yield and biomass quality. Ultimately we provide evidence that most feedstocks fall within a similar range of process ethanol yield, particularly for the more resistant strain Z. mobilis8b. This supports the claim that the refinery can successfully diversify its feedstock supply, enabling many social and environmental benefits that can accrue due to landscape diversification.« less

Authors:

Zhang, Yaoping ^[1]; Oates, Lawrence G.  ^[2];

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Serate, Jose ^[1]; Xie, Dan  ^[1];

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Pohlmann, Edward ^[1]; Bukhman, Yury V. ^[1]; Karlen, Steven D. ^[1]; Young, Megan K. ^[1]; Higbee, Alan  ^[1];

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Eilert, Dustin ^[1]; Sanford, Gregg R. ^[2]; Piotrowski, Jeff S. ^[1]; Cavalier, David ^[3]; Ralph, John ^[4]; Coon, Joshua J. ^[5]; Sato, Trey K.  ^[1];

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Ong, Rebecca G.  ^[6]

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+ Show Author Affiliations

1. DOE‐Great Lakes Bioenergy Research Center University of Wisconsin‐Madison Madison Wisconsin
2. DOE‐Great Lakes Bioenergy Research Center University of Wisconsin‐Madison Madison Wisconsin, Department of Agronomy University of Wisconsin‐Madison Madison Wisconsin
3. DOE‐Great Lakes Bioenergy Research Center Michigan State University East Lansing Michigan
4. DOE‐Great Lakes Bioenergy Research Center University of Wisconsin‐Madison Madison Wisconsin, Department of Biochemistry University of Wisconsin‐Madison Madison Wisconsin
5. DOE‐Great Lakes Bioenergy Research Center University of Wisconsin‐Madison Madison Wisconsin, Department of Chemistry University of Wisconsin‐Madison Madison Wisconsin, Genome Center of Wisconsin University of Wisconsin‐Madison Madison Wisconsin
6. Department of Chemical Engineering Michigan Technological University Houghton Michigan, DOE‐Great Lakes Bioenergy Research Center Michigan Technological University Houghton Michigan

Publication Date:

Wed Jul 25 00:00:00 EDT 2018

Research Org.:

Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:

1461702

Alternate Identifier(s):

OSTI ID: 1461703; OSTI ID: 1483929; OSTI ID: 1506676

Grant/Contract Number:  

SC0018409

Resource Type:

Journal Article: Published Article

Journal Name:

Global Change Biology. Bioenergy

Additional Journal Information:

Journal Name: Global Change Biology. Bioenergy Journal Volume: 10 Journal Issue: 11; Journal ID: ISSN 1757-1693

Publisher:

Wiley-Blackwell

Country of Publication:

United Kingdom

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; Biorefinery; Ethanol yield; Feedstock Diversity; Fermentation; Inhibitors; lignocellulosic hydrolyzate; xylose utilization; biorefinery, ethanol yield, feedstock diversity, fermentation, inhibitors, lignocellulosic hydrolyzate, xylose utilization